- Beliveau BJ, Apostolopoulos N, Wu C. (2014) Visualizing Genomes with Oligopaint FISH Probes. Curr Protoc Mol Biol 105, 14.23.11-14.23.20.
Oligopaint probes are fluorescently labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D-FISH, and sample preparation.