- Zhao J, Zhao Y, Wang Z, Xuan Y, Luo Y, Jiao W, Cipolletta E, Citarella F, Barnaba V, Macino G. (2014) Loss expression of miRNA-200c induces adverse post-surgical prognosis of advanced stage Non-Small Cell Lung carcinoma and its potential relationship with ETAR mRNA. Thoracic Cancer 6(4), 421-426.
Non-small cell lung cancer (NSCLC) is the leading cause of cancer mortality worldwide. As miRNA-200 and ETBR may play an essential role in the process of EMT (epithelial to mesenchymal transition) simultaneously, the purpose of this study was to detect the expression of miRNA-200c and ETAR mRNA and assessed their prognostic significance in early stage NSCLC.
All the 78 advanced stage (IIB, IIIA, IIIB) NSCLC patients are smokers. By using quantitative reverse transcriptase PCR analysis, we detect the expression of miRNA-200c and ETBR mRNA and assess their correlation by χ2 test. TTP (time to progression) was used as recurrent index and was assessed by univariate and multivariate analysis in the Cox hazard model.
Both of miRNA-200c and ETAR mRNA expression are associated with N-stage and TNM-stage in a series of advanced NSCLC. Among N-stage and TNM-stage, significant differences are found in the groups of IIB (P=0.0126), IIIB (P=0.0107) and N0 (P=0.0023) and N1+N2 (P=0.0133). By both univariate and multivariate survival analyses, we found that miRNA-200c (HR=0.352, 95 %CI: 0.187-0.662) and ETAR mRNA (HR=2.500 95%CI: 1.345-4.647) were independent prognostic factors, independent of TNM-stage (HR = 2.414, 95 % CI: 1.600-3.642) and Differentiation (HR =1.530, 95%CI: 1.050-2230).
miRNA-200c induces an expedient surgical survival, whereas ETAR mRNA has the reverse prognosis in advanced stage Non–Small Cell Lung carcinoma and their potential relationship that miRNA-200c regulate EMT by Targeting ETAR mRNA.