Targeted NGS for investigating the mitochondrial genome and heteroplasmy
Disrupted mitochondrial functions and genetic variants of mitochondrial DNA (mtDNA) have been observed in different human neoplasms. Next-generation sequencing (NGS) can be used to detect even low heteroplasmy-level mtDNA variants.…
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Clinical Implication of MicroRNAs in Molecular Pathology
Sethi S, Ali S, Kong D, Philip PA, Sarkar FH. (2013) Clinical Implication of MicroRNAs in Molecular Pathology.Clinics in Laboratory Medicine 33(4):773-86.
The miRNAs have immense potential in the clinical arena because they can be detected in the blood; serum; tissues (fresh and formalin fixed paraffin embedded); and also fine-needle aspirate specimens.
Identification of novel molecular miRNAs and their target oncogenomic signatures have the potential to significantly impact clinical management.
Incorporating miRNA expression profiling on tissue samples in the future may not only confirm diagnosis categorizing diseases and their subtypes but also may predict drug response in helping clinicians define the precise therapy to each individual.
Increasing the knowledge of disease progression and tumor recurrence might also improve the development of personalized therapies.
The most attractive feature of miRNA-based therapy is that a single miRNA could be useful for targeting multiple genes that are deregulated in cancers, which can be further investigated through systems biology and network analysis that will allow designing cancer-specific personalized therapy.