- Weng T. (2019) Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation. Journal of Clinical Investigation 129(5), 1984-1999.
Idiopathic pulmonary fibrosis (IPF) is a deadly disease with a poor prognosis and few treatment options. Pathological
remodeling of the extracellular matrix (ECM) is a key factor that drives the disease pathogenesis, although the underlying
mechanisms remain unknown. Alternative polyadenylation (APA) has recently been shown to play a major role in cellular
responses to stress by driving the expression of fibrotic factors through the alteration of miRNA sensitivity, but a connection
to IPF has not been established. Here, we demonstrated that CFIm25, a global regulator of APA, was downregulated in the
lungs of patients with IPF and mice with pulmonary fibrosis, with its expression selectively reduced in α–smooth muscle
actin–positive (α-SMA–positive) fibroblasts. Following CFIm25 knockdown in healthy human lung fibroblasts, we identified
808 genes with shortened 3′-UTRs, including those involved in the TGF-β signaling pathway, the Wnt signaling pathway,
and cancer pathways. The expression of key profibrotic factors was suppressed by CFIm25 overexpression in IPF fibroblasts.
Finally, we demonstrated that deletion of CFIm25 in fibroblasts or myofibroblast precursors using either the Col1a1 or
the Foxd1 promoter enhanced pulmonary fibrosis after bleomycin exposure. Collectively, our results identified CFIm25
downregulation as an important mechanism for elevating profibrotic gene expression in pulmonary fibrosis.