In a recent study, small RNA libraries were constructed from an M. salmoides population and sequenced using deep sequencing technology.

A total of 9,888,822; 8,519,365; 20,566,198; and 15,762,254 raw reads representing 666,097; 755,711; 978,923; and 840,175 unique sequences were obtained from the spleen, liver, kidney, and muscle libraries, respectively. As a result, 509 known miRNAs belonging to 143 families and 1157 novel miRNAs were identified. The miRNAs displayed diverse expression levels among the four libraries, among which most of the known miRNAs were expressed at higher levels than the novel miRNAs. Furthermore, stem-loop qRT-PCR was applied to validate and profile the expression of the differentially expressed miRNAs in the four different tissues, which revealed that some miRNAs showed tissue specific expression.

The identification of miRNAs in M. salmoides provides new information and enhances our understanding of the functions of miRNAs in regulating biological processes.

LC Sciences

 

Reference
W Gong, Y Huang, J Xie, G Wang, D Yu, K Zhang, Z Li et al. (2018) Identification and comparative analysis of the miRNA expression profiles from four tissues of Micropterus salmoides using deep sequencing Genomics doi: 10.1016/j.ygeno.2018.09.017 [abstract]

New Sequencing Data Provides Resource for Studying How Plants Adapted to Drought Identification of Novel Biomarkers for Hepatocellular Carcinoma Using Transcriptome Analysis