Small RNA libraries were prepared from vesicle-enriched and vesicle-depleted supernatants from both adult worms and L4 stage larvae. Comparison of the miRNA species in the different fractions indicated that specific miRNAs are packaged within vesicles, while others are more abundant in vesicle-depleted supernatant. Hierarchical clustering analysis indicated that the gut is the likely source of vesicle-associated miRNAs in the L4 stage, but not in the adult worm.
These findings add to the growing body of work demonstrating that miRNAs released from parasitic helminths may play an important role in host-parasite interactions.
Developmental expression of the Hco-miR-5352 cluster across the H. contortus life cycle as assessed by microarray
Data for sheathed L3, activated L3 (act) (exsheathed and cultured at 37°C for 24 hours), L4 stage collected 7 days p.i., adult males and females, collected at 28 days p.i. and gut tissue extracted from adult female worms.