In their study, a total of 31 and 48 miRNAs were identified to be differentially expressed in the leaves and tubers, respectively. Among the DEGs, 1353 genes in the leaves and 1841 genes in the tubers were upregulated, while 1595 genes in the leaves and 897 genes in the tubers were downregulated by light. Mapman enrichment analyses showed that genes related to MVA pathway, alkaloids-like, phenylpropanoids, flavonoids, and carotenoids metabolism were significantly upregulated, while genes associated with major CHO metabolism were repressed in the leaves and tubers. Integrated miRNA and mRNA profiles revealed that light-responsive miRNAs were important regulators in alkaloids metabolism, UMP salvage, lipid biosynthesis, and cellulose catabolism. Moreover, several miRNAs may participate in glycoalkaloids metabolism via JA signaling pathway, UDP-glucose biosynthesis and hydroxylation reaction.
Numbers of CT-specific reads, RT-specific reads, and common reads between CT and RT libraries. Venn diagrams represent genome-matched total clean reads (a) and unique reads (b) between control library (CT) and light-treated (RT) in the tuber
This study provides a global view of miRNA and mRNA expression profiles in potato response to light. These results suggest that miRNAs might play important roles in secondary metabolic pathways, especially in glycoalkaloid biosynthesis. The findings improve our understanding of the genetic regulation of secondary metabolite pathways and pave the way for future application of genetically engineered potato.