DNA methylation is a key epigenetic modification that can regulate gene expression. Genomic DNA hypomethylation is commonly found in many gastrointestinal (GI) diseases. Dysregulated gene expression in GI smooth muscle cells (GISMCs) can lead to motility disorders. However, the consequences of genomic DNA hypomethylation within GI-SMCs are still elusive. Utilizing a Cre-lox murine model, a team led by researchers from the University of Nevada have generated SMC-restricted DNA methyltransferase 1 (Dnmt1) knockout (KO) mice and analyzed the effects of Dnmt1 deficiency...
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Enteric bacteria contribute to nitrogen balance in diverse vertebrates because they produce urease, the enzyme needed to liberate nitrogen from urea. Although this system of urea-nitrogen recycling is as yet unknown in Amphibia, a recent study by researchers from Miami University of the wood frog (Rana sylvatica), a terrestrial hibernator that is strongly hyperuremic during winter, documented robust urease activity in bacteria inhabiting the hindgut...
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MicroRNAs (miRNAs) are involved in growth, development, and occurrence and progression of many diseases. MiRNA-mediated post-transcriptional regulation is poorly understood in vascular biology and pathology. The purpose of a recent study by researchers from Texas Tech University Health Sciences Center was to determine circulatory miRNAs as early detectable peripheral biomarkers in patients with ischemic stroke (IS)...
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MicroRNAs (miRNA) play a pivotal role in regulating a broad range of biological processes, acting by cleaving mRNAs or by translational repression. However, the miRNAs from skin of Andrias davidianus have not been reported. In a recent study, a small-RNA cDNA library was constructed and sequenced from skin of A. davidianus...
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Gain-of-function mutations and overexpression of KIT are characteristic features of gastrointestinal stromal tumors (GIST). Dysregulation in miRNA expression may lead to KIT overexpression and tumorigenesis. Researchers recently utilized miRNA microarray technology and real-time PCR to determine the miRNA expression profiles in a cohort of 69 clinical samples...
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A 10 minute video that walks you step-by-step through the files you receive from our miRNA sequencing service – LC Sciences’ miRNA sequencing service includes all biostatistical tests for expression analysis, target GO and KEGG enrichment analysis and miRNA network analysis. The results of our data analysis are presented in a user friendly HTML file which helps our customers to save significant down-stream cost by quickly zooming in on relevant target miRNA transcripts for further studies...
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Magnolias are widely cultivated for their beautiful flowers, but despite their popularity, the molecular mechanisms regulating flower bud differentiation have not been elucidated. In a recent study, researchers used paraffin sections and RNA-seq to study the process of flower bud differentiation in Magnolia sinostellata...
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To counteract host immunity, Cryptosporidium parvum has evolved multiple strategies to suppress host antimicrobial defense. One such strategy is to reduce the production of the antimicrobial peptide beta-defensin 1 (DEFB1) by host epithelial cells but the underlying mechanisms remain unclear. Recent studies demonstrate that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected host cells and may modulate host gene transcription...
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Cerebral artery hypoperfusion may provide the basis for linking ischemic stroke with hypertension. Brain hypoperfusion may induce hypertension that may serve as an auto-protective mechanism to prevent ischemic stroke. A team led by researchers from University of Bristol hypothesised that hypertension is caused by remodelling of the cerebral arteries, which is triggered by inflammation...
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To explore the correlation of microRNA (miRNA) profiles with disease risk and severity in patients with allergic rhinitis (AR), researchers profiled miRNA expression in nasal mucosa samples from 8 AR patients and 8 matched non-atopic controls by miRNA microarray...
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