Clinical Implication of MicroRNAs in Molecular Pathology
MicroRNAs (miRNAs) are poised to provide diagnostic, prognostic, and therapeutic targets for several diseases including malignancies for precision medicine applications. The miRNAs have immense potential in the clinical arena because…
LC Sciences is a global biotechnology company providing products and services to genomics and proteomics researchers across an array of markets for nucleic acid/protein analysis, biomarker-discovery and drug development.
One-Step workflow makes targeting your genes of interest simple and efficient.
Genomic DNA Sample
In a single tube, a pair of common primers are added into a mixture of multiple pairs of specific primers and genomic DNA to form a single step, two stage multiplex PCR reaction.
A single PCR run accomplishes two functionally separated reaction phases, namely target replication and library amplification. No primer removal or any other enzymatic steps are required. This results in a significantly simplified work flow.
There is no need for a subsequent library preparation step (or added cost) as thr Relay-PCR reaction will yield sequencing ready libraries.
Sample bar-codes, sequencing adapters, and molecular bar-codes are all incorporated during the one-step Relay-PCR reaction.