LC Sciences is a global biotechnology company providing products and services to genomics and proteomics researchers across an array of markets for nucleic acid/protein analysis, biomarker-discovery and drug development.
2575 West Bellfort Street Suite 270 Houston, TX 77054 USA
LC Bio 4F, Building 16, No.260 6th Avenue, HEDA, Hangzhou 310018
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VariantPro™ Targeted Sequencing – Workflow
One-Step workflow makes targeting your genes of interest simple and efficient.
Genomic DNA Sample
In a single tube, a pair of common primers are added into a mixture of multiple pairs of specific primers and genomic DNA to form a single step, two stage multiplex PCR reaction.
A single PCR run accomplishes two functionally separated reaction phases, namely target replication and library amplification. No primer removal or any other enzymatic steps are required. This results in a significantly simplified work flow.
There is no need for a subsequent library preparation step (or added cost) as thr Relay-PCR reaction will yield sequencing ready libraries.
Sample bar-codes, sequencing adapters, and molecular bar-codes are all incorporated during the one-step Relay-PCR reaction.