Application Note

Chip Design

  • Epitope (known) DYKDDDDK
  • 312 Epitope and variant sequences
  • >4 redundancy
  • CV < 15%

Assay

  • Antibody: anti-flag peptide (AFM2) (1 ng/ml)
  • 200 µl TBS
  • Temp: 4oC
  • Reaction time: 1 hour

Detection

  • Cy3

Results

  • Figure 1 – Fluorescent image of 4K peptide chip from AFM2 binding

Figure 1

  • Figure 2 – Plot of the signal intensities of the epitope sequence variants

Figure 2

DYKDDDDKRed and Pink: most or somehow important residues in the 8-mer epitope.DYKDDDDKBlue: residues dispensable from both ends.


Quantitative Measurement of Anti c-myc Epitope Peptide Binding Peptide Microarray Data Analysis Using SVM-PEPARRAY