LC Sciences provides a fast, one-stop whole transcriptome sequencing solution – from your sample through data analysis.
Whole Transcriptome Sequencing (WTS), which includes RNA-Seq with rRNA removal and small RNA-Seq, is used to reveal the complete picture of mRNAs, lncRNAs, circRNAs, as well as microRNA (miRNA), in a biological sample under specific conditions. WTS enables exploration of a complicated post-transcriptional regulation mechanism referred to as a competitive endogenous RNA (ceRNA) network. These ceRNAs such as mRNAs, lncRNAs, circRNAs act as molecular sponges for a miRNA through their miRNA binding sites, thereby de-repressing all target genes of the respective miRNA family.
Our Comprehensive Services Offer:
- The latest in next-gen sequencing technology
- Experienced staff to assist you with experimental design
- Fast turnaround times
- Full bioinformatics analysis
- Expert technical support to assist with data interpretation
Advanced Data Analysis
Profile RNA in any species – there is no need for probes based on prior sequence or secondary structure information.
Profiling is transcriptome-wide – investigate all RNAs, known and unknown, in your sample.
Achieve digital, quantitative transcript expression analysis – Accurate expression levels over 5 orders of magnitude.
Detect novel and rare transcripts and transcript variants, such as single nucleotide mutations (SNP)
Total RNA amount ≥ 2 μg
260/280 >1.8, 260/230 >1.0
RIN value ≥ 7.0
Size Fractionation Selection
18~40 bp Insert cDNA Library Ribosomal RNA Removal
250~300 bp Insert cDNA Library
50 bp Single End
7-10 Million Reads
150 bp Paired End
>80 Million Reads
High Data Quality
Data quality is measured by the percentage of bases with a sequencing quality score above Q30.
These comprehensive services are designed to be one-stop and produce the results needed to quickly advance your biological and biomedical research. For all types of RNA sequencing, our “Total RNA to Data” services include: sample prep/QC, library preparation, RNA sequencing, bioinformatics analysis, and high-level customer support.
LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into RNA research. We don’t merely deliver data; we will handle all the advanced bioinformatics analysis and help you find answers to the questions you’re asking.