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        • Featured Customer Publication

          Construction of a scFv Library with Synthetic, Non-combinatorial CDR Diversity
          Construction of a scFv Library with Synthetic, Non-combinatorial CDR Diversity
          Many large synthetic antibody libraries have been designed, constructed, and successfully generated high-quality antibodies suitable for various demanding applications...
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        • The Latest Customer Publications

          Long noncoding RNA GAS5 inhibits progression of colorectal cancer by interacting with and triggering YAP phosphorylation and degradation and is negatively regulated by the m6A reader YTHDF3
          YTHDF1 links hypoxia adaptation and non-small cell lung cancer progression
          MicroRNA528, a hub regulator modulating ROS homeostasis via targeting of a diverse set of genes encoding copper-containing proteins in monocots
          The effect of cadmium on the microRNAome, degradome and transcriptome of rice seedlings
          Revealing of MicroRNA Involved Regulatory Gene Networks on Terpenoid Biosynthesis in Camellia sinensis in Different Growing Time Points
          Investigation on the role of gene hp0788 in Helicobacter pylori in infecting gastric epithelial cells
          Integrated transcriptome, small RNA, and degradome analysis reveals the complex network regulating starch biosynthesis in maize
          Transcriptome sequencing reveals phagocytosis as the main immune response in the pathogen-challenged sea urchin Strongylocentrotus intermedius
          Aberrant ERG expression associates with downregulation of miR-4638-5p and selected genomic alterations in a subset of diffuse large B-cell lymphoma
          Analysis of the microRNA expression profiles in feline kidney cell line infected with feline panleukopenia virus
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           Identification of Novel Protein Interaction Networks in Tyrosine Kinase Signaling Pathways
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          Targeted NGS for investigating the mitochondrial genome and heteroplasmy

           Targeted NGS for investigating the mitochondrial genome and heteroplasmy

          Disrupted mitochondrial functions and genetic variants of mitochondrial DNA (mtDNA) have been observed in different human neoplasms. Next-generation sequencing (NGS) can be used to detect even low heteroplasmy-level mtDNA variants.…

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        • The Latest News

          RNA sequencing reveals gene network regulated terpenoid biosynthesis in tea plant (Camellia sinensis)
          Join LC Sciences at ASHG 2019 – Oct 15-19 – Houston, TX
          Researchers use CRISPR screening to define a diverse landscape of DNA damage response gene mutations
          Extracellular vesicle contents in synovial fluid may serve as a diagnostic for arthritic disease and indicator for therapeutic efficacy of senolytic treatment
          Researchers demonstrate the feasibility of a microRNA-based therapy for promoting wound closure
          Researchers unravel a causal link between cocaine use, gut-barrier dysfunction and dysbiosis
          RNA Sequencing reveals Wipi1 a potential target for right ventricular-directed heart failure therapy
          Targeted NGS is a reliable method for investigating the mitochondrial genome and heteroplasmy
          Extracellular vesicle contents found in synovial fluid may serve as a diagnostic for arthritic disease
          Extracellular MiRNA Cargo Play Important Role in Manipulation of Host Phenotype During Parasitic Infection
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          LC Sciences is a global biotechnology company providing products and services to genomics and proteomics researchers across an array of markets for nucleic acid/protein analysis, biomarker-discovery and drug development.
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          VariantPro™ Targeted Sequencing Technology

          VariantPro™ Targeted Sequencing Technology

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Support Documents

  1. Home
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  3. Transcriptomics
  4. microRNA Discovery & Profiling
  5. microRNA qRT-PCR Service
  6. Support Documents

Application Notes

MicroRNA Sample Preparation from Biofluid Samples

MicroRNA Sample Preparation from Biofluid Samples

The study of basic microRNA (miRNA) function has been performed mainly through collection of tissues by invasive methods. However, in a clinical setting…

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MicroRNA Sample Preparation from Serum/Plasma Samples

MicroRNA Sample Preparation from Serum/Plasma Samples

The study of basic microRNA (miRNA) function has been performed mainly through collection of tissues by invasive methods. However, in a clinical setting…

read more

MicroRNA expression analysis of blood samples can further our understanding of diseases of the blood

MicroRNA expression analysis of blood samples can further our understanding of diseases of the blood

The regulatory effects microRNA of are widespread and microRNAs have recently been implicated in various diseases of the blood, stem cells, brain, and…

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microRNA profiling of FFPE tissue samples

microRNA profiling of FFPE tissue samples

Archiving histological tissue samples by formalin-fixing and embedding in paraffin blocks has been a routine practice for medical and research…

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MicroRNA Profiling Increases Understanding of Molecular Circuitry

MicroRNA Profiling Increases Understanding of Molecular Circuitry

The mechanism of regulation by microRNA (miRNA) is complex in that miRNAs are frequently transcribed together as primary transcripts that are then…

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Integrating microRNA and mRNA expression profiles for identification of regulatory networks

Integrating microRNA and mRNA expression profiles for identification of regulatory networks

The discovery of microRNA as an additional regulatory mechanism has been a revolution to the field of Developmental Biology. While early research has…

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MicroRNAs as Potential Biomarkers in Cancer and Heart Disease

MicroRNAs as Potential Biomarkers in Cancer and Heart Disease

Heart disease and cancer represent the number one and number two killer diseases in developed countries. Identifying biomarkers to detect these and other…

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The many complex roles of microRNA in breast cancer

The many complex roles of microRNA in breast cancer

There have been several studies involving microRNAs and breast cancer to date. These studies collectively demonstrate the wide range of functions…

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microRNAs in Molecular Medicine

microRNAs in Molecular Medicine

Molecular Diagnostics / Biomarkers - Identification of specific miRNAs or miRNA expression based signatures that can act as biomarkers…

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MicroRNA Discovery and Validation

MicroRNA Discovery and Validation

The study of microRNA (miRNA) is growing rapidly as researchers discover new miRNAs and uncover the importance of these small regulatory elements…

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Technical Bulletins

Biological Replicate Considerations - Differential Expression Analysis

Biological Replicate Considerations - Differential Expression Analysis

Although next-generation sequencing and microarray service is becoming more and more affordable every year, these projects still represent a significant...

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Total RNA Sample Preparation for RNA Analysis Studies

Total RNA Sample Preparation for RNA Analysis Studies

The amount of total RNA required for library preparation depends on the profiling service requested. Please see our RNA sequencing service comparison chart…

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International Shipping Instructions - Total RNA Samples

International Shipping Instructions - Total RNA Samples

LC Sciences provides the following information to facilitate the international shipment of total RNA samples. We serve many customers overseas and …

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Validation of miRNA Microarray Results with Real-Time QPCR

Validation of miRNA Microarray Results with Real-Time QPCR

The time to start thinking about validating your microarray results is not after you have completed your experiment, but…

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Customer Quality Control Checks of RNA Sample

Customer Quality Control Checks of RNA Sample

Quality of total RNA samples is upmost important to the success of miRNA array assays. Three quality controls are performed including quantity, purity…

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Total RNA Preparation for microRNA Analysis Studies

Total RNA Preparation for microRNA Analysis Studies

For each sample, we ask that you try to send in at least 1-3 µg of total RNA. Please transfer your sample…

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Product Literature

Product Brochure – microRNA Discovery & Profiling Services

Product Brochure – microRNA Discovery & Profiling Services

LC Sciences provides a comprehensive sequencing service utilizing Illumina,s high-throughput sequencing technology which enables comprehensive, highly sensitive…

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Technical Information

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US Headquarters

West Bellfort Street
Suite 270
Houston, TX
77054 USA

Local (713) 664-7087
Toll Free: 1-888-528-8818
Fax: (713) 664-8181
www.lcsciences.com

About Us

LC Sciences is a global biotechnology company providing products and services to genomics and proteomics researchers across an array of markets for nucleic acid/protein analysis, biomarker-discovery and drug development.

The Latest News

RNA sequencing reveals gene network regulated terpenoid biosynthesis in tea plant (Camellia sinensis)
Join LC Sciences at ASHG 2019 – Oct 15-19 – Houston, TX
Researchers use CRISPR screening to define a diverse landscape of DNA damage response gene mutations
Extracellular vesicle contents in synovial fluid may serve as a diagnostic for arthritic disease and indicator for therapeutic efficacy of senolytic treatment
Researchers demonstrate the feasibility of a microRNA-based therapy for promoting wound closure
Researchers unravel a causal link between cocaine use, gut-barrier dysfunction and dysbiosis

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