lncRNA Sequencing Service
LC Sciences provides a fast, one-stop lncRNA sequencing solution – from your sample through data analysis.
lncRNAs are transcribed RNAs longer than 200 nt and do not encode for proteins. However, lncRNA are involved in a wide range of important cellular processes and play a critical role in human diseases such as cancer, cardiovascular disease and neurological disorders. lncRNA-Seq reveals the complete picture of the lncRNA in a biological sample under specific conditions.
Our Comprehensive Services Offer:
- The latest in next-gen sequencing technology
- Experienced staff to assist you with experimental design
- Fast turnaround times
- Full bioinformatics analysis
- Expert technical support to assist with data interpretation
Advanced Data Analysis
Profile lncRNA in any species – there is no need for probes based on prior sequence or secondary structure information.
Profiling is transcriptome-wide – investigate all lncRNAs, known and unknown, in your sample.
Achieve digital, quantitative transcript expression analysis – Accurate expression levels over 5 orders of magnitude.
Detect novel and rare transcripts and transcript variants, such as single nucleotide mutations (SNP)
In-Depth lncRNA Data Analysis Includes:
- Complete mRNA analysis – see Poly(A) RNA-seq
- lncRNA profiling – expression analysis, length distribution, etc
- Candidate novel lncRNAs
- Statistics of different types of class codes (j, i, o, u, x)
- Statistics of different CPC (coding potential caculator) and CNCI (coding non-coding index) scores
- Localization of lncRNAs on genome
- Basic property comparison of lncRNA and mRNA
- Differential expression of lncRNAs
- Target gene prediction and functional analysis of lncRNAs
Total RNA amount ≥ 2 μg
260/280 >1.8, 260/230 >1.0
RIN value ≥ 7.0
Ribosomal RNA Removal
250~300 bp Insert cDNA Library
150 bp Paired End
>80 Million Reads
High Data Quality
Data quality is measured by the percentage of bases with a sequencing quality score above Q30.
These comprehensive services are designed to be one-stop and produce the results needed to quickly advance your biological and biomedical research. For all types of RNA sequencing, our “Total RNA to Data” services include: sample prep/QC, library preparation, RNA sequencing, bioinformatics analysis, and high-level customer support.
LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into RNA research. We don’t merely deliver data; we will handle all the advanced bioinformatics analysis and help you find answers to the questions you’re asking.