The mitochondrial genome is a circular DNA molecule which is distinct from the nuclear genome. In humans, it’s about 16 kb long and encodes 37 genes. Mitochondrial disorders arise as a result of dysfunction of the mitochondrial respiratory chain, and often times, these are caused by mutations of genes encoded by mitochondrial DNA (mtDNA). Because there is considerable clinical variability between mitochondrial disorders and many patients who exhibit phenotypes that overlap diseases, often diagnosis may only be confirmed by identification of a pathogenic mtDNA variant through molecular genetic testing of DNA extracted from a blood sample.
|Description||The assay kit contains all the reagents required for capture of the entire human mitochondrial DNA genome and library preparation for next-gen sequencing|
|Capture Method||The VariantPro™ system incorporates novel Relay-PCR™ and Omega Primer™ technologies to form a one-step multiplex PCR based workflow.|
|Amplicon Coverage||Designed for 100% amplicon coverage of all regions of the mitochondrial genome (16,569 bp)|
|Amplicon Length||Average 200 bp|
|Primer Pools||110 pairs of primers in 2 primer pair pools|
|Input DNA Required||1-5 ng human genomic DNA per reaction tube (2) – depending on the DNA quality|
|Assay Time||4-6 hours|
|Hands-on Time||5 minutes|
|Multiplexing Capability||Up to 3024 samples in one lane|
|Coverage Uniformity||> 99% at 0.2 x mean|
VariantPro targeted sequencing system is based on a novel multiplex PCR technology that combines major innovations to facilitate simple operation.…
Refer to User Guide – VariantPro Amplicon Design Files for detail of the amplicon design scheme. geneName: MT-CO1;MT-TS1;MT-TD;MT-CO2…
In a single tube, a pair of common primers are added into a mixture of multiple pairs of specific primers and genomic DNA to form a single step, two stage multiplex PCR reaction….
More performance data can be found here – Technical Note – Performance of the VariantPro Mitochondrial Panel