Because many natural microorganisms cannot be isolated and cultured or cloned, the vast majority of microbial biodiversity has been missed by traditional cultivation-based analysis methods. New next-gen DNA sequencing technologies enable us to directly sequence all genetic material that is recovered from all the members of a sampled community, including unculturable microorganisms that are otherwise difficult to analyze. This method provides, not only the species identification, classification and quantitation of microbes but also provides information for functional analysis of all the genes through gene ontology and metabolic pathway analysis.

Sample Data

  1. Captures the entire biodiversity of complex samples.
  2. Allows the simultaneous study of bacteria, archea, viruses, and single celled eukaryotes.
  3. Enables a more specific taxonomic and functional classification.
  4. Reveals the functional ecology of environmental communities on a massive scale.
  5. Provide insight into the function of complex microbial communities and their role in host health.
  6. Enables the study of metabolic pathways to explore the relationship between microorganisms and the environment.

Metagenomics Sequencing

Sample Requirements

Total DNA amount ≥ 5 μg
Conc ≥ 30 ng/μl

Library
Preparation

RNA Selection – Size Fractionation
300 bp Insert DNA Library

Recommended
Sequencing Depth

150 bp Paired End
~40 Million Reads

High Data Quality

Data quality is measured by the percentage of bases with a sequencing quality score above Q30.