To counteract these alterations, investigators replenished let-7i miRNA by intranasal administration; in a separate experiment, they added an anti-miR that antagonized elevated mmu-341 after 5 months of diabetes. Both approaches were shown to independently improve electrophysiologic, structural, and behavioral abnormalities without altering hyperglycemia; control sequences did not have those effects. Researchers found that dissociated adult sensory neurons exposed to an exogenous mmu-let-7i mimic displayed enhanced growth and branching, indicating a trophic action. These findings identify roles for epigenetic miRNA alterations and enhanced GW/P expression in diabetic dorsal root ganglia that contribute to the complex DPN phenotype.
Heat map results comparing DRG miRNA microarrays of 5-month-old mice with diabetes and mice without diabetes at the p < 0.05 (a) and p < 0.01 significance levels (b). (c) Selected higher abundance miRNAs are shown indicating fold change and significance level at p < 0.10. * p < 0.05 and ** p < 0.01, Student t-test; n = 6 control mice; n = 6 mice with diabetes (for each mouse DRG, n = 1). (d) Independent miRNA qRT-PCR analysis of relative mmu-let-7i levels confirms its reduction in diabetic DRG. * p = 0.001, Student t-test; n = 6 nondiabetic arrays; n = 5 diabetic arrays.
miRNA Microarray Service – LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house developed µParaflo® technology platform. We have standard arrays for all mature miRNAs of all species available in the latest version of the miRBase database (Release 21, July 2014). Our service is comprehensive and includes sample labeling, array hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we’ll send you both the raw and fully analyzed data. [Learn more…]
Cheng C, Kobayashi M, Martinez JA, Ng H, Moser JJ, Wang X, Singh V. Fritzler MJ, Zochodne DW. (2015) Evidence for Epigenetic Regulation of Gene Expression and Function in Chronic Experimental Diabetic Neuropathy J Neuropathol Exp Neurol 74(8):804-17 [article]