1. Start with as little as 10 pg–10 ng of total RNA
  2. Library preparation – poly(A) tail selection method
  3. Illumina Hi-Seq 2000 – 2 x 100bp paired-end sequencing run
  4. Advanced bioinformatics package

Next-Gen DNA Sequencer

LC Sciences provides a transcriptome profiling service using the Illumina high-throughput sequencing technology which enables comprehensive coverage, highly sensitive and specific discovery and profiling of all forms of RNAs in your sample.

This service is comprehensive – from sample to data, providing advanced technology and years of experience in efficient discovery and profiling of RNAs.

Our comprehensive profiling service includes:

  • Sample QC
  • Sample preparation (reverse transcriptase template switching)
  • Library preparation (single molecule clonal amplification)
  • High-throughput sequencing (RNA-Seq method, Illumina Next-Gen sequencing technology)
  • Bioinformatics analysis
  • Customer data report

Illumina base-calling and analysis
Raw sequencing data – 50bp single-end reads – either FASTA-A or FASTA-Q files with base-calling quality scores
LC Sciences quality filtered data reduced to mappable reads, a list of unique sequences and their copy numbers
Genomic mapping of quality sequencing reads
Test for differential expression at gene level and transcript level. (biological replicates required – see Technical Note – Biological Replicate Considerations – Differential Expression Analysis)
GO and KEGG annotation and enrichment analysis.
Customer data report – includes a summary of methods and all statistic analysis

User friendly HTML customer data report – includes a summary of methods and all statistic analysis

Sample Data