Poly(A) RNA Sequencing Service
LC Sciences provides a fast, one-stop Poly(A) RNA sequencing solution – from your sample through data analysis.
Our Comprehensive Services Offer:
- The latest in next-gen sequencing technology
- Experienced staff to assist you with experimental design
- Fast turnaround times
- Full bioinformatics analysis
- Expert technical support to assist with data interpretation
Library Preparation
Next-Gen Sequencing
Advanced Data Analysis
Any Species
Profile mRNA in any species – there is no need for probes based on prior sequence or secondary structure information.
Transcriptome-wide
Profiling is transcriptome-wide – investigate all mRNAs, known and unknown, in your sample.
Digital Results
Achieve digital, quantitative transcript expression analysis – Accurate expression levels over 5 orders of magnitude.
Novel Transcripts
Detect novel and rare transcripts and transcript variants, such as single nucleotide mutations (SNP)
In-Depth Data Analysis Includes:
- Alignment, classification, & functional annotation of all mapped reads
- Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
- Prediction of novel transcripts
- GO and KEGG annotation and enrichment analysis
- Statistics and annotation of SNPs/Indels
- Alternative splicing analysis
Sample Data Report
Analysis Pipeline
Sample Requirements
Total RNA amount ≥ 2 μg
260/280 >1.8, 260/230 >1.0
RIN value ≥ 7.0
Recommended
Sequencing Depth
150 bp Paired End
>40 Million Reads
High Data Quality
Data quality is measured by the percentage of bases with a sequencing quality score above Q30.
Turnkey Solution
These comprehensive services are designed to be one-stop and produce the results needed to quickly advance your biological and biomedical research. For all types of RNA sequencing, our “Total RNA to Data” services include: sample prep/QC, library preparation, RNA sequencing, bioinformatics analysis, and high-level customer support.
Expert Services
LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into RNA research. We don’t merely deliver data; we will handle all the advanced bioinformatics analysis and help you find answers to the questions you’re asking.