New RNA sequencing methods enable discovery and profiling of RNAs in any organism without prior genome annotation. Rapid advancement of new technologies has led to the development of several methods of RNA sequencing which differ in many respects such as: the RNA selection method, required sequencing depth, and type of data generated. The purpose of this video is to explain the differences between the various RNA sequencing services that LC Sciences offers, and help you determine which service is right for your project based on the goals of your experiment(s).

Description Poly(A) RNA-Seq Single-Cell
(Low Input) RNA-Seq
Total RNA-Seq microRNA-Seq Degradome Sequencing lncRNA-Seq circRNA-Seq circRNA-Seq with ID Whole Transcriptome Sequencing
RNA Species mRNA mRNA mRNA microRNA mRNA fragments mRNA + lncRNA mRNA + lncRNA + circRNA circRNA mRNA, miRNA, lncRNA, circRNA
RNA Selection Method PolyA-Tail Selection PolyA-Tail Selection rRNA Depletion Size Fractionation PolyA-Tail Selection – mRNA fragments rRNA Depletion rRNA Depletion rRNA Depletion & RNase R Digest rRNA Depletion & Size Fractionation
Data Analysis Discovery, Expression, Annotation Expression, Annotation Discovery, Expression, Annotation Discovery, Expression, Annotation miRNA Target ID, Degradation Plots Discovery, Expression, Annotation Discovery, Expression, Annotation Discovery, Expression, Annotation Discovery, Expression, Annotation
Sequencing Instrument HiSeq 4000 HiSeq 4000 HiSeq 4000 HiSeq 4000 HiSeq 4000 HiSeq 4000 HiSeq 4000 HiSeq 4000 HiSeq 4000
Sequencing Run Length 150bp Paired-end 150bp Paired-end 150bp Paired-end 50bp Single-end 50bp Single-end 150bp Paired-end 150bp Paired-end 150bp Paired-end 150bp Paired-end & 50bp Single-end
Recommended Sequencing Depth 40 million 40 million 40 million 7-10 million 7-10 million 80 million 80 million 40 million 80 million & 7-10 million
Type of Sample Total RNA Total RNA Total RNA Total RNA Total RNA Total RNA Total RNA Total RNA Total RNA
Pref Sample Amount 4 µg 10 ng 4 µg 1 µg 10 µg 4 µg 4 µg 4 µg 4 µg
Min Sample Amount Required 2 µg 10 pg 2 µg 100 ng 5 µg 2 µg 2 µg 2 µg 2 µg

* Inquire about immuno-precipitated RNAs or other enriched RNAs.