RNA Sequencing Quote Request Form | LC Sciences

[PART 1] - Project Objectives

Name

First Last

Institution*

Email*

Phone

Purpose of the Sequencing Project

microRNA/Small RNA Sequencing
Degradome Sequencing
mRNA expression profiling of known genes
Single-cell mRNA expression profiling
mRNA expression profiling, novel poly-A transcript discovery and alternative splicing info
mRNA expression profiling, novel transcript discovery, alternative splicing and non-coding RNA info
Other (explain)

Describe the main objective of the project

[PART 2] - Sample Description

Number of Samples*

Type of Material

Cultured cells
Total RNA
poly-A mRNA
Small RNA
Other (please describe)

Amount of RNA (Approx.)

Number of Cells (Approx.)

Sample Species (Latin name):*

Sample Isolation Method:

Additional Sample Description:

[PART-3] Data Analysis Required

microRNA/Small RNA Sequencing

Standard Data Filtering (Base-calling analysis & LC Sciences filtering to mappable reads - no further data processing or analysis)
Advanced microRNA Analysis
• Custom construction of reference database(s) – miRBase, genome, etc and mapping of all quality reads
• Alignment, classification, & functional annotation of all mapped reads
• Prediction of possible novel miRs
• Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
• Conservation profile of the identified miRNAs analysis
• Pre-miRNA clusters
• miRNA target prediction
• miRNA target GO and KEGG enrichment analysis
Other (please explain)

Degradome Sequencing

Raw Data (standard base-calling & filtering - no further data processing or analysis)
Advanced Degradome Analysis
• A list of unique sequences and their copy numbers
• Identification of rRNAs, tRNAs, snRNAs, snoRNAs, polyN and other non-coding RNAs
• Distribution of degradation fragments on selected region of genome
• Identification of target mRNAs
• Statistical summary of mRNA degradation sites
• Identification of degradation mRNA related microRNAs from miRBase (Results will be shown in a target plot diagram.)
Other (please explain)

Single-cell mRNA Expression

Raw Data (standard base-calling & filtering - no further data processing or analysis)
Standard mRNA Expression Analysis Package
• A list of unique sequences and their copy numbers
• Genomic mapping of quality sequencing reads
• Test for differential expression at gene level
• GO and KEGG annotation and enrichment analysis
Other (please explain)

Poly-A RNA-Seq

Raw Data (standard base-calling & filtering - no further data processing or analysis)
Advanced poly-A RNA-Seq Analysis
• De novo assembly (if applicable)
• Alignment of RNA-Seq reads to customer specified reference genome
• Identification and construction of splice-junctions
• Report of known transcripts with annotation and abundance
• Report of novel transcript with abundance
• Identification of alternate splicing and report of isoform abundance
• Test for differential expression at gene level and transcript level
• GO and KEGG annotation and enrichment analysis
Other (please explain)

Total RNA-Seq

Raw Data (standard base-calling & filtering - no further data processing or analysis)
Advanced Transcriptome Analysis
• De novo assembly (if applicable)
• Alignment of RNA-Seq reads to customer specified reference genome
• Identification and construction of splice-junctions
• Report of known transcripts with annotation and abundance
• Report of novel transcript with abundance
• Identification of alternate splicing and report of isoform abundance
• Test for differential expression at gene level and transcript level
• GO and KEGG annotation and enrichment analysis
• Non-coding RNA information – map to genome, aligment, limited annotation and expression info
Other (please explain)

Data Delivery Preference

Download from our server
Hard drive shipped to you

[PART 4] - Reference Sequence Mapping Information

Genome Sequence Known?

Complete
Partial
None

Web link to sequence files

mRNA (cDNA) Sequence Known

Yes
No

Web link to sequence files

miRNA Sequence Known?

Yes
No

Web link to sequence files

Other Sequence Known (tRNA, rRNA, etc.)?

Yes
No

Web link to sequence files

Custom Sequences?

Yes
No

Web link to sequence files

Basic Research & Discovery

Stress Response Studies

Breeding

DNA Sequencing

RNA Sequencing

Microarray Services

Bioinformatics Services

Featured Customer Publication

Heat stress-responsive transcriptome analysis in heat susceptible and tolerant rice by high-throughput sequencing

The Latest Customer Publications

Search Customer Publications

Featured Poster

Featured News

Degradome Seq Offers New Insight Into The Evolution of MiR827 in Plants

The Latest News

Search News

Weekly Newsletter Signup

About Us

Technologies

VariantPro™ Targeted Sequencing Technology

.

µParaflo® Microarray Technology

United States

China

Phone Us

Email Us

Chat With Us

RNA Sequencing Quote Request

[PART 1] - Project Objectives

[PART 2] - Sample Description

[PART-3] Data Analysis Required

[PART 4] - Reference Sequence Mapping Information

About Us