For each sample, we suggest using at least one, preferably two, small RNA controls from the following list. If you already have small RNA controls of your own preference, please contact us about availability.

Human tissues

  • RNU48
  • U47
  • RNU6B

Mouse tissues

  • snoRNA202
  • snoRNA234

Cell lines

  • RNU24
  • RNU38B
  • Z30

If you want to compare your real-time PCR results with existing microarray expression results, you may want to add one or more microRNAs, which have similar expression levels in all your samples, to your control list.  The following microRNAs have been found relatively stable in human tissues and NCI-60 cell lines.  For microarray expression data from LC Sciences, it is preferable if your control microRNAs have signal intensities above 10,000.

Human tissues

  • has-miR-26b
  • has-miR-92

Cell lines

  • has-miR-16
  • has-miR-423

Application Note from Applied Biosystems – “Endogenous Controls for Real-Time Quantitation of miRNA Using TaqMan® MicroRNA Assays