LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house developed µParaflo® technology platform. We have standard arrays for all mature miRNAs of all species available in the latest version of the miRBase database (Release 21, July 2014). Our service is comprehensive and includes sample labeling, array hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we'll send you both the raw and fully analyzed data.
Featuring µParaflo® Biochip Technology
- A high performance microfluidic custom microarray platform.
- 100% miRBase Version 21 Content
- Complete Content Flexibility (All Species)
- Optimized RNA Hybridization Probes
- Experienced Service Provider (Since 2005) Handling Various Research and Clinical Sample Types – (Including FFPE, Urine and Blood/Plasma Samples)
- Cost Effective 1-Stop Solution
|Comprehensive Service Available||√|
|Microarray Platform||µParaflo®Microfluidic Biochip Technology|
|Starting Sample Material||Total RNA, miRNA, Blood,Cell Pellet, Tissue, FFPE|
|Sample Requirement||1 µg Total RNA (Inquire about lower amounts)|
|Probe Content||miRBase 21 (July 2014)|
|Species Covered||All species for which data exists|
|Probes Tm Equalized||√|
|Add Custom Sequences||√|
|Detection Limit||< 10 attomole|
|Dynamic Range||>3.5 logs|
|Full Data Analysis Included||√|
|Data Delivery Time||2 Weeks|
|Number of Customer Publications to Date||>800|
Our Comprehensive Service
At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection. Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis. We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis. This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.
Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.
In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.
miRBase Version 21 Content Now Available!
June 26th , 2014 – The miRBase sequence database was just updated to version 21. Release 21 of the database contains 28,645 entries representing hairpin precursor miRNAs, expressing 35,828 mature miRNA products, in 206 species. As compared to miRBase version 20(June 2013) a total of 4,196 new hairpin sequences and 5,441 novel mature products have been added.
- This probe content is immediately available on all of our standard or custom microRNA microarrays.
- Flexible content means LC Sciences always delivers the most comprehensive profile of microRNA expression in your sample.
- Read more about this update.
Overview of The Service
Microfluidics Microarray Platform
The µParaflo® technology integrates high throughput parallel synthesis, microfluidics, and digital photolithography to create custom arrays of DNA, RNA, or peptides. Our advanced microarray platform delivers more reliable results than are possible with traditional spotted arrays.
Optimized RNA Hybridization Probes
LC Sciences’ Optimized RNA Hybridization Probes are designed with normalized Tms to ensure uniform hybridization affinity to their microRNA targets under high stringency hybridization conditions enhancing both the sensitivity and specificity of the probes.
The Most Current Content
LC Sciences offers the only microRNA microarray currently available with version 21 probe content. Not 90% or 95% coverage, but 100% of experimentally verified microRNA sequences are represented on all of our arrays.
Complete Content Flexibility
All of LC Sciences’ microarrays are made to order and synthesized to each customer’s particular specifications. Validate predictions, add custom controls, or create totally custom tiling arrays for discovery of novel small-RNAs. The choice is up to you!
No Limitation on Use of Data
Unlike with proprietary probe content, we hold no claims on your data. You have full access and control over the results generated from your experiments.
The Standard for Array Data Quality
The µParaflo® technology enables on-chip synthesis ensuring high probe quality and tight process control. The data obtained by these microarrays is highly reliable and reproducible as validated by qPCR and Northern Blot.
In Depth Data Analysis
LC Sciences’ in-depth data analysis includes all t-Tests, ANOVA and heat maps to get you the information you need. Complex multi-array experiments are no problem for our experienced technical team. We can easily compare samples from different chips or even new experimental data to data from previous experiments.
The Most Customer Publications
LC Sciences’ customers have published over 1000 papers to date; have confirmed that microRNAs play an important role in a wide array of biological functions.