microRNA Sequencing Services
LC Sciences provides a fast, one-stop microRNA sequencing solution – from your sample through data analysis.
Our Comprehensive Services Offer:
- The latest in next-gen sequencing technology
- Experienced staff to assist you with experimental design
- Fast turnaround times
- Full bioinformatics analysis
- Expert technical support to assist with data interpretation
Advanced Data Analysis
Profile microRNA in any species – there is no need for probes based on prior sequence or secondary structure information.
Profiling is transcriptome-wide – investigate all microRNAs, of any size, known and unknown, in your sample.
Achieve digital, quantitative transcript expression analysis – Accurate expression levels over 5 orders of magnitude.
Detect novel and rare transcripts and transcript variants, such as single nucleotide mutations (SNP)
In-Depth Data Analysis Includes:
- Alignment, classification, & functional annotation of all mapped reads
- Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
- Conservation profile of the identified miRNAs analysis
- Pre-miRNA clusters
- miRNA target GO and KEGG enrichment analysis
- Optional miRNA target prediction
Total RNA amount ≥ 2 μg
260/280 >1.8, 260/230 >1.0
RIN value ≥ 7.0
50 bp Single End
10 Million Reads
High Data Quality
Data quality is measured by the percentage of bases with a sequencing quality score above Q30.