microRNA Sequencing Services

LC Sciences provides a fast, one-stop microRNA sequencing solution – from your sample through data analysis.

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Our Comprehensive Services Offer:

  1. The latest in next-gen sequencing technology
  2. Experienced staff to assist you with experimental design
  3. Fast turnaround times
  4. Full bioinformatics analysis
  5. Expert technical support to assist with data interpretation


Library Preparation

Next-Gen Sequencing

Advanced Data Analysis

Any Species

Profile microRNA in any species – there is no need for probes based on prior sequence or secondary structure information.

Transcriptome-wide

Profiling is transcriptome-wide – investigate all microRNAs, of any size, known and unknown, in your sample.

Digital Results

Achieve digital, quantitative transcript expression analysis – Accurate expression levels over 5 orders of magnitude.

Novel Transcripts

Detect novel and rare transcripts and transcript variants, such as single nucleotide mutations (SNP)

In-Depth Data Analysis Includes:

  1. Alignment, classification, & functional annotation of all mapped reads
  2. Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
  3. Conservation profile of the identified miRNAs analysis
  4. Pre-miRNA clusters
  5. miRNA target GO and KEGG enrichment analysis
  6. Optional miRNA target prediction

Sample Data
Sample Data Report

Analysis Pipeline

Sample Requirements

Total RNA amount ≥ 2 μg
260/280 >1.8, 260/230 >1.0
RIN value ≥ 7.0

Recommended
Sequencing Depth

50 bp Single End
10 Million Reads

High Data Quality

Data quality is measured by the percentage of bases with a sequencing quality score above Q30.

Turnkey Solution

These comprehensive services are designed to be one-stop and produce the results needed to quickly advance your biological and biomedical research. For all types of RNA sequencing, our “Total RNA to Data” services include: sample prep/QC, library preparation, RNA sequencing, bioinformatics analysis, and high-level customer support.

Expert Services

LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into RNA research. We don’t merely deliver data; we will handle all the advanced bioinformatics analysis and help you find answers to the questions you’re asking.

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Technical Information

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