Degradome Sequencing    RSQ-1050s

  • Multiplex up to 2 samples per lane
  • High-throughput sequencing (Illumina sequencing by synthesis technology)
  • Single lane 50 cycle run
  • up to 100M raw sequencing reads per lane
  • Raw sequencing data report – either FASTA-A or FASTA-Q files with base-calling quality scores

Degradome Library Preparation    DGD-1002

  • Sample QC
  • Library preparation
  • May include both small RNA and degradome library preparation

In Depth Data Analysis Service    DGD-1007

  • Assessment of sequencing and quality filtering of raw reads
  • Raw sequencing data – up to 100M 50bp single-end reads/lane – either FASTA-A or FASTA-Q files with base-calling quality scores
  • LC Sciences quality filtered data reduced to mappable reads, a list of unique sequences and their copy numbers
  • Identification of rRNAs, tRNAs, snRNAs, snoRNAs, polyN and other non-coding RNAs
  • Distribution of degradation fragments on selected region of genome
  • Identification of target mRNAs
  • Statistical summary of mRNA degradation sites
  • Identification of degradation mRNA related microRNAs from miRBase (Results will be shown in a target plot diagram.)
  • Customer data report – includes a summary of methods and all statistic analysis
  • May include miRNA data analysis if desired
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