Our Comprehensive Service

LC Sciences provides a a one-stop solution (i.e. from sample to data) degradome sequencing service using the Illumina next-generation sequencing technology which enables comprehensive coverage, highly sensitive and specific profiling of miRNAs and mRNA degradome in your total RNA sample.

This service is comprehensive – from sample to data, providing advanced technology and years of experience in efficient discovery and profiling of RNAs.

Our comprehensive profiling service includes:

  • Sample QC
  • Library preparation (includes both small RNA and degradome library prep)
  • High-throughput sequencing (RNA-Seq method, Illumina Next-Gen sequencing technology)
  • Bioinformatics analysis
  • Customer data report

Data Analysis / Results

For each sample analyzed, the customer receives:

  • Assessment of sequencing and quality filtering of raw reads
  • Raw sequencing data – up to 100M  50bp single-end reads/lane – either FASTA-A or FASTA-Q files with base-calling quality scores
  • LC Sciences quality filtered data reduced to mappable reads, a list of unique sequences and their copy numbers
  • Identification of rRNAs, tRNAs, snRNAs, snoRNAs, polyN and other non-coding RNAs
  • Distribution of degradation fragments on selected region of genome
  • Identification of target mRNAs
  • Statistical summary of mRNA degradation sites
  • Identification of degradation mRNA related microRNAs from miRBase (Results will be shown in a target plot diagram.)
  • Customer data report – includes a summary of methods and all statistic analysis
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