Digital Gene Expression – (mRNA) Sequencing    RSQ-1050s

  • Multiplex up to 10 samples per lane
  • High-throughput sequencing (Illumina sequencing by synthesis technology)
  • Single lane 50 cycle run
  • up to 100M raw sequencing reads per lane
  • Raw sequencing data report – either FASTA-A or FASTA-Q files with base-calling quality scores

Digital Gene Expression – (mRNA) Library Preparation    GES-1002

  • Sample QC
  • Small RNA selection
  • Library preparation
  • Single molecule clonal amplification

Digital Gene Expression – Bioinformatics Service    GES-1007

  • Assessment of sequencing and quality filtering of raw reads
  • Raw sequencing data – up to 100M 50bp single-end reads/lane – either FASTA-A or FASTA-Q files with base-calling quality scores
  • LC Sciences quality filtered data reduced to mappable reads, a list of unique sequences and their copy numbers
  • Genomic mapping of quality sequencing reads
  • Test for differential expression at gene level and transcript level
  • GO and KEGG annotation and enrichment analysis
  • Customer data report – either FASTA-A or FASTA-Q files – includes raw data, quality filtered data, analyzed data, includes a summary of methods and all statistical analysis
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