Bioinformatics Services:

We can accept raw data from the following next-gen sequencing platforms:

  • Illumina
  • Ion Torrent
  • 454
  • SOLiD

We can deliver fully analysis datasets in 3-5 business days.

Your data can be transferred to us via a secure ftp server.

Small RNA Sequencing Data Analysis Services include:

  • Mapping to reference sequence
  • Includes custom construction of reference database(s) for mapping – miRBase, genome, etc
  • Sequence data can be aligned to any publicly available small RNA databases for annotation of known small RNA
  • Unknown small RNAs can be aligned with reference genomes
  • Alignment, classification, & functional annotation of all mapped reads
  • Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
  • Conservation profile of the identified miRNAs analysis
  • Pre-miRNA clusters
  • miRNA target prediction (optional)
  • miRNA target GO and KEGG enrichment analysis
  • Customer data report – includes a summary of methods and all statistic analysis
  • Sample Data Report

Degradome Sequencing Data Analysis Services include:

  • Identification of rRNAs, tRNAs, snRNAs, snoRNAs, polyN and other non-coding RNAs
  • Distribution of degradation fragments on selected region of genome
  • Identification of target mRNAs
  • Statistical summary of mRNA degradation sites
  • Identification of degradation mRNA related microRNAs from miRBase (Results will be shown in a target plot diagram.)
  • Customer data report – includes a summary of methods and all statistic analysis
  • May include miRNA data analysis if desired

Digital Gene Expression Sequencing Data Analysis Services include:

  • Genomic mapping of quality sequencing reads
  • Test for differential expression at gene level and transcript level
  • GO and KEGG annotation and enrichment analysis
  • Customer data report – either FASTA-A or FASTA-Q files – includes raw data, quality filtered data, analyzed data, includes a summary of methods and all statistical analysis
  • Sample Data Report

RNA-Seq (mRNA)  Sequencing Data Analysis Services include:

  • Processed data is reduced to mappable reads
  • De Novo Assembly (if applicable)
  • Alignment of RNA-Seq reads to customer specified reference genome
  • Identification and construction of splice-junctions
  • Report of known transcripts with annotation and abundance
  • Report of novel transcript with abundance
  • Identification of alternate splicing and report of isoform abundance
  • Test for differential expression at gene level and transcript level
  • GO and KEGG annotation and enrichment analysis
  • Customer data report – includes a summary of methods and all statistical analysis
  • Sample Data Report

Total RNA-Seq (Whole Transcriptome)  Sequencing Data Analysis Services include:

  • Processed data is reduced to mappable reads
  • De Novo Assembly (if applicable)
  • Alignment of RNA-Seq reads to customer specified reference genome
  • Identification and construction of splice-junctions
  • Report of known transcripts with annotation and abundance
  • Report of novel transcript with abundance
  • Identification of alternate splicing and report of isoform abundance
  • Test for differential expression at gene level and transcript level
  • GO and KEGG annotation and enrichment analysis
  • Non-coding RNA information – map to genome, alignment, limited annotation and expression info
  • Customer data report – includes a summary of methods and all statistical analysis

Additional custom bioinformatics analysis – If you have additional, more complex bioinformatics needs such as functional gene information mining or comparative genomic analysis, please contact us to learn how we can help get you the results you need to keep your research moving ahead.

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