Plant miRNAs have been shown to be involved in various biotic and abiotic stress responses

Plant miRNAs have been shown to be involved in various biotic (bacterial and viral pathogenesis) and abiotic stress responses such as oxidative, mineral nutrient deficiency, drought, salinity, temperature, cold (chilling), and other abiotic stress. miRNA expression profiling reveals that miRNAs which are involved in the progression of plant growth and development are differentially expressed during abiotic stress responses. The high-throughout techniques can provide genome-wide identification of stress-associated miRNAs under various abiotic stresses in plants.

miRNA microarray can monitor the expression pattern of thousands of genes simultaneously to determine microRNA profiling in stress-induced plant tissues/cells. The differentially expressed miRNAs further could be validated by quantitative real-time PCR (qRT-PCR) or by northern blot analysis. RNA-Seq technologies have become very powerful tools in the identification and quantification of small RNAs involved in gene regulation in different types of responses in plants and animals and degradome sequencing has revealed many novel plant miRNA targets1.

LC Sciences offers a portfolio of services to facilitate stress response studies in agricultural genomics. Contact us to find out more.

Poly(A) RNA-Seq Sequencing Services

RNA-Seq technology is providing fresh biological insight into the transcriptome. At its most powerful, it can be used to determine the structure of genes, their splicing patterns …

microRNA Profiling Services

microRNAs are small noncoding RNA molecules that function broadly as negative regulators of gene expression to control a wide range of cellular processes. A genome-wide microRNA (miRNA) expression profiling service…

Degradome Sequencing Services

Degradome sequencing (Degradome-Seq), also referred to as parallel analysis of RNA ends (PARE), a modified 5′-rapid amplification of cDNA ends…

16S rRNA Gene Sequencing
16S rRNA gene sequencing is commonly used for identification, classification and quantitation of microbes within complex biological mixture such as environmental samples (ex marine water) and gut samples…


1Kumar R. (2014) Role of MicroRNAs in Biotic and Abiotic Stress Responses in Crop Plants. Appl Biochem Biotechnol 174(1), 93-115 [abstract]