VariantPro™ Targeted Sequencing Technology
A novel multiplex PCR technology that combines two major (patent pending) innovations to facilitate simple operation and high performance in the preparation of targeted sequencing libraries
One innovation in the VariantPro system is Relay-PCR, in which one pair of common (indexed library) primers and multiple pairs of specific primers are mixed with a genomic DNA sample in a single tube. A single PCR run seamlessly combines two functionally separated reaction phases, namely target replication and library amplification. This results in a significantly simplified work flow. Another innovation in the VariantPro system is Omega-Primer which is composed of three functional sections: a 5p arm designed to ensure stable binding to a corresponding template, a 3p arm designed to double check sequence specificity and initiate polymerase extension, and a separation segment between the two arms. The use of two separate binding sections provides primer design freedoms that balance priming specificity and binding strength.
µParaflo® Microarray Technology
Massively parallel oligonucleotide and peptide synthesis on a microchip based on the µParaflo® microfluidics technology
Our scientists and engineers have developed platform technologies that encompass a new class of microfluidic µParaflo® reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers.