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LC Sciences - Technologies for Genomics and Proteomics Discoveries : LC Sciences offers specialty microarray and sequencing services for nucleic acid/protein profiling and functional analysis, biomarker-discovery, novel drug screening, and the custom development of miniaturized assay devices for diagnostics and biosensing applications. Our innovative products and comprehensive services are based on the µParaflo® parallel-synthesis technology and offer unprecedented flexibility and customization capability.
LC Sciences - Technologies for Genomics and Proteomics Discoveries : LC Sciences offers specialty microarray and sequencing services for nucleic acid/protein profiling and functional analysis, biomarker-discovery, novel drug screening, and the custom development of miniaturized assay devices for diagnostics and biosensing applications. Our innovative products and comprehensive services are based on the µParaflo® parallel-synthesis technology and offer unprecedented flexibility and customization capability.
LC Sciences - Search the LC Sciences Website : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Search the LC Sciences Website
LC Sciences - Contact Information : LC Sciences Contact Information
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Products : Our genomics and proteomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. Our comprehensive services includes sequence retrieval from public databases and literature, and/or assistance with your sequence designs, nucleic acid hybridization, protein binding or enzymatic assays using samples provided by you, single or dual color detection of the hybridization using a suitable method, image data processing, and signal data processing.
LC Sciences - Ordering Information : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. LC Sciences products may be purchased worldwide directly through our United States office. Customers outside the United States may also order though one of our other offices or through a local distributor for your area. Most orders can be placed simply by emailing us your order form (orders@lcsciences.com) or, for services, just shipping your samples to us along with the required sample submission forms.
LC Sciences - uParaflo Microfluidic Technology - Overview : The scientists and engineers at our technology partner, Atactic Technologies, have developed platform technologies that encompass a new class of microfluidic µParafloTM reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers.
LC Sciences - Support Information - Publications : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Technology and Customer Publications Page
LC Sciences - Company News & Events : February, 2009 - microRNA / small RNA Sequencing Services Launched - Now a one-stop solution for all your small RNA discovery, detection and expression profiling needs. January, 2009 - Targeted Sequencing - Sample Enrichment Service Available - Take advantage of high-throughput sequencing technologies with target-specific selection of defined sequences of your interest. Genomic DNA, PCR product, or RNA accepted. Sept 1, 2008 - The Sanger Institute announces the largest single update in the history of miRBase with the of 2062 new mature miRNA sequences. In addition to sequence updates and additions to species included in previous miRBase versions, mature miRNA sequences from many new species are included for the first time.
LC Sciences - Company : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Our products are based on platform technologies that encompass advanced digital chemical synthesis, picoliter scale biochemical analysis, and massive parallel microfluidic reaction devices. This µParaflo® technology enables us to provide unique and customizable oligonucleotide and peptide microarray products designed for nucleic acid and protein-profiling, biomarker-screening, drug screening, and development of diagnostic-devices.
LC Sciences - microRNA Detection Microarray Service - Overview : LC Sciences provides a genome-wide microRNA (miRNA) expression profiling service using µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs. We have standard arrays for mature miRNA of all species available in the latest version of the Sanger miRBase database (Release 14.0). Our service is comprehensive and includes small RNA enrichment from your total RNA sample, single or dual color labeling, hybridization, image data processing and data analysis. Two-three weeks after receiving your total RNA samples, we send you the analyzed data, representative and original images of the array, and raw data. We offer additional products and services including arrays containing miRNA*, walk/tiling arrays, custom arrays (first 100 probes free of charge), and in-depth analysis for multiple data sets. The in-depth data analysis may include multi-array normalization, t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.
LC Sciences - microRNA Microarray Service - Product News - Oct 8, 2009 - Sanger miRBase microRNA Sequence Database Updated to Release 14.0 : October 8th, 2009 - LC Sciences now has standard arrays available for all species listed in the newly created Plant MicroRNA Database (PMRD). The plant miRNA database (PMRD) integrates available plant miRNA data deposited in public databases, gleaned from the recent literature, and data generated by the database organizers. This database contains sequence information, secondary structure, target genes, expression profiles and a genome browser. In total, there are 8433 miRNAs collected from 121 plant species in PMRD, including model plants and major crops such as Arabidopsis, rice, wheat, soybean, maize, sorghum, barley, etc.
LC Sciences - microRNA Microarray Service - Product News - Sept 15, 2009 - Sanger miRBase microRNA Sequence Database Updated to Release 14.0 : September 15th, 2009 - miRBase microRNA Sequence Database Updated to Release 14.0 - The latest version (14.0) of the miRBase sequence database for known miRNAs was released today (http://www.mirbase.org). The miRBase database has moved to a new location at http://www.mirbase.org/, hosted in the Faculty of Life Sciences, University of Manchester. miRBase in Manchester is now funded by the BBSRC. The miRBase Targets site and pipeline have devolved into the Enright research group at EBI under the name MicroCosm (http://www.ebi.ac.uk/enright-srv/microcosm/htdocs/targets/). All pre-existing Sanger site URLs should forward to their new locations. Please update your links, and note the new contact email address (mirbase@manchester.ac.uk). miRBase 14 is now available at http://www.mirbase.org/. Please see the README for the new release at ftp://mirbase.org/pub/mirbase/CURRENT/README.
LC Sciences - Company Press Release September 4th, 2009 : LC Sciences announced today the publication of the 100th peer-reviewed study by one of its customers using the company’s microarray service for analyzing microRNA (miRNA) expression profiles and for discovery of novel small RNAs. These studies, by leading researchers in the field, contribute to a fast growing body of knowledge defining this recently discovered class of regulatory RNAs.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Genomics : Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. Our comprehensive services includes sequence retrieval from public databases and literature, and/or assistance with your sequence designs, nucleic acid hybridization assays using samples provided by you, single or dual color detection of the hybridization using a suitable method, image data processing, and signal data processing.
LC Sciences - microRNA Detection Microarray Service - Customer Publications : A list of customer publications and links - Our customers publications cover many areas of research including Small RNA Discovery, Cancer Research, Neuroscience, Cardiovascular Research, Small RNA Mechanism of Action, Reproductive Biology, Plant Science, Virology, Stem Cell Research, and Endocrinology
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Proteomics : Our proteomics products and services include the use of custom synthesized peptide microarrays based on the µParaflo®on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. Our comprehensive services includes sequence retrieval from public databases and literature, and/or assistance with your sequence designs, binding and enzymatic assays using protein kinases, antibodies, or other proteins provided by you, single or dual color detection of the binding using a suitable method, image data processing, and signal data processing. LC Sciences offers additional µParaflo® technology derived proteomics products that serve needs for construction of biomolecular libraries containing up to millions of different biomolecules of defined sequences.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Sitemap : LC Sciences Sitemap - This is a list of all the content from the official LC Sciences Website - Homepage - Ordering - Technology - Publications - News - Company
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LC Sciences - Technologies for Genomics and Proteomics Discoveries : LC Sciences offers specialty microarray and sequencing services for nucleic acid/protein profiling and functional analysis, biomarker-discovery, novel drug screening, and the custom development of miniaturized assay devices for diagnostics and biosensing applications. Our innovative products and comprehensive services are based on the µParaflo® parallel-synthesis technology and offer unprecedented flexibility and customization capability.
LC Sciences - microRNA Sequencing Service - Overview : LC Sciences now provides a one-stop solution for all your microRNA (miRNA) discovery and expression profiling needs. High-throughput sequencing is now available in addition to existing microarray and qRT-PCR profiling services for the most complete picture of microRNA expression in your samples. microRNA sequencing is a new method and a powerful tool to identify and quantitatively decode the entire population of microRNAs in your sample.
LC Sciences - microRNA qRT-PCR Service - Overview : LC Sciences now provides a one-stop solution for all your microRNA (miRNA) expression profiling needs. We can provide you with both microarray and qRT-PCR profiling services for the most complete picture of microRNA expression in your samples. While microarrays provide efficient genome-wide screening of miRNA expression, qRT-PCR is the gold standard for validation of microarray expression data and provides quantitative analysis of microRNA expression in real time which can help you to zero in on the miRs of interest in your samples revealed by microarrays. In addition, the specificity of real time PCR expression data can provide further understanding of the true biological significance of these miRs.
LC Sciences - Targeted Sequencing - Sample Enrichment Service - Overview : LC Sciences’ µParaflo® technology is particularly well suited to provide sequence capture solutions through massively parallel synthesis of high quality DNA on biochips. Thousands of customer specified oligonucleotide sequences are in situ synthesized on a programmable high density microfluidics chip. Standard oligo synthesis chemistry and advance microfluidics design ensure high quality synthesis. Genomics samples can be applied directly to the biochip via microfluidics for on-chip sequence capture. Enriched targeted regions are recovered ready for sequencing.
LC Sciences - Small Non-coding RNA Discovery Microarray Service - Overview : Non-coding (ncRNAs) are a class of RNAs that do not encode proteins but instead possess regulatory function at the level of RNA in the cell. Custom microarrays have been shown to be a effective experimental approach to discovery of novel ncRNAs as well as a method to experimentally test computationally predicted ncRNAs. LC Sciences provides a small non-coding RNA discovery service using innovative µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of small RNAs in your sample. This service is comprehensive; from array design to sample to data, allowing the most efficient novel discovery of small regulatory/functional RNA embedded within non-coding RNAs (ncRNAs) or genomic RNAs.
LC Sciences - Small RNA Discovery Sequencing Service - Overview : Profile small RNA in any species - there is no need for probes based on prior sequence or secondary structure information. Profiling is transcriptome-wide - investigate all small RNAs, of any size, known and unknown, in your sample. Achieve digital transcript expression analysis - Accurate quantitation of expression levels over 5 orders of magnitude of transcript abundance. Detect rare transcripts and transcript variants, such as single nucleotide mutations (SNP).
LC Sciences - Gene Expression Analysis Service - Overview : LC Sciences provides access to Gene Expression Analysis Service through our partnership with SeqWright DNA Technology Services. SeqWright is an Affymetrix® Authorized Service Provider with over 30 years of combined experience involving microarray technologies used in both industry and diagnostic environments. SeqWright's Affymetrix® service is GLP-compliant and CLIA certified for handling patient samples.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Overview : OligoMix® is a product developed in response to the need for pooled oligonucleotides used in multiplexing reactions. We synthesize thousands of oligonucleotide sequences at once in parallel and then cleave the oligos releasing them into solution in a single microtube. Synthesis occurs via standard DMT chemistry assuring efficient stepwise yield and high quality final product. The product is delivered as a pool in a single microtube - ready to use for your experiment.
LC Sciences - DNA/RNA Aptamer Microarray Service - Overview : LC Sciences provides unique aptamer microarray services using a novel µParaflo® technology, aptamer sequence databases, and sequence design software to suit your needs for basic research and screening of aptamers as drug candidates. This comprehensive service includes assistance with sequence design, synthesis of standard or custom DNA/RNA (2’-OMe) microarrays, binding assays using proteins, nucleic acids, carbohydrates, or other kinds of small molecules provided by you, single or dual color detection of the binding using a suitable method, image data processing, and signal data processing. Two-four weeks after receiving your sample(s), we send you the analyzed data, representative and original images of the array, and raw data. We offer additional services for in-depth binding data analysis and curve analysis of quantitative measurements.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Overview : Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. Our comprehensive services includes sequence retrieval from public databases and literature, and/or assistance with your sequence designs, nucleic acid hybridization assays using samples provided by you, single or dual color detection of the hybridization using a suitable method, image data processing, and signal data processing.
LC Sciences - Kinase Profiling Microarray Service - Overview : LC Sciences provides a comprehensive kinase analysis service utilizing high density protein kinase substrate (PKS) peptide microarrays synthesized on µParaflo® microfluidic chips for proteomic scale kinase profiling, quantitative measurement of kinase kinetic activities, and drug discovery research. This comprehensive service includes preparation of your kinase sample, single or dual color enzymatic reactions, array scanning and data analysis. Two-three weeks after receiving your kinase sample(s), we send you a data summary report containing background subtraction, control and reference signal guided data processing, list of detected signals, and data averaging. We offer additional services for in-depth kinetic data analysis and curve analysis of quantitative measurements.
LC Sciences - Epitope Mapping Peptide Microarray Service - Overview : LC Sciences now offers a comprehensive epitope mapping service for high throughput, high-resolution identification of epitopes and other protein-protein interactions. Through the use of overlapping peptides as epitopes on a custom synthesized addressable peptide microarray, we can systematically screen thousands of sequences in a single experiment. A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events. This combination of high-throughput capacity with quantitative measurement enables us to quickly and efficiently identify high affinity and high specificity target binding compounds. Epitope mapping assays are applicable for immunological studies, vaccine development, and biosensor development.
LC Sciences - Phosphopeptide Binding Peptide Microarray Service : Phosphoprotein binding proteins (PPBP) contain domains which binds to phosphorylation sites in phosphoprotein or phosphopeptide (PPEP). LC Sciences provides a straightforward, high-throughput method for obtaining quantitative measurements of phosphoprotein activities. Through the use of high density arrays (PepArray™) with addressable “signature” peptides for specific kinds of protein binding domains we can systematically screen thousands of sequences in a single experiment. A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events.
LC Sciences - Protein Binding Microarray Service - Overview : LC Sciences now offers customizable parallel synthesis and microfluidic technologies to create a microarray of thousands of peptides for binding to your protein or antibody sample and a comprehensive protein and/or antibody analysis service utilizing these high density peptide microarrays for high throughput detection, concentration titration, and screening applications. This combination of high-throughput capacity with quantitative measurement, enables us to quickly and efficiently identify high affinity and high specificity target binding compounds. These quantitative assays are applicable for immunological studies, vaccine development, and biosensor development.
LC Sciences - Protein - Aptamer Binding Microarray Service - Overview : LC Sciences provides unique aptamer microarray services using a novel µParaflo® technology, aptamer sequence databases, and sequence design software to suit your needs for basic research and screening of aptamers as drug candidates. This comprehensive service includes assistance with sequence design, synthesis of standard or custom DNA/RNA (2’-OMe) microarrays, binding assays using proteins, nucleic acids, carbohydrates, or other kinds of small molecules provided by you, single or dual color detection of the binding using a suitable method, image data processing, and signal data processing. Two-four weeks after receiving your sample(s), we send you the analyzed data, representative and original images of the array, and raw data. We offer additional services for in-depth binding data analysis and curve analysis of quantitative measurements.
LC Sciences - Peptide Microarray Service - PepArray Technology : Custom high density peptide synthesis and multiplex proteomics assays on a microchip based on the µParaflo® microfluidics technology. PepArray technology involves in situ (on chip) high density peptide synthesis and multiplex protein assays carried out in a microfluidic picoliter scale microarray. The PepArrayÔ is an assay reaction device which allows thousands of titration reactions, multiple step enzymatic reactions, and other biochemical reactions normally carried out in individual micro tubes or microtiter plates.
LC Sciences - Peptide Microarray Service - Picoliter Scale Titer Plates : PepArrayÔ technology involves in situ (on chip) high density peptide synthesis and multiplex protein assays carried out in a microfluidic picoliter scale microarray. These are custom synthesized peptide microarrays (see PepArrayÔ technology) containing 4K-30K features. Each feature is actually a miniature (270 pl) reaction chamber. We do not spot pre-synthesized peptides onto the array; instead, each peptide is synthesized in parallel in an individual reaction chamber. Not only can we synthesize a unique sequence in each chamber but our unique synthesis chemistry also makes it possible for us to vary the density of peptide synthesized in each chamber. Repeating sequences of the same peptide can be synthesized at different molecular densities and thus are presented at different “concentrations” creating a gradient across the array.
LC Sciences - Distributors : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. We have distributors worldwide. Contact information is listed here.
LC Sciences - microRNA Detection Microarray Service - Ordering : LC Sciences - microRNA Detection Microarray Service - Ordering - Sample Preparation - Sample Amount Required - RNA Extraction - Quality Control of RNA Sample - Placing Your Order - Complete a Sample Submission Form - Packaging and Shipping
LC Sciences - microRNA qRT-PCR Service - Ordering : qRT-PCR Total RNA Sample Preparation - Sample Amount Required - RNA Extraction - Quality Control of RNA Sample - Placing Your Order - Complete a Sample Submission Form - Packaging and Shipping
LC Sciences - Small Non-Coding RNA Discovery Microarray Service - Ordering : Small Non-Coding RNA Discovery Microarray Service - Ordering - Total RNA Sample Preparation - Sample Amount Required - RNA Extraction - Quality Control of RNA Sample - Placing Your Order - Determine Array Content and Select Services - Complete a Sample Submission Form - Packaging and Shipping
LC Sciences - Gene Expression Analysis Service - Ordering : Sample Preparation - Sample Amount Required - RNA Extraction - Quality Control of RNA Sample - Placing Your Order - Complete a Sample Submission Form - Packaging and Shipping
LC Sciences - OligoMix® - Oligonucleotide Mixture - Ordering : Download the OligoMix® Order Form. Save the form to a directory on your computer. Fill in your contact information on the top of the Order Form worksheet. Click the Add Sequences next to Tube 1. Enter your oligo sequences (you can copy/paste) on this worksheet. When you have finished adding sequences, click the Go To Order Info button to switch back to the first worksheet. If the oligos will be modified in any way, enter the code in the Modification Code column. Enter the number of tubes to synthesize in the # of Tubes column. (the default is 1 tube) Repeat steps 4-7 for any additional tubes to be synthesized. The Order File automatically calculates the total cost. Save the Order File. Attach this order file to an email and send to us at: sales@lcsciences.com.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Ordering : Our specialty genomics microarrays are custom products that can be used as part of a comprehensive project for biomolecule analysis. As these projects are often complex in nature, we ask that you please download and complete a project description form so that we may better understand your objectives for the experiment(s). A customer service representative will contact you within 24 hours to discuss the specifics of your project and give you an idea of the cost.
LC Sciences - Kinase Profiling Microarray Service - Ordering : Please follow the steps outlined below to place your order for a Kinase Microarray Profiling Service. Define the details of your experiments. Download a copy of our Project Description Form. Execute a service agreement. Complete a sample submission form prior to sending your sample to us. Send us your sample - Please see our protein sample submission page for sample requirements and instructions for shipping us your sample.
LC Sciences - Protein Binding Microarray Service - Ordering : Please follow the four steps outlined below to place your order for a Protein Binding Microarray Profiling Service. Define the details of your experiments. Download a copy of our Project Description Form. Execute a service agreement. Complete a sample submission form prior to sending your sample to us. Send us your sample - Please see our protein sample submission page for sample requirements and instructions for shipping us your sample.
LC Sciences - Protein - Aptamer Binding Microarray Service - Ordering : Please follow the four steps outlined below to place your order for a DNA/RNA Aptamer Binding Microarray Service. Define the details of your experiments. Download a copy of our Project Description Form. Complete as much of this form as possible. We will use the information you provide to design your experiments. Note - for items left blank we will insert information based on our successful experiences. Email the form to orders@lcsciences.com. Customer service will contact you to finalize the details of your project. Execute a service agreement. When the details have been finalized we will send you a service agreement and a quote. Sign the service agreement and FAX it back to us. (713) 664-8181. Use the quote to obtain a purchase order from your institution. Complete a sample submission form prior to sending your sample to us. In order to ensure a successful experiment, we need to know as much about your sample as you can tell us. The form can be emailed to us at: orders@lcsciences.com beforehand or sent in the package along with your sample. Note - If you send the form in the package with samples please place it in a separate waterproof bag. Send us your sample
LC Sciences - Phosphopeptide Binding Peptide Microarray Service - Ordering Instructions : Please follow the four steps outlined below to place your order for Phosphopeptide Binding - Peptide Microarray Service - Describe the details of your experiment(s) - Complete a service agreement - Complete a sample submission form - Send us your sample
LC Sciences - uParaflo Microfluidic Technology - Technology Animation : PicoArray Reactor - The microfluidic PicoArray reactor is made from silicon using standard microelectronic fabrication procedures. The reactor contains three topographical features: pico-reaction chambers, fluid microchannels, and inlet/outlet through holes. The fluid microchannels are of a tapered shape that was derived from a fluid mechanical model to produce a uniform flow rate across all reaction chambers. This technology enables a high density of uniform spots.
LC Sciences - uParaflo Microfluidic Technology - Publications : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Technology Publications - µParaflo® in situ Oligonucleotide Microarray Synthesis Technology Articles - µParaflo® in situ Peptide Microarray Synthesis Technology Articles - OligoMix® Massive Parallel Synthesis Technology Articles
LC Sciences - uParaflo Microfluidic Technology - FAQs :
LC Sciences - uParaflo Microfluidic Technology - Technical Bulletins : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Technical Bulletins - µParaflo® Microfluidics Synthesis Technology - Massively Parallel Oligonucleotide Synthesis on a Microchip based on the µParaflo® Microfluidics Technology - Massively Parallel Peptide Synthesis on a Microchip based on the µParaflo® Microfluidics Technology
LC Sciences - Newsletter Signup : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Signup to receive e-newsletters featuring information related to your areas of interest.
LC Sciences - uParaflo Microfluidic Technology - Data :
LC Sciences - uParaflo Microfluidic Technology - Spotted array detail : The scientists and engineers at our technology partner, Atactic Technologies, have developed platform technologies that encompass a new class of microfluidic µParafloTM reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers.
LC Sciences - uParaflo Microfluidic Technology - uParaflo microarray detail : The scientists and engineers at our technology partner, Atactic Technologies, have developed platform technologies that encompass a new class of microfluidic µParafloTM reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Publications : OligoMix® Application Publications - siRNA Target Library Preparation - Protein-Nucleic Acid Interaction Studies - Preparation of Synthetic DNA Constructs - Gene Synthesis - OligoMix® Review Publications - OligoMix® Synthesis Technology Publications
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Publications : Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. In addition, the flexibility of µParaflo® enables the incorporation of modifications within the probes, which significantly expands the range of applications for these microarrays. Listed below are publications illustrating the application of custom microarrays containing non-standard probes. Protein-Oligonucleotide Binding (Transcription Factor) Microarrays - Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain.
LC Sciences - Company Press Release October 14th, 2009 : LC Sciences announced today the publication of the 100th peer-reviewed study by one of its customers using the company’s microarray service for analyzing microRNA (miRNA) expression profiles and for discovery of novel small RNAs. These studies, by leading researchers in the field, contribute to a fast growing body of knowledge defining this recently discovered class of regulatory RNAs.
LC Sciences - Company Press Release February 26th, 2009 : LC Sciences has announced the availability of a custom target enrichment service for next-generation sequencing applications. LC Sciences now provides a service for Target-Specific Selection of a defined genomic region (such as Mbp region at a specific location, suspected cancer regions, SNP regions, regions for genomic comparisons) or RNA sequences (such as sets of transcriptome sequences known from previous screening experiments, mRNAs, and miRNAs). This new service enables researchers to take full advantage of new “next-generation” high throughput sequencing technologies.
LC Sciences - Company Press Release September 17th, 2008 : Recently, researchers at Baylor College of Medicine employed a discovery strategy of computational prediction and microarray expression profiling to uncover a large number of novel small RNAs in embryonic stem (ES) cells3. Dr. Preethi Gunaratne and co-workers reported using computation, custom small RNA microarray from LC Sciences, and validation experiments to uncover hundreds of novel small RNAs in ES cells. These small non-coding RNAs (ncRNAs) were from adult tissue progenitor transcript sequences and are micro-conserved elements. The detection probes were designed for custom array synthesis by LC Sciences, Houston, TX (www.lcsciences.com) and included the standard microRNA (miRNA) sequences in miRBase4, a comprehensive panel of 2,617 micro-conserved ncRNA sequences, and several hundreds of literature reported small RNAs or miRNAs. Two generations of custom array designs were used for optimizing the probes. These profiling studies using ES cell lines as well as normal adult mouse tissues identified 545 small RNAs that are enriched in ES cells over adult cells. Of these, more than 100 exhibited down-regulation in ES gene-knockout cells typical of miRNA.
LC Sciences - Company Press Release May 6th, 2008 : Houston, TX – May 6, 2008 – LC Sciences announced today that it has entered into a strategic marketing alliance with DNAVision to increase accessibility to its custom microRNA profiling service and enhance both its customer and technical service to researchers in Europe. LC Sciences’ microRNA profiling service is powered by µParaflo® microfluidic technology and makes use of flexible custom microarrays for expression profiling as well as discovery of novel small RNAs and validation of predicted microRNAs. DNAVision, specialized in molecular biology, provides a wide range of genetic services and products to the pharmaceutical, food, and biotechnology industries.
LC Sciences - Company Press Release February 27th, 2008 : Houston, TX – February 27, 2008 – LC Sciences announced today the publication of the 30th peer-reviewed study by one of its customers using the company’s microarray service for analyzing microRNA expression profiles and for discovery of novel small RNAs. These studies, by leading researchers in the field, contribute to a fast growing body of knowledge defining this recently discovered class of regulatory RNAs.
LC Sciences - Company Press Release August 9th, 2007 : Houston, TX – August 9, 2007 – Taking advantage of its flexible µParaflo®Chip Technology, Houston based LC Sciences today announced immediate availability of probe content miRBase 10.0 for their microRNA (miRNA) microarray customers. This announcement comes less than a week after Sanger Institute’s update of their sequence database for known miRNAs (miRBase)1 to version 10.0 (http://microrna.sanger.ac.uk/sequences/). As the jump in version numbers from 9.2 to 10.0 suggests, this update marks a major milestone and features significant changes: 489 new hairpin sequences and 971 novel mature miR and miR* experimentally verified products have been added.
LC Sciences - Company Press Release May 9, 2006 : Houston, TX – May 9, 2006 – LC Sciences announced today that the probe content of its line of standard and custom microRNA microarrays used in their comprehensive expression profiling service would keep pace with the latest version (Release 8.1) of the Sanger miRBase sequence database and that microarrays containing the new sequences are already available. The Sanger Institute released their latest version of their miRBase sequence database for known microRNAs on Friday May 5, 2006.
LC Sciences Blog :
LC Sciences Blog :
LC Sciences - Company News & Events 2009 : January 11 - 16, 2009 - San Diego, CA - PepTalk - The Definitive Protein Focused Event - We invite you to visit us at booth #13 to learn more about our custom peptide microarray products and services for kinase substrate profiling, epitope mapping, and phosphopeptide-binding protein studies. Dr. Christoph Eicken (Head of Technical Services, Microarrays) will be presenting a poster: Proteomic Kinase Substrate and Phosphopeptide Chip Technology for Protein Profiling - Although a number of proteomics technologies are well-developed, the demand for reliable, sensitive, accurate, and comprehensive measurements of protein expression remains unfulfilled. Our work is based on using a) protein kinase (PK) phosphorylation activities and b) phosphopeptide-phosphoprotein binding domain (PPEP-PPBD) interactions to detect protein in complex biological samples. We have a novel, in situ, on-microchip synthesis technology (µParaflo®) that allows us to construct in situ synthesis of high-density peptide arrays according to custom designs. We also apply this flexible peptide array technology for rapid and reliable quantitative measurements for phosphorylation and protein binding – 41 96-well titer plate experiments (i.e. conventional experiments) can be accomplished simultaneously on one chip. Our ultimate goal is to provide new, powerful research and clinical study tools to address the critical needs in proteomic research, clinical diagnosis, and therapeutic treatment.
LC Sciences - microRNA Microarray Service - Product News - March 2009 - Sanger miRBase microRNA Sequence Database Updated to Release 13.0 : March 9, 2009 - Sanger miRBase microRNA Sequence Database Updated to Release 13.0 - The Sanger Institute released the latest version (13.0) of their miRBase sequence database for known miRNAs today ( http://microrna.sanger.ac.uk/sequences/) The number of verified microRNAs has been increased from 8619 to 9539 hairpin precursor miRNAs expressing 9169 mature miRNA products. (miRBase release summary). With the addition of 1004 new mature miRNA products, the Sanger microRNA Sequence Database (miRBase) has announced a significant update.
LC Sciences - microRNA Microarray Service - Product News - Sept 1, 2008 - Sanger miRBase microRNA Sequence Database Updated to Release 12.0 : Sept 1, 2008 - Sanger miRBase microRNA Sequence Database Updated to Release 12.0 - The miRBase sequence database1 is a comprehensive database of miRNA sequence data, annotation, and predicted gene targets and is the primary public repository for these data miRBase also provides a gene-naming service for assigning official miRNA names to novel miRNAs before they are published. miRBase provides a convenient, searchable online database, allowing the user to search by key word or sequence for information about known miRNAs and their targets and is freely available to all at http://microrna.sanger.ac.uk/.
LC Sciences - microRNA Microarray Service - Product News - April 16, 2008 - Sanger miRBase microRNA Sequence Database Updated to Release 11.0 : April 16, 2008 - Sanger miRBase microRNA Sequence Database Updated to Release 11.0 - The miRBase sequence database1 is a comprehensive database of miRNA sequence data, annotation, and predicted gene targets and is the primary public repository for these data miRBase also provides a gene-naming service for assigning official miRNA names to novel miRNAs before they are published. miRBase provides a convenient, searchable online database, allowing the user to search by key word or sequence for information about known miRNAs and their targets and is freely available to all at http://microrna.sanger.ac.uk/.
LC Sciences - microRNA Microarray Service - Product News - December 19, 2007 - Sanger miRBase microRNA Sequence Database Updated to Release 10.1 : December 19, 2007 - Sanger miRBase microRNA Sequence Database Updated to Release 10.1. The miRBase sequence database1 is a comprehensive database of miRNA sequence data, annotation, and predicted gene targets and is the primary public repository for these data miRBase also provides a gene-naming service for assigning official miRNA names to novel miRNAs before they are published. miRBase provides a convenient, searchable online database, allowing the user to search by key word or sequence for information about known miRNAs and their targets and is freely available to all at http://microrna.sanger.ac.uk/.
LC Sciences - Careers : LC Sciences current position openings
LC Sciences - microRNA Detection Microarray Service - Product Detail : At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection. Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis. We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis. This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.
LC Sciences - microRNA, Discovery, Detection & Profiling Services - Product News : September 15th, 2009 - miRBase Sequence Database Updated to Release 14.0 March 9, 2009 - Sanger miRBase Sequence Database Updated to Release 13.0 Sept 23, 2008 - Number of Customer Publications Reaches 50. Sept 23, 2008 - Number of Customer Publications Reaches 50. - Sept 1, 2008 - Sanger miRBase Sequence Database Updated to Release 12.0 - May 6, 2008 - LC Sciences and DNAVision form Strategic Marketing Alliance - April 16, 2008 - Sanger miRBase Sequence Database Updated to Release 11.0 - February 27, 2008 – LC Sciences tops 30th microRNA service customer publication
LC Sciences - microRNA Detection Microarray Service - Product Literature : Product Brochure - microRNA Microarray Service - Technical Bulletin - µParaflo® Microfluidics Synthesis Technology - Technical Bulletin - Dual Sample vs. Single Sample Experiments - Technology Workshop - MicroRNA Profiling Using a High Performance, Flexible µParaflo® Biochip Platform - Technical Bulletin - Flexible microRNA Microarray Probe Content
LC Sciences - microRNA Detection Microarray Service - Application Notes : Characterization of breast cancer using microRNA expression profiling and epigenomic and proteomic measurements in rat model - microRNA profiling enables study of epigenetic mechanisms - microRNA profiling of FFPE tissue samples - microRNA profiling from blood / plasma samples - Small Non-coding RNA Discovery - miRecords: a new integrated resource for microRNA–target interactions
LC Sciences - microRNA, Discovery, Detection & Profiling Services - Web Resources : The miRBase Sequence Database - a searchable database of published miRNA sequences and annotation. The data were previously provided by the miRNA registry. The miRBase Registry continues to provide gene hunters with unique names for novel miRNA genes prior to publication of results. The miRBase Targets database is a new resource of predicted miRNA targets in animals. The latest release is version 11.0.
LC Sciences - microRNA Detection Microarray Service - Available Arrays : Our microRNA arrays cover all species for which sequence data are available in the miRBase Sequence Database . Please call if you don't see your species of interest. We can synthesize microRNA arrays for all species that are listed in the Sanger miRBase Sequence Database. We have listed a subset of our most popular species below. Please call if you don't see your species of interest. - Current List of All Available Species Microarrays - Commonly ordered arrays are listed below - Sanger miRBase Sequence Database - Special Announcement - miRBase Version 14.0 - Now Available!
LC Sciences - microRNA Detection Microarray Service - Pricing : For the comprehensive services, you just need to send us your total RNA sample(s) and let us know which microarray probe content you're interested in. We'll perform all the necessary functions to complete the microarray experiment and deliver your fully analyzed data in about 3-4 weeks Sample submission instructions Sample submission form
http://www.lcsciences.com/documents/miRNA_sample_submission_form_D.xls :
LC Sciences - microRNA Detection Microarray Service - FAQS (Frequently Asked Questions) : LC Sciences - microRNA Detection Microarray Service - FAQS (Frequently Asked Questions) A list of the most commonly asked questions regarding our microRNA microarray service.
LC Sciences - microRNA Detection Microarray Service - International Shipping Instructions : LC Sciences provides the following information to facilitate the international shipment of total RNA samples. We serve many customers overseas and shipping into the United States has not been a problem.
LC Sciences - microRNA Detection Microarray Service - Example Data : The original and processed microarray scan images. An array layout file. A raw intensity data file in Excel. A fully processed data file in Excel. A list of up and down regulated transcripts that are called based on a statistical analysis. Additionally, for each batch of samples, the customer receives a Data Summary containing a catalog of data files, images of representative regions of corresponding arrays, and descriptions of specific features of the arrays.
LC Sciences - microRNA Detection Microarray Service - Performance Data : µParaflo® microfluidic array platform of LC Sciences has an excellent assay reproducibility. Assay reproducibility is measured by how small chip-to-chip variation is for the same sample assayed over multiple chips. For each miRNA the assay variation across multiple chips is calculated using variation coefficient (CV)
LC Sciences - microRNA Microarray Service - Application Note - microRNA profiling of FFPE Sample Types : Archiving histological tissue samples by formalin-fixing and embedding in paraffin blocks has been a routine practice for medical and research institutions for many years and large collections of these samples now exist throughout the world. Estimates of sample numbers range from 300-400 million in the US alone covering virtually all known diseases. This number may be growing by tens of millions of samples per year.
LC Sciences - microRNA Microarray Service - Application Note - microRNA Profiling from Blood Samples : microRNA profiling from Blood / Plasma Samples. The regulatory effects microRNA of are widespread and microRNAs have recently been implicated in various diseases of the blood, stem cells, brain, and other tissues. MicroRNAs are expressed in the blood. A study involving sickle cell diseases revealed that there is significant microRNA expression in red blood cells and another showed that microRNAs are highly expressed in the ischemic brain and can be detected in blood samples.
LC Sciences - microRNA, Discovery, Detection & Profiling Services - Technical Discussion Group : This Yahoo hosted group provides an open forum for discussing topics related specifically to microRNA. Members post questions by sending an e-mail message to a list address. The message is then broadcasted to every member on the list. It's a great way to share ideas and information on this exciting new research topic. Our hope is that this group becomes the premier forum for microRNA discussions.
LC Sciences - microRNA, Discovery, Detection & Profiling Services : LC Sciences now offers a complete suite of analytical services to advance your microRNA research. These comprehensive services are designed to be one-stop and get you the results you need quickly to keep your research moving along. Our services now includes microRNA Sequencing for discovery and digital expression profiling applications. Next, our microRNA detection microarrays can provide differential expression profiles and are customizable to perform validation/confirmation of newly discovered microRNAs. Last, qRT-PCR is the gold standard for quantitation on microarray validation of microRNA expression.
LC Sciences - microRNA, Discovery, Detection & Profiling Services - Current News & Publications : LC Sciences now offers a complete suite of analytical services to advance your microRNA research. Here is the latest microRNA news and publications
LC Sciences - Peptide Microarray Service - PepArray Technology - Publications : Custom high density peptide synthesis and multiplex proteomics assays on a microchip based on the µParaflo® microfluidics technology. PepArray technology involves in situ (on chip) high density peptide synthesis and multiplex protein assays carried out in a microfluidic picoliter scale microarray. The PepArrayÔ is an assay reaction device which allows thousands of titration reactions, multiple step enzymatic reactions, and other biochemical reactions normally carried out in individual micro tubes or microtiter plates.
LC Sciences - Peptide Microarray Service - PepArray Technology - Technical Bulletins : Custom high density peptide synthesis and multiplex proteomics assays on a microchip based on the µParaflo® microfluidics technology. PepArray technology involves in situ (on chip) high density peptide synthesis and multiplex protein assays carried out in a microfluidic picoliter scale microarray. The PepArrayÔ is an assay reaction device which allows thousands of titration reactions, multiple step enzymatic reactions, and other biochemical reactions normally carried out in individual micro tubes or microtiter plates.
LC Sciences - microRNA Microarray Service - Application Note - microRNA Role in Immune System : MicroRNAs play a critical role in immune system by regulating/fine tuning inflammatory response MicroRNAs (miRNAs) have proven to be an extremely important part of the gene expression regulation mechanism. Expression profiling and functional studies indicate that miRNAs participate in the regulation of almost every cellular process investigated and that changes in their expression have a profound effect on their gene targeting activities. While the role of miRNAs in cell development and tumorigenesis was recognized early on, the importance of these small RNAs on immune system development and function has only been touched on recently.
LC Sciences - Targeted Sequencing - Sample Enrichment Service - Ordering : Please contact us for placing your order. - orders@lcsciences.com - (713) 664-7087 - 1-888-528-8818 - Fax(713) 664-81812575 West Bellfort Street, Suite 270, Houston, TX 77054 USA - www.lcsciences.com
LC Sciences - Targeted Sequencing - Sample Enrichment Service - Product Literature : Product Flyer - Targeted Sequencing - Sample Enrichment Service
LC Sciences - Targeted Sequencing - Sample Enrichment Service - FAQs : Frequently Asked Questions - General - Sample Preparation - Sample Submission - Deliverables
LC Sciences - Targeted Sequencing - Sample Enrichment Service - Service Description : LC Sciences’ µParaflo® technology is particularly well suited to provide sequence capture solutions through massively parallel synthesis of high quality DNA on biochips. Thousands of customer specified oligonucleotide sequences are in situ synthesized on a programmable high density microfluidics chip. Standard oligo synthesis chemistry and advance microfluidics design ensure high quality synthesis. Genomics samples can be applied directly to the biochip via microfluidics for on-chip sequence capture. Enriched targeted regions are recovered ready for sequencing.
LC Sciences - microRNA Sequencing Service - Ordering : LC Sciences now provides a one-stop solution for all your microRNA (miRNA) discovery and expression profiling needs. Total RNA Sample Preparation - Sample Amount Required - RNA Extraction - Quality Control of RNA Sample - Placing Your Order - Complete a Sample Submission Form - Packaging and Shipping
LC Sciences - microRNA Sequencing Service - Application Notes : microRNA Sequencing Service Application Notes - MicroRNA Discovery and Validation
LC Sciences - microRNA Sequencing Service - Pricing : Comprehensive Service - Total RNA to Data Service - Sample QC - Sample preparation (small RNA selection, library preparation, single molecule clonal amplification) - High-throughput sequencing (sequencing by synthesis) - Bioinformatics analysis (standard Solexa pipeline analysis) - Includes LCS software processing - data reduced to mappable reads - Customer data report - includes a list of sequences and counts
http://www.lcsciences.com/documents/microrna_sequencing_sample_submission.xls :
LC Sciences - Small RNA Discovery Sequencing Service - Ordering : LC Sciences provides a small RNA discovery service using the Solexa/Illumina high-throughput sequencing technology which enables comprehensive coverage, highly sensitive and specific discovery and profiling of all forms of small RNAs in your sample. Optional target-specific selection service is available to enrich your sample(s) for more focused sequencing to achieve deeper coverage. Down-stream profiling of identified (and other customer defined target small RNAs) is also available by custom microarray analysis service.
LC Sciences - Small RNA Discovery Sequencing Service - Pricing : Comprehensive Service - Total RNA to Data Service SRS-1001 -Additional Data Analysis Services - In Depth Data Analysis Service SRS-1007 - Map to Reference Sequence SRS-1008
http://www.lcsciences.com/documents/small_rna_sequencing_sample_submission.xls :
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Proteomics Microarrays : Our proteomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. Our comprehensive services includes sequence retrieval from public databases and literature, and/or assistance with your sequence designs, enzymatic and binding assays using samples provided by you, single or dual color detection using a suitable method, image data processing, and signal data processing.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Proteomics Microarrays - Ordering Instructions : Our specialty proteomics microarrays are custom products that can be used as part of a comprehensive project for biomolecule analysis. As these projects are often complex in nature, we ask that you please download and complete a project description form so that we may better understand your objectives for the experiment(s). A customer service representative will contact you within 24 hours to discuss the specifics of your project and give you an idea of the cost.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Proteomics Microarrays - Customer Publications : Our proteomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. In addition, the flexibility of µParaflo® enables the incorporation of modifications within the probes, which significantly expands the range of applications for these microarrays. Listed below are publications illustrating the application of custom microarrays containing non-standard probes. - Protein-Oligonucleotide Binding (Transcription Factor) Microarrays - Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain.
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LC Sciences - Company News & Events 2008 : March 10-11, 2008 - Cambridge, MA - microRNA in Human Disease and Development - We invite you to visit us at Booth #5 and attend our presentation: microRNA Profiling Using a High Performance, Flexible µParaflo® Biochip Platform Abstract: An advanced microfluidic biochip system designed to produce high quality data, stay current with the rapidly evolving microRNA field, and perform diverse small RNA discovery experiments is presented. This technology's unique flexibility allows for miRBase synchronicity and design of customized biochips adapted to each researcher's specific needs. Applications featuring disease marker discovery, drug treatment, microRNA target screening, and small RNA discovery are highlighted.
LC Sciences - Company News & Events 2007 : March 29-30, 2007 - Boston, MA - microRNA in Human Disease and Development April 15 - 18, 2007 - San Diego, CA - AACR 2007 - American Association for Cancer Research - Annual Meeting Sept 25, 2007 – Galveston, TX - UTMB Scientific Vendor Show Oct 23-27, 2007 - San Diego, CA - ASHG 2007 - American Society of Human Genetics - Annual Meeting Nov 4-7, 2007 - San Diego, CA - SFN 2007 - Society for Neuroscience - Annual Meeting Dec 2-4, 2007 - Washington, DC - ASCB 2007 - American Society for Cell Biology - Annual Meeting
LC Sciences - Company News & Events 2006 : Nov 1-3, 2006 – Cambridge, UK - MicroRNAs Europe April 2 - 5, 2006 – Washington, DC - AACR 2006 - American Association for Cancer Research - Annual Meeting Oct 10-12, 2006 – New Orleans, LA - ASHG 2006 - American Society of Human Genetics - Annual Meeting Oct 15-18, 2006 – Atlanta, GA - SFN 2006 - Society for Neuroscience - Annual Meeting
LC Sciences - microRNA Microarray Service - Technology Workshop : microRNAÂ PROFILING USING A HIGH PERFORMANCE, FLEXIBLE Î¼PARAFLOTMÂ BIOCHIP PLATFORM Agenda Î¼ParafloTMÂ Microfluidics Technology Applications Practical Considerations Q & A Microfluidic Array Platform Î¼ParafloTMÂ Microfluidics Chip 10 Âµl total volume thousands of reaction chambers Î¼ParafloTMÂ Microfluidics Chip in situ Synthesis Scheme Î¼ParafloTMÂ Microfluidics Chip A novel and proprietary solution photochemistry for producing high-fidelity oligo-DNA molecules
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http://www.lcsciences.com/documents/ncRNA_sample_submission.xls :
LC Sciences - Support Information : LC Sciences Technical Support Information - FAQs - Technical Bulletins / Application Notes - Publications - Technical Discussion Group - Web Resources - Email - support@lcsciences.com
LC Sciences - Support Information - FAQS : microRNA Detection Microarray Services - FAQs - OligoMix® - FAQs - Small Non-Coding RNA Discovery - Microarray Service - FAQs - Specialty Genomics Microarrays - FAQs - Kinase Profiling Microarray Service - FAQs - Protein Binding Microarray Service - FAQs
LC Sciences - OligoMix® - Oligonucleotide Mixture - Frequently Asked Questions : OligoMix® is a product that contains thousands of customer-specified oligonucleotide (oligo) sequences synthesized simultaneously in parallel, cleaved into solution, and delivered as a mix in a single microtube ready for many multiplex applications (Zhou et al., 2004; Tian et al., 2004). OligoMix® synthesis is based on a patented synthesis technology developed by our partner, Atactic Technologies (reviewed in Gao et al., 2004. This technology allows parallel synthesis of a large number of different oligo sequences using conventional DMT-chemistry (Caruthers 1991) at high yield. The synthesized sequences are removed from the surface and processed using our proprietary protocols to provide the final product.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Pricing : To prepare OligoMix®, we synthesize thousands of oligonucleotides at once in parallel and then cleave the oligos releasing them into solution for delivery. The base price for synthesis of each microchip is $735. We can synthesize up to 3,918 sequences per microchip. Also, the customer may request that we split the order into any number of multiple microchips. The basic length of OligoMix® sequences is 25 bases. If any of the sequences (it doesn’t matter how many) are over 25 bases, we add $5.25(N-25) to the base price where N is the length of the longest sequence.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Product Literature : Product Brochure - OligoMix® for Genomics Discoveries - Technical Bulletin - µParaflo® Microfluidics Synthesis Technology - Technical Bulletin - Sequence Capture for Targeted Sequencing
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes : Sequence Capture for Synthesis - Construction of Protein Coding Libraries - Gene Synthesis - Targeted Next-Gen Sequencing - Microarray QC - Sequence Specific Microarray Staining for Spotting Quality Control and Probe Validation - Limitations of Conventional Codon Randomization with Oligonucleotide Synthesis - siDNA, shDNA, or siRNA Vector Libraries - Small or Genome Scale Applications
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes - Protein Coding Libraries : OligoMix® can be used for complete de novo engineering of codon-specific protein-coding DNA libraries, i.e. Directed Evolution. Specific sequences of any codon choices, deletion, insertion, and length variations are easily made. The limitations of random codon generation are eliminated without expensive cost. OligoMix® enables fast turnaround in functional optimization of the designs of proteins.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes - Random Codon Limitations : Limitations of Conventional Codon Randomization with Oligonucleotide Synthesis - No flexibility in choosing codons for protein expression optimization. The NNN library has six fold difference in amino acid representation; the amount of the expressed full-length sequences drops quickly due to stop codon termination and codon-dependent low expression, and thus NNN is not suitable for mutations of more than ten residues. The NNC/T library is incomplete in representing amino acids (missing five of the 20 amino acids). The NNG/T and NNC/G/T libraries overcomes some limitations but still suffer from the difficulties of being inflexible in codon choices and sequence bias. The codon-based trinucleotide synthesis for building oligonucleotides provides specific codon for each amino acid, but it is expensive to be of routine use. None of the above method would allow deletion or insertions.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes - Gene Synthesis : Gene assembly strategies (overlapping, blunt-end, or staggered oligos) have been known for more than two decades. Existing barriers - cost and time required for obtaining oligonucleotides; for instance, for a small genome of 5 Mbp, 250,000 oligonucleotides of 40-mers are required. The synthesis per se would need 60,000 liters of solvents and cost multi-million dollars. PicoArray synthesis and OligoMix® provide an immediate answer to the problem since it can make tens of thousands of oligos at the cost and the time equivalent to those for making a few conventionally synthesized oligos.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes - Targeted Sequencing : Targeted Next-Generation Sequencing - Sequence Capture of Specific Gene Sets - Enrich genomic samples for targeted next-gen sequencing (Pathway specific gene sets, Cancer specific gene sets, etc.). Sequence Capture for Gene Expression - Capture selected transcript sets for deep sequencing (ex. miRNA expression). Multiplex Amplification - Generate custom primer sets to cover missing areas of unknown regions or for variation analysis.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes - Microarray QC : Microarray QC - Sequence Specific Microarray Staining for Spotting Quality Control and Probe Validation Obtain OligoMix containing all complementary strands of your probe sequences (your choices of fluorescent dye labeling) Hybridize these oligonucleotides to your microarray to quality test each probe. Detect problems with to improve the reliability of your microarrays. Use as a control reference set on all your microarray experiments to standardize results. Facilitate the design of high quality probes for diagnostic and biomarker detection applications.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes - siRNA Vector Libraries : OligoMix can be used to efficiently and cost effectively prepare siDNA, shDNA, or siRNA Vector Libraries - Small or Genome Scale Applications
LC Sciences - OligoMix® - Oligonucleotide Mixture - Product Literature - µParaflo® Microfluidics Synthesis Technology : The scientists and engineers at our technology partner, Atactic Technologies, have developed platform technologies that encompass a new class of microfluidic µParaflo® reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns.
LC Sciences - OligoMix® - Oligonucleotide Mixture - Product Literature - Sequence Capture for Targeted Sequencing : Recent major advances in DNA sequencing technologies have resulted in several new “next-generation” platforms capable of generating massive amounts of reads very quickly and relatively inexpensively. These new technologies hold the promise of one day, routinely sequencing entire complex eukaryotic genomes. Currently, these new technologies do not have quite the through-put required yet for routine whole genome sequencing. At this point, they are more effectively utilized for systematic study of genetic variation in certain targeted regions (subsets) of complex genomes. By targeting specific regions, one can take better advantage of next-gen sequencing capability; essentially, more coverage is achieved by focusing reads on your area of interest.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Application Notes - Sequence Capture : Sequence Capture of Specific Gene Sets - Enrich genomic samples for targeted next-gen sequencing (Pathway specific gene sets, Cancer specific gene sets, etc.). Sequence Capture for Gene Expression - Capture selected transcript sets for deep sequencing (ex. miRNA expression).
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Product Literature : LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Product Literature - Technical Bulletin - Sequence Capture for Targeted Sequencing
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Application Notes : Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. In addition, the flexibility of µParaflo® enables the incorporation of modifications within the probes, which significantly expands the range of applications for these microarrays. Listed below are applications of custom microarrays containing non-standard probes along with selected reference
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Publications : Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. In addition, the flexibility of µParaflo® enables the incorporation of modifications within the probes, which significantly expands the range of applications for these microarrays. Listed below are publications illustrating the application of custom microarrays containing non-standard probes.
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - FAQs : The most frequenctly asked questions regarding our specialty genomics microarrays...
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Pricing : Base Pricing - Longer Sequences Pricing - Modifications Pricing - Services Pricing
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Genomics Microarrays - Technical Bulletin - Sequence Capture : Recent major advances in DNA sequencing technologies have resulted in several new “next-generation” platforms capable of generating massive amounts of reads very quickly and relatively inexpensively. These new technologies hold the promise of one day, routinely sequencing entire complex eukaryotic genomes. Currently, these new technologies do not have quite the through-put required yet for routine whole genome sequencing. At this point, they are more effectively utilized for systematic study of genetic variation in certain targeted regions (subsets) of complex genomes. By targeting specific regions, one can take better advantage of next-gen sequencing capability; essentially, more coverage is achieved by focusing reads on your area of interest.
LC Sciences - Kinase Profiling Microarray Service - Frequently Asked Questions : FAQs - General - What are PKS - peptide microarrays? Microarray Design - Custom Sequences - Sample Preparation - Sample Submission - Array Results - Data Analysis - Additional Questions
LC Sciences - Kinase Profiling Microarray Service - Available Arrays : Standard Array - KPA-1001 A predesigned microarray containing known protein kinase substrates built on a µParaflo® microfluidics peptide chip. Our standard content protein kinase substrate (PKS) - peptide microarray includes a comprehensive list of kinases and their related experimentally verified Serine, Threonine, and Tyrosine phosphorylation sites. - Custom Array - KPA-2001 - A custom microarray containing peptide sequences determined by the customer built on a µParaflo® microfluidics peptide chip. We can synthesize a completely custom microarray designed specifically for your experiment. Our basic chip design has 4K features. Basic peptide length is 8 residues. (Additional length is possible.) You can use available web tools to assist with your design. (See Example) Or contact us for assistance with design of your PKS- Peptide Microarray.
LC Sciences - Kinase Profiling Microarray Service - Pricing : Standard Array Content Option KPA-1001 - Custom Array Content Option KPA-2001 - Additional Peptide Synthesis (length beyond 8mer)KPA-3001 - Comprehensive Services Pricing - Comprehensive Kinase Microarray Service KAS-1001 - Additional Services Pricing - Custom Peptide Microarray Design Assistance AS-1009 - In-Depth Data Analysis KAS-1007 - Quotes- If you are unsure about your particular service needs, please make use of our Peptide Microarray Service - Project Description Form.
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LC Sciences - Kinase Profiling Microarray Service - Design Example : First we compiled a list of known substrate peptides. (Shown in Table 1) Next we used WebLogo to create sequences logos for each of these protein kinases. The height of the letter is proportional to the probability of a specific amino acid at that particular position. Important - Note that there is no information on sequence preference in the WebLogo display since the probability of an amino acid at a position is independent of the adjacent positions. Figure 1 is the sequence logo for the protein kinase EGFR. Next we listed the six most probable amino acids on each side of the phosphorylation site (Table 2). Peptide sequence is numbered from N- (minus number) to C- (positive #) terminal and the phosphorylation site is 0.
LC Sciences - Kinase Profiling Microarray Service - Application Notes - Quantitative Measurement of Kinase Kinetic Activities Including PKA Studies : Kinetic PKA kinase studies on a pico-liter titrator microarray - Chip Design - PKS - peptide microarray - Density variation synthesis creates a pico-liter titrator microarray. S subgroup - peptides Ac-RRXSLG (PKA substrate consensus) and Ac-KRXSLG (PKC substrate consensus) with 10 different densities from x32 density dilution to 16x density increasing. A group - peptides Ac-RRXALG and Ac-KRXALG with different densities as negative controls for the S subgroup.. pS positive control - a synthetic phosphopeptide Ac-RRQpSLG. T group - an Ac-RRXTLG subgroup. Others - peptide substrates at Ser/Thr kinase: GSK-3, PKB, etc. - Assay - Sample - cAMP-dependent Protein Kinase (PKA) catalytic subunit (New England Biolabs). Enzyme concentration - 2,500 units. Volume - 50 µl. Reaction time - 30 minutes or longer. - Detection - Fluorescent dye is used to specifically stain phosphate groups (pS, pT, and pY). Only the phosphate group of the phosphopeptides will be specifically stained by the fluorescent dye. No need for antibodies which are known to suffer from non-specific or weak binding problems. If specially request, antibody detection can be applied.
LC Sciences - Kinase Profiling Microarray Service - Scientific Publications : High density peptide microarrays. In situ synthesis and applications. In situ parallel synthesis of addressable peptide microarrays. Light directed massively parallel on-chip synthesis of peptide arrays with t-Boc chemistry. Individually addressable parallel peptide synthesis on microchips. Peptide synthesis based on t-Boc chemistry and solution photogenerated acids.
LC Sciences - Kinase Profiling Microarray Service - Product Literature : Product Flyer - Kinase Profiling Microarray Service - Product Flyer - Biomarker Screening, Discovery & Detection Microarray Service - Technical Bulletin - µParaflo® Microfluidics Synthesis Technology - Technical Bulletin - Massively parallel peptide synthesis on a microchip based on the µParaflo® microfluidics technology
LC Sciences - Kinase Profiling Microarray Service - Application Notes : Large Scale Mapping of Kinase Substrate Specificity - Quantitative Measurement of Kinase Kinetic Activities Including PKA Studies - Other Applications
LC Sciences - Kinase Profiling Microarray Service - Application Notes - Large Scale Mapping of Kinase Substrate Specificity : Quantitative mapping of substrate specificity for protein kinase Src on a microarray - Chip Design - PKS - peptide microarray. 27 known protein tyrosine kinase substrates and their sequence variants. All peptides on the chip contain 8-10 redundancies.. Positive controls - synthetic phosphopeptides (pY phosphotyrosine incorporated by synthesis). Negative controls - Ala substitution of Tyr at the phosphorylation site. Src kinase, p60c-src (Invitrogen) - Assay - Sample - Src kinase, p60c-src (Invitrogen). Enzyme concentration - 0.5 mg/ml. Volume - 50 µl. Reaction time - 30 minutes or longer - Detection - Fluorescent dye is used to specifically stain phosphate groups (pS, pT, and pY). Only the phosphate group of the phosphopeptides will be specifically stained by the fluorescent dye. No need for antibodies which are known to suffer from non-specific or weak binding problems. If specially request, antibody detection can be applied.
LC Sciences - Kinase Profiling Microarray Service - Application Notes - App 1 Fig 1 :
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LC Sciences - Kinase Profiling Microarray Service - Applications : Protein Kinase Profiling on a Proteomic Scale - Large scale mapping of kinase substrate specificity - Detecting and discovering novel kinases - Mapping phosphorylation sites - Validating computationally predicted or other types of kinase substrate peptide candidates - Quantitative Protein Kinase Kinetics Studies - Making quantitative measurement of protein kinase kinetic activities - Performing PKA studies - Compare protein kinases and identify the changes - Drug Development - Screening inhibition of kinase enzymatic activities by competition assays - Identifying substrate peptides as biomarkers - Detecting cell signaling activities - Studying signaling pathways using multiplex reactions - Monitoring apoptosis, cancer and cell development - Providing parameters for systems modeling - Post Proteomic Tools
LC Sciences - Kinase Profiling Microarray Service - Technical Bulletins - µParaflo® Microfluidics Synthesis Technology : The scientists and engineers at our technology partner, Atactic Technologies, have developed platform technologies that encompass a new class of microfluidic µParaflo® reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers at high yield without the need for the expensive, inconvenient microfabricated photomasks previously used.
LC Sciences - Kinase Profiling Microarray Service - Application Notes - App 2 Fig 1 :
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LC Sciences - Peptide Microarray Service - Sample Submission : Protein Sample Submission Instructions - Sample Type - Sample State - Sample Purity - Sample Amount - Binding Assays - Enzymatic Assays - Packaging and Shipping
LC Sciences - Protein Binding Microarray Service - Frequently Asked Questions : Frequently Asked Questions - General - What are protein binding - peptide microarrays? - Microarray Design - Sample Preparation - Sample Submission - Array Results - Data Analysis - Additional Questions
LC Sciences - Protein Binding Microarray Service - Pricing : Custom Peptide Microarray PA-2001 - Additional Peptide Synthesis (length beyond 8mer) PA-3001 - Comprehensive Services Pricing - Protein Binding Microarray - Comprehensive Service (Direct Detection Option) PAS-1001 - Protein Binding Microarray - Comprehensive Service (Specific Antibody Detection Option) PAS-1002 - Protein Binding Microarray - Comprehensive Service (Secondary Antibody Detection Option) PAS-1003 - Additional Services Pricing - Custom Peptide Microarray Design Assistance AS-1009 - In-Depth Data Analysis AS-1007
LC Sciences - Protein Binding Microarray Service - Web Resources : PepCyber :P~Pep - a database of human protein-protein interactions mediated by phosphoprotein binding domains (PPBDs). - WebLogo - A web based application designed to make the generation of sequence logos as easy and painless as possible. Sequence logos are a graphical representation of an amino acid or nucleic acid multiple sequence alignment developed by Tom Schneider and Mike Stephens. Each logo consists of stacks of symbols, one stack for each position in the sequence. The overall height of the stack indicates the sequence conservation at that position, while the height of symbols within the stack indicates the relative frequency of each amino or nucleic acid at that position. In general, a sequence logo provides a richer and more precise description of, for example, a binding site, than would a consensus sequence.
LC Sciences - Protein Binding Microarray Service - Scientific Publications : High density peptide microarrays. In situ synthesis and applications. In situ parallel synthesis of addressable peptide microarrays. Light directed massively parallel on-chip synthesis of peptide arrays with t-Boc chemistry. Individually addressable parallel peptide synthesis on microchips. Peptide synthesis based on t-Boc chemistry and solution photogenerated acids.
LC Sciences - Protein Binding Microarray Service - Product Literature : Product Flyer - Epitope Mapping Microarray Service - Product Flyer - Biomarker Screening, Discovery & Detection Microarray Service - Technical Bulletin - µParaflo® Microfluidics Synthesis Technology - Technical Bulletin - Massively parallel peptide synthesis on a microchip based on the µParaflo® microfluidics technology
LC Sciences - Protein Binding Microarray Service - Application Notes : Kinase Profiling Applications - Epitope Mapping Applications - Phosphopeptide Binding Applications - Aptamer Binding Applications - Picoliter Scale Titer Plate Applications
LC Sciences - Protein Binding Microarray Service - Application Notes - Quantitative Measurement of AFM2 Epitope Peptide Binding : Quantitative Measurement of AFM2 Epitope Peptide Binding - Chip Design - Epitope (known) DYKDDDDK - 312 Epitope and variant sequences - >4 redundancy - CV < 15% - Assay - Antibody: anti-flag peptide (AFM2) (1 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy3 - Results - Figure 1 - Fluorescent image of 4K peptide chip from AFM2 binding
LC Sciences - Protein Binding Microarray Service - Application Notes - App 1 Fig 1 :
LC Sciences - Protein Binding Microarray Service - Application Notes - App 1 Fig 2 :
LC Sciences - Protein Binding Microarray Service - Application Notes - Quantitative Measurement of Anti HA Epitope Peptide Binding : Quantitative Measurement of Anti HA Epitope Peptide Binding - Chip Design - Epitope (known) YPYDVPDYA - Chip (1) 800 HA and flag epitope and variant sequences - >3 redundancy - CV < 15% - Chip (2) HA, c-myc, VSVG, and flag epitope and other peptide sequences - >3 redundancy - CV < 30% - Assay - Antibody: anti-HA (100-1,000 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy5-IgG - Results - Figure 1 - Fluorescent image of Anti HA binding to epitope and its variants
LC Sciences - Protein Binding Microarray Service - Application Notes - App 2 Fig 1 :
LC Sciences - Protein Binding Microarray Service - Application Notes - App 2 Fig 2 :
LC Sciences - Protein Binding Microarray Service - Application Notes - Anti HA Binding: Association curves measured with protein concentration titration on Epitope Peptide chip : Anti HA Binding: Association curves measured with protein concentration titration on Epitope Peptide chip - Chip Design - Epitope (known) YPYDVPDYA - N-truncation - C-truncation - DYK plus N-truncation - DYK plus C-truncation - Assay - Antibody: anti-HA (0.1-60,000 ng/ml) - Detection - Signal intensities acquired at different scanning gains were scaled according to calibration curve. Curve fitting used Origin (Origin Lab). - Results - Figure 1 -Plots of association curves.
LC Sciences - Protein Binding Microarray Service - Application Notes - App 3 Fig 1 :
LC Sciences - Protein Binding Microarray Service - Application Notes - Anti HA Binding: Association curves measured with peptide concentration (density) variation by in situ synthesis of Epitope Peptide Chip : Anti HA Binding: Association curves measured with peptide concentration (density) variation by in situ synthesis of Epitope Peptide Chip. - On chip measurements of anti HA binding to epitope YPYDVPDYA. - Illustration of a method for high throughput measurements of protein-peptide interactions. - Chip Design - Epitope (known) YPYDVPDYA - N-truncation - C-truncation - DYK plus N-truncation - DYK plus C-truncation - Assay - Antibody: anti-HA (100 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Detection through secondary antibody with dye(cy3 or cy5) conjugation (100 mg/ml) - Results - Figure 1 - Image of the chip and plot of the chip data.
LC Sciences - Protein Binding Microarray Service - Application Notes - App 4 Fig 1 :
LC Sciences - Protein Binding Microarray Service - Application Notes - App 4 Fig 2 :
LC Sciences - Protein Binding Microarray Service - Application Notes - Quantitative Measurement of Anti c-myc Epitope Peptide Binding : Quantitative Measurement of Anti c-myc Epitope Peptide Binding - Chip Design - Epitope (known) EQKLISEEDL - HA, c-myc, VSVG, and flag epitope and other peptide sequences (see figure 3 below for detail) - >3 redundancy - CV < 30% - Assay - Antibody: anti-c-myc (1 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy5-IgG (100ng/ml) - Results - Figure 1 - Fluorescent images of 4K peptide chip for 3 different experiments
LC Sciences - Protein Binding Microarray Service - Application Notes - App 5 Fig 1 :
LC Sciences - Protein Binding Microarray Service - Application Notes - App 5 Fig 2 :
LC Sciences - Protein Binding Microarray Service - Application Notes - Quantitative Measurement of Anti VSVG Epitope Peptide Binding : Quantitative Measurement of Anti VSVG Epitope Peptide Binding - Chip Design - Epitope (known) YTDIEMNRLGK - HA, c-myc, VSVG, and flag epitope and other peptide sequences (see figure 3 below for detail) - >3 redundancy - CV < 30% - Assay - Antibody: anti-VSVG(1 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy5-IgG (100ng/ml) - Results - Figure 1 - Fluorescent image and detail of chip layout
LC Sciences - Protein Binding Microarray Service - Application Notes - App 6 Fig 1 :
LC Sciences - Protein Binding Microarray Service - Application Notes - App 6 Fig 2 :
LC Sciences - Protein Binding Microarray Service - Applications : Immunological Studies - Identifying specific substrate for a known antibody - Identifying immunodominant regions in antigens - Quantitative measurement of binding curves - Multiplex antigen binding assays - Developing immunological tools for clinical applications - Studying immune networks - Vaccine Development - Developing specific antibodies by pre-screening cross-reactivity - Mapping antibody cross-reactivity - Developing new antigen agents for novel antibodies - Developing epitope-vaccine therapies - Developing inhibitor of antigen-antibody - Biosensor Development - Detecting autoimmune-response antibodies - Screening biomarkers from biological samples such as blood serum or cell lysate
LC Sciences - Protein Binding Microarray Service - Technical Bulletins - µParaflo® Microfluidics Synthesis Technology : µParaflo® Microfluidics Synthesis Technology - The scientists and engineers at our technology partner, Atactic Technologies, have developed platform technologies that encompass a new class of microfluidic µParaflo® reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers at high yield without the need for the expensive, inconvenient microfabricated photomasks previously used.
LC Sciences - Technical Bulletins : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Support - Technical Bulletins / Application Notes
LC Sciences - Protein - Aptamer Binding Microarray Service - Application Notes - Mapping a specific binding sequence of an aptamer oligonucleotide : Mapping a specific binding sequence of an aptamer oligonucleotide to decrease the molecule size for many practical applications such as drug molecules - Identification of key positions of a consensus sequence for enhancing binding affinity (possibly by modifying those positions) for an aptamer drug candidate. Through this strategy, we can map a small but highly specific binding aptamer oligonucleotide. Background - Macugen (pegaptanib sodium) is an ophthalmic aptamer drug being developed by Pfizer/Eyetech for the treatment of the 'wet' forms of age-related macular degeneration (AMD). The consensus sequence is CGGAAUCAGTGAATGCTTATACATCCGt, an RNA 28 mer where all pyrimidines are modified with a 2'-F and the all the purines (except 2 As) are modified with a 2'-'OMe group. Our aptamer chip is a powerful tool for further refining the consensus sequences identified from the in vitro evolution (SELEX), by finding the minimal length of oligonucleotide required for specific binding (core sequence) and systematic modification of the core sequence. The above example shows a strategy for searching the region of core sequence, which will show highest binding signals.
LC Sciences - Protein - Aptamer Binding Microarray Service - Available Arrays : Standard Array - A predesigned microarray built on a µParaflo® microfluidics chip. Our standard aptamer microarray includes >1000 known DNA aptamers. DNA, 2'-OMe RNA, and 2'-F RNA synthesis chemistries are available. Complete list of standard content - Custom Array - A custom microarray containing aptamer sequences determined by the customer built on a µParaflo® microfluidics chip. We can synthesize a completely custom microarray designed specifically for your experiment. Our basic chip design has 4K features. DNA, 2'-OMe RNA, and 2'-F RNA synthesis chemistries are available. Contact us for assistance with design of your DNA/RNA aptamer microarray.
LC Sciences - Protein - Aptamer Binding Microarray Service - Pricing : Standard Array Content Option - All standard aptamer microarrays are synthesized on a µParaflo® microfluidics chip >1000 standard aptamer sequences (see probe content) - Aptamer Microarray - Standard (DNA)AA-1001 - Aptamer Microarray - Standard (2'-OMe RNA)AA-1002 - Custom Array Content Option - Synthesis of a custom designed DNA/RNA aptamer microarray on a µParaflo® microfluidics chip, length up to 25mer. Up to 4K customer defined sequences - Customizable features incvlude - sequence design, varying lengths, chip layout, synthesis chemistry, and more! - Aptamer Microarray - Custom (DNA)AA-2001 - Aptamer Microarray - Custom (2'-OMe RNA)AA-2002 - Additional DNA/RNA Synthesis (length beyond 25mer) - Synthesis of additional DNA bases DA-3001 - Synthesis of additional 2'-OMe bases RA-3002 - Additional cost only applies to bases beyond 25mer.
http://www.lcsciences.com/documents/aptamer_microarray_content.xls :
LC Sciences - Protein - Aptamer Binding Microarray Service - Scientific Publications : Application Articles (Aptamer Oligonucleotide Microarrays)
LC Sciences - Protein - Aptamer Binding Microarray Service - Application Notes : Discovery of Protein-Binding DNA Sequences on an Aptamer Oligonucleotide Microarray - Mapping a specific binding sequence of an aptamer oligonucleotide to decrease the molecule size for many practical applications such as drug molecules - Other Applications - Protein-Nucleic Acid Binding - Drug Screening - Target Validation - Biosensor Screening - Biomarker Screening - Diagnostic Chip Development - Inhibitor Assays -
LC Sciences - Protein - Aptamer Binding Microarray Service - Application Notes - Discovery of Protein-Binding DNA Sequences on an Aptamer Oligonucleotide Microarray : Discovery of Protein-Binding DNA Sequences on an Aptamer Oligonucleotide Microarray - Identify specific sequences. Differentiate single strand vs. duplex binding. Identify high definition patterns of the binding. Chip Design - Contains both single stranded and duplex oligonucleotides - Varying sequence lengths - Assay - Sample - CUGBP1 is a Myotonic Dystrophy (DM1) related protein - Detection - Anti-His and Cy3 IgG - Results - Figure 1 - Schematic of detection with secondary antibody on aptamer microarray
LC Sciences - Protein - Aptamer Binding Microarray Service - Application Notes - Fig 1 :
LC Sciences - Protein - Aptamer Binding Microarray Service - Application Notes - Fig 2 :
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LC Sciences - Specialty Proteomics Microarrays - Application Notes - SHP2 and SH2 Proteins Assayed on a Phosphopeptide Microarray : Chip Design - More than 940 sequences plus controls and reference sequences - Typical Variations: EPIXAKVNKKVN X=pY, Y, A and possibly other amino acids, length variant sequences. - >3 redundancy - Assay - 0.2 µM protein - TBST buffer - Room Temp - Reaction time: 2 hours - Detection - Cy5-IgG - Results - Figure 1 - 3 Fluorescent images (inverted) of 4K peptide microarray after protein binding
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Proteomics Microarrays - Product Literature : Product Flyer - Biomarker Screening, Discovery & Detection Microarray Service - Technical Bulletin - Massively parallel peptide synthesis on a microchip based on the µParaflo® microfluidics technology - Technical Bulletin - µParaflo® Microfluidics Synthesis Technology
LC Sciences - Technologies for Genomics and Proteomics Discoveries - Specialty Proteomics Microarrays - Application Notes : SHP2 and SH2 Proteins Assayed on a Phosphopeptide Microarray - Peptidomimetics Studies - Density Variation Synthesis Creates a Picotiter Peptide Microarray - Other Applications - Enzyme reactivity studies - Pathway studies - Protein-protein interactions - Phosphotyrosine binding protein (PTB) studies
LC Sciences - Specialty Proteomics Microarrays - Application Notes - Peptidomimetic Studies : Peptide chip containing peptidomimetics.
LC Sciences - Specialty Proteomics Microarray Service - Application Notes - App 2 Fig 1 :
LC Sciences - Specialty Proteomics Microarrays - Application Notes - Density Variation Synthesis Creates a Picotiter Peptide Microarray : Density Variation Synthesis Creates a Picotiter Peptide Microarray. Create peptides of different concentrations on chip.
LC Sciences - Specialty Proteomics Microarray Service - Application Notes - App 3 Fig 1 :
LC Sciences - Specialty Proteomics Microarray Service - Application Notes - App 1 Fig 1 :
LC Sciences - Specialty Proteomics Microarray Service - Application Notes - App 1 Fig 2 :
LC Sciences - Specialty Proteomics Microarray Service - Application Notes - App 1 Fig 3 :
LC Sciences - Specialty Proteomics Microarray Service - Application Notes - App 1 Fig 4 :
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LC Sciences - Support Information - Publications : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Customer Publications - microRNA Microarray Service - Specialty Proteomics Microarrays - Technology Publications - µParaflo® in situ Microarray Synthesis Technology
LC Sciences - Support Information - Web Resources : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. microRNA Detection Microarray Services - Web Resources - Protein Binding Microarray Service - Web Resources
LC Sciences - Gene Expression Analysis Service - Available Arrays : LC Sciences is a genomics and proteomics company offering innovative and quality products and services. Our Gene Expression Analysis Service is available for all species for which GeneChip®brand arrays exist. See the Affymetrix website for a comprehensive list of available GeneChip® brand arrays. Listed below are the Affymetrix GeneChip® brand arrays that match the species of our microRNA detection microarrays.
LC Sciences - Targeted Sequencing - Sample Enrichment Service - Pricing : Please contact us for a quote. - sales@lcsciences.com - (713) 664-7087 - 1-888-528-8818 - Fax(713) 664-81812575 West Bellfort Street, Suite 270, Houston, TX 77054 USA - www.lcsciences.com
LC Sciences - Targeted Sequencing - Sample Enrichment Service - International Shipping Instructions : LC Sciences provides the following information to facilitate the international shipment of samples from overseas. We serve many customers overseas and shipping into the United States has not been a problem.
http://www.lcsciences.com/documents/all_species_mirna_arrays.xls :
LC Sciences - OligoMix® - Oligonucleotide Mixture - Application Notes - Sequence Capture For Synthesis : A collaboration by researchers from Harvard Medical School, the University of Houston, and University of Michigan demonstrates in solution hybridization selection as an effective method to prepare synthetic building blocks for high fidelity gene synthesis. - Accurate multiplex gene synthesis from programmable DNA microchips - Gene synthesis is a powerful tool to test many hypotheses from genomics and systems biology experiments but it demands accurate synthetic building blocks for successful gene assembly. In this publication, the researchers describe a method for hybridization selection using a pool of thousands of selection oligonucleotides coupled to beads to selectively remove oligonucleotides with mutations prior to gene assembly an increase the fidelity of assembled gene nine-fold.
LC Sciences - microRNA qRT-PCR Service - Available Assays : Our microRNA qRT-PCR service makes use of TaqMan® MicroRNA Assays to quantitate microRNA expression. Every TaqMan® MicroRNA Assay has been functionally validated under laboratory conditions to ensure accurate results. Download a list of all TaqMan® MicroRNA Assays showing part numbers, target sequences, miRBase names, miRBase accession numbers, and target species.
LC Sciences - microRNA qRT-PCR Service - Pricing : Comprehensive Services - Total RNA to Data Service - Our service is comprehensive and includes small RNA enrichment from your total RNA sample, TaqMan® microRNA assay (in triplicate), and fully analyzed (t-test) quantitative data. Assay - analysis of 1 sample and 1 miR. Each assay includes 3 reactions (replicates) - Relative Quantitation - For multiple sample experiments (e.g. treated and untreated) customer should suggest one or preferable two endogenous controls. We will add an assay of each sample with the selected control probe(s) to the plate in order to calculate relative quantitation between samples. t-test will be performed. Price per control assay is the same, $30.
LC Sciences - microRNA qRT-PCR Service - Controls : Endogenous Controls - For each sample, we suggest using at least one, preferably two, small RNA controls from the following list.[1] If you already have small RNA controls of your own preference, please contact us about availability. Human tissues - RNU48 - U47 - RNU6B - Mouse tissues - snoRNA202 - snoRNA234 - Cell lines - RNU24 - RNU38B - Z30
LC Sciences - Small Non-Coding RNA Discovery Microarray Service - Detail : Non-coding (ncRNAs) are a class of RNAs that do not encode proteins but instead possess regulatory function at the level of RNA in the cell. Custom microarrays have been shown to be a effective experimental approach to discovery of novel ncRNAs as well as a method to experimentally test computationally predicted ncRNAs. LC Sciences provides a small non-coding RNA discovery service using innovative µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of small RNAs in your sample. This service is comprehensive; from array design to sample to data, allowing the most efficient novel discovery of small regulatory/functional RNA embedded within non-coding RNAs (ncRNAs) or genomic RNAs.
LC Sciences - Samll Non-Coding RNA Discovery Microarray Service - Customer Publications : Novel MicroRNA Candidates and miRNA-mRNA Pairs in Embryonic Stem (ES) Cells - Identification and characteristics of microRNAs from Bombyx mori - Identification of conserved microRNAs and their targets from Solanum lycopersicum Mill - A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline - The colorectal microRNAome
LC Sciences - Small Non-Coding RNA Discovery Microarray Service - Product Literature : Product Flyer - Small Non-coding RNA Discovery - Microarray Service
LC Sciences - Small Non-Coding RNA Discovery Microarray Service - Application Notes : Application Note - Small Non-coding RNA Discovery
LC Sciences - Small Non-Coding RNA Discovery Microarray Service - Pricing : Comprehensive Services - Total RNA to Data Service - Single Sample Array Option NCAS-1001 - Total RNA to Data Service - Dual Sample Array Option NCAS-2001 - Additional Data Analysis Services - In Depth Data Analysis Service AS-1007 - Correlation to Previous Results AS-1014 - Optional Individual Services - Bioanalyzer QC Service AS-1002 - Small Size - Sample Preparation Service AS-1012 - Rush Service AS-1008
LC Sciences - Small Non-Coding RNA Discovery Microarray Service - Frequently Asked Questions : Microarray Design - Custom Sequences - Sample Preparation - Sample Submission - Sample Preparation and Labeling - Array Results - Data Analysis - Additional Questions
LC Sciences - microRNA Detection Microarray Service - Dual Sample vs Single Sample : Dual Sample vs Single Sample Experiments - Dual Sample - A dual-sample assay is used when two samples are compared with each other. The assay does not involve chip-to-chip variations and therefore may reveal very small differences between the two samples. The assay is especially suitable when paired samples are studied, such as diseased tissue being compared with an adjacent non-diseased tissue. The assay also has an advantage of lower per-sample cost than single sample assay does. However, design considerations should be given to minimize dye-related bias. - Single Sample - A single-sample assay is used when multiple independent samples are compared with each other. The assay has the advantage of free of dye-related bias although it has a higher per-sample cost.
LC Sciences - microRNA Microarray Service - Product News - Sept 23, 2008 - Number of Customer Publications Reaches 50! : LC Sciences is proud to announce the recent publication of the 50th peer-reviewed study by one of its customers using the company’s microarray service for analyzing microRNA expression profiles and for discovery of novel small RNAs. These studies, by leading researchers in the field, contribute to a fast growing body of knowledge defining this recently discovered class of regulatory RNAs.
LC Sciences - microRNA Microarray Service - Product News - August 2, 2007 - Sanger miRBase microRNA Sequence Database Updated to Release 10.0 : August 2, 2007 - Sanger miRBase microRNA Sequence Database Updated to Release 10.0. The study of microRNA (miRNA) is a relatively new field with the first significant publications appearing in 2001. Our knowledge continues to grow rapidly as more and more researchers race to discover new miRNAs and uncover the importance of these small regulatory elements. Recent publications have linked miRNAs with a wide range of biological functions and as new miRNAs are discovered and experimentally verified, the data are deposited to the public database, miRBase.
LC Sciences - microRNA Microarray Service - Product News - July 12, 2007 - Mammalian microRNA Atlas Published : July 12, 2007 - Mammalian microRNA Atlas Published. In order to identify miRNAs and to assess their expression patterns, a consortium of researchers sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents1. As a result, many new mature Human, Mouse, and Rat microRNA sequences were submitted to the Sanger miRBase Sequence Database. Although not yet released as a new miRBase version, This probe content is immediately available on all of our standard or custom microRNA microarrays.
LC Sciences - microRNA Microarray Service - Product News - May 25, 2007 - Sanger miRBase microRNA Sequence Database Updated to Release 9.2 : May 25, 2007 - Sanger miRBase microRNA Sequence Database Updated to Release 9.2. The study of microRNA (miRNA) is growing rapidly as researchers discover new miRNAs and uncover the importance of these small regulatory elements linked to a wide range of biological functions. As new miRNAs are discovered and experimentally verified, the data are deposited to the public database, miRBase. The miRBase sequence database1 is a comprehensive database of miRNA sequence data, annotation, and predicted gene targets and is the primary public repository for these data. miRBase also provides a gene-naming service for assigning official miRNA names to novel miRNAs before they are published. http://microrna.sanger.ac.uk/.
LC Sciences - microRNA Microarray Service - Technical Bulletins - µParaflo® Microfluidics Synthesis Technology : µParaflo® Microfluidics Synthesis Technology. The scientists and engineers at our technology partner, Atactic Technologies, have developed platform technologies that encompass a new class of microfluidic µParaflo® reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes. The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns.. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers at high yield without the need for the expensive, inconvenient microfabricated photomasks previously used.
LC Sciences - microRNA Microarray Service - Application Note - Characterization of breast cancer using microRNA expression profiling : Characterization of breast cancer using microRNA expression profiling and epigenomic and proteomic measurements in rat model. This month researchers at the Department of Biological Sciences at the University of Lethbridge published the latest in a series of papers describing characterization of breast cancer using microRNA expression profiling and epigenomic and proteomic measurements in rat model.
LC Sciences - microRNA Microarray Service - Application Note - microRNA profiling enables study of epigenetic mechanisms : microRNA profiling enables study of epigenetic mechanisms. Micro RNAs (miRNAs) are small non-protein-coding RNA molecules that function as negative regulators of gene expression by base pairing with specific mRNAs. This either inhibits translation or promotes mRNA degradation. While their crucial function in regulation of genes involved in the control of development, cell proliferation, apoptosis, and stress response is well established, much less is known about their role in carcinogenesis. Significantly altered miRNA levels were observed in full-fledged tumors but specifically at the early pre-cancerous stage little data are available. Two recent publications by researchers from the Department of Biological Sciences at the University of Lethbridge give important new insight.
LC Sciences - microRNA Microarray Service - Application Note - Small Non-coding RNA Discovery : Non-coding (ncRNAs) are a class of RNAs that do not encode proteins but instead possess regulatory function at the level of RNA in the cell. There is much data that suggests that there are likely many more ncRNAs that exist, yet to be discovered. Custom microarrays have been shown to be an effective experimental approach to discovery of novel ncRNAs1 as well as a method to validate candidate ncRNAs2.
LC Sciences - microRNA Microarray Service - Application Note - miRecords: a new integrated resource for microRNA–target interactions : While much progress has been made in computational target prediction of miRNAs in recent years and more than 10 miRNA target prediction programs have been established, the prediction of miRNA targets remains a challenging task.The group of Dr. Tongbin Li from the Department of Neuroscience, University of Minnesota has developed miRecords, an integrated resource for animal miRNA–target interactions. The validated targets component of this resource hosts a large, high-quality manually curated database of experimentally validated miRNA–target interactions with systematic documentation of experimental support for each interaction. The current release of this database includes 1135 records of validated miRNA–target interactions between 301 miRNAs and 902 target genes in seven animal species.
LC Sciences - microRNA Detection Microarray Service - miRBase History : miRBase Version 14.0 - The microRNA Sequence Database (miRBase) has now topped 10,000 entries. This latest release is miRBase version 14.0 and features a total of 10,581 mature miRNA sequences. The number of verified microRNAs has increased from 9,539 to 10,883 hairpin precursor miRNAs in 115 species. Many sequences have been updated or revised. New sequences added to: Model Species - Human (15), Mouse (32), Rat (39), Cow (259), Zebrafish (24), Drosophila (5), Silkworm (36) , C. elegans (19), Arabidopsis (3) Important Agricultural Species (280 new miRs) - Arabidopsis, Soybean, Rice, Maize, Sorghum, M. truncatula Viruses (30 new miRs) For the first time, miRNAs are reported from: Horse (346 miRs) First Crustacean - Daphnia 5 new plant species – including Apple and Green Bean 3 new Herpes virus species
LC Sciences - microRNA Microarray Service - Human Array Content : Human Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Mouse Array Content : Mouse Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Rat Array Content : Rat Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Zebrafish Array Content : Zebrafish Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Chicken Array Content : Chicken Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Fruit Fly Array Content : Fruit Fly Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Soil Nematode Array Content : Soil Nematode Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Rice Array Content : Rice Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Maize Array Content : Maize Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Arabidopsis Array Content : Arabidopsis Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Cow Array Content : Cow Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Human / Mouse / Rat Array Content : Human / Mouse / Rat Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Human / Viruses Array Content : Human / Viruses Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Mammals Array Content : Mammals Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Rodents Array Content : Rodents Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Vertebrates Array Content : Vertebrates Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Primates Array Content : Primates Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Insects Array Content : Insects Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Nematodes Array Content : Nematodes Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Fishes Array Content : Fishes Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Plants Array Content : Plants Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Viruses Array Content : Viruses Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Frogs Array Content : Frogs Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - Small Non-Coding RNA Discovery Microarray Service - Application Note : Recently, researchers at Baylor College of Medicine employed a discovery strategy of computational prediction and microarray expression profiling to uncover a large number of novel small RNAs in embryonic stem (ES) cells4. Dr. Preethi Gunaratne and co-workers reported using computation, custom small RNA microarray from LC Sciences, and validation experiments to uncover hundreds of novel small RNAs in ES cells. These small non-coding RNAs (ncRNAs) were from adult tissue progenitor transcript sequences and are micro-conserved elements; the detection probes were designed for custom array synthesis (www.lcsciences.com). In addition to the standard miRNA in miRBase, a comprehensive panel of 2,617 micro-conserved ncRNA sequences and several hundreds of literature reported small RNAs or miRNAs. Two generations of custom array designs were used for optimization the probes. The profiling studies using ES cell lines as well as normal adult mouse tissues identified 545 small RNAs that are enriched in ES cells over adult cells. Of these, more than 100 exhibited down-regulation in ES gene-knockout cells typical of microRNA.
LC Sciences - microRNA Microarray Service - Dual Sample Assays :
LC Sciences - microRNA Microarray Service - Dual Sample Assays :
LC Sciences - microRNA Microarray Service - Dual Sample Assays :
LC Sciences - Kinase Profiling Microarray Service - Detail : LC Sciences provides a comprehensive kinase analysis service utilizing high density protein kinase substrate (PKS) peptide microarrays synthesized on µParaflo® microfluidic chips for proteomic scale kinase profiling, quantitative measurement of kinase kinetic activities, and drug discovery research. This is a comprehensive kinase analysis service. Just send us your kinase sample (or we can order from a supplier) and we’ll perform all the necessary functions from sample assay to data analysis. Our Kinase Profiling comprehensive service includes: Synthesis of a standard content or custom designed protein kinase substrate (PKS) - peptide microarray on a µParaflo® microfluidics chip - Pre-staining the chip to assess synthesis quality and obtain baseline signal before the kinase sample is applied - Quality testing of the received kinase sample - On-chip enzymatic reactions - Specific staining of the phosphate group only on the phosphopeptides by fluorescent dye - Array image scan and data extraction - Data analysis - Basic data analysis (background subtraction, control and reference signal guided data processing, list of detected signals, data averaging, results summarized in a report) is included with our array service. Optional in-depth analysis based on your specific application is available.
LC Sciences - Protein Binding Microarray Service - Details : LC Sciences provides a comprehensive protein and/or antibody analysis service utilizing high density protein binding peptide microarrays for high throughput detection, concentration titration, and screening applications. This is a comprehensive analysis service. Just send us your protein/antibody sample and we’ll perform all the necessary functions from sample assay to data analysis. Our comprehensive service includes: - Synthesis of a custom designed protein binding - peptide microarray on a µParaflo® microfluidics chip - Pre-staining the chip to assess synthesis quality and obtain baseline signal before the protein sample is applied - On-chip binding reactions - Detection of the bound protein using one of several possible detection schemes - Array image scan and data extraction - Data analysis - Basic data analysis (background subtraction, control and reference signal guided data processing, list of detected signals, data averaging, results summarized in a report) is included with our array service. Optional in-depth analysis based on your specific application is available.
LC Sciences - Phosphopeptide Binding Peptide Microarray Service - Product Literature : Product Brochure - Peptide Microarray Service - Product Flyer - Biomarker Screening, Discovery & Detection Microarray Service - Technical Bulletin - Massively parallel peptide synthesis on a microchip based on the µParaflo® microfluidics technology
LC Sciences - Phosphopeptide Binding Peptide Microarray Service - Application Notes : Application Notes - SHP2 and SH2 Proteins Assayed on a Phosphopeptide Microarray Phosphopeptide Binding - Peptide Microarray - Technology Workshop
LC Sciences - Protein Binding Microarray Service - Pricing : Comprehensive Services Pricing - Phosphopeptide Binding - Comprehensive Service PPAS-1001 - Phosphopeptide Microarray Pricing - Custom Peptide Microarray PPA-2001 - Additional Peptide Synthesis (length beyond 8mer) PA-3001 - Custom Peptide Microarray Design Assistance PAS-1009 - In-Depth Data Analysis PAS-1007 - Quotes - If you are unsure about your particular service needs, please make use of our Peptide Microarray Service - Project Description Form.
LC Sciences - Epitope Mapping Peptide Microarray Service - Ordering : Please follow the four steps outlined below to place your order for a Epitope Mapping Peptide Microarray Service. Define the details of your experiments. Download a copy of our Project Description Form. Execute a service agreement. Complete a sample submission form prior to sending your sample to us. Send us your sample - Please see our protein sample submission page for sample requirements and instructions for shipping us your sample.
LC Sciences - Epitope Mapping Peptide Microarray Service - Details : LC Sciences now offers a comprehensive epitope mapping service for high throughput, high-resolution identification of epitopes and other protein-protein interactions. This is a comprehensive epitope mapping service. Just send us your protein/antibody sample and we’ll perform all the necessary functions from sample assay to data analysis.
LC Sciences - Epitope Mapping Peptide Microarray Service - Product Literature : Product Flyer - Epitope Mapping Microarray Service - Product Flyer - Biomarker Screening, Discovery & Detection Microarray Service - Technical Bulletin - µParaflo® Microfluidics Synthesis Technology - Technical Bulletin - Massively parallel peptide synthesis on a microchip based on the µParaflo® microfluidics technology
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes : Quantitative Measurement of AFM2 - Epitope Peptide Binding Quantitative Measurement of Anti HA Epitope Peptide Binding - Anti HA Binding: Association Curves Measured with Protein Concentration Titration on Epitope Peptide Chip - Anti HA Binding: Association Curves Measured with Peptide Concentration (Density) Variation by in situ Synthesis of Epitope Peptide Chip - Quantitative Measurement of Anti c-myc Epitope Peptide Binding - Quantitative Measurement of Anti VSVG Epitope Peptide Binding
LC Sciences - Epitope Mapping Peptide Microarray Service - Frequently Asked Questions : Frequently Asked Questions - General - What are epitope mapping - peptide microarrays? - Microarray Design - Sample Preparation - Sample Submission - Array Results - Data Analysis - Additional Questions
LC Sciences - Epitope Mapping Peptide Microarray Service - Pricing : Comprehensive Services Pricing Epitope Mapping - Comprehensive Service EAS-1001 Epitope Peptide Microarray Pricing Custom Peptide Microarray EPA-2001 Additional Peptide Synthesis (length beyond 8mer)PA-3001 Additional Services Pricing Custom Peptide Microarray Design Assistance PAS-1009 In-Depth Data Analysis PAS-1007 Quotes - If you are unsure about your particular service needs, please make use of our Peptide Microarray Service - Project Description Form.
LC Sciences - Careers - Technical Customer Service : LC Sciences current position openings - Technical Customer Service - Inside Sales
LC Sciences - microRNA Microarray Service - Application Note - MicroRNA Profiling & Molecular Circuitry : The mechanism of regulation by microRNA (miRNA) is complex in that miRNAs are frequently transcribed together as primary transcripts that are then processed into multiple individual mature miRNAs and, that each individual miRNA may target many different mRNAs. Thus miRNA represents a network of control over cellular functions. Additionally, the organization of many clusters of miRNAs is highly conserved and this suggests an important role for coordinated programmable function. Expression profiling of complete “microRNA-omes” enables researchers to look at this network on a broad scale and better understand the interrelationship of factors at work.
LC Sciences - microRNA Microarray Service - Application Note - MicroRNA Discovery and Validation : The study of microRNA (miRNA) is growing rapidly as researchers discover new miRNAs and uncover the importance of these small regulatory elements linked to a wide range of biological functions. The miRBase sequence databasev is the primary public repository for newly discovered miRNAs and the number of miRBase entries has grown rapidly from a mere 218 in 2002 to almost 10,000 in the latest version, suggesting the existence of many more miRNAs yet to be discovered.
LC Sciences - microRNA Sequencing Service - Application Notes - MicroRNA Discovery and Validation : The study of microRNA (miRNA) is growing rapidly as researchers discover new miRNAs and uncover the importance of these small regulatory elements linked to a wide range of biological functions. The miRBase sequence database is the primary public repository for newly discovered miRNAs and the number of miRBase entries has grown rapidly from a mere 218 in 2002 to almost 10,000 in the latest version, suggesting the existence of many more miRNAs yet to be discovered.
LC Sciences - Peptide Microarray Service - Picoliter Scale Titer Plates - Kinetic Studies : Quantitative Measurement of Kinase Kinetic Activities Including PKA Studies, Kinetic PKA kinase studies on a pico-liter titrator microarray, Make parallel measurements of reaction curves as a function of time and substrate concentration for determination of VMAX and KM.
LC Sciences - Peptide Microarray Service - Picoliter Scale Titer Plates - Assoc Curves : Anti HA Binding: Association curves measured with peptide concentration (density) variation by in situ synthesis of Epitope Peptide Chip. On chip measurements of anti HA binding to epitope YPYDVPDYA. Measured a 31-fold change in anti-HA binding for highest density vs regular peptide density.
LC Sciences - Phosphopeptide Binding Peptide Microarray Service - Application Notes - SHP2 and SH2 Proteins Assayed on a Phosphopeptide Microarray : Chip Design - More than 940 sequences plus controls and reference sequences - Typical Variations: EPIXAKVNKKVN X=pY, Y, A and possibly other amino acids, length variant sequences. - >3 redundancy - Assay - 0.2 µM protein - TBST buffer - Room Temp - Reaction time: 2 hours - Detection - Cy5-IgG - Results - Figure 1 - 3 Fluorescent images (inverted) of 4K peptide microarray after protein binding
LC Sciences - Phosphopeptide Binding - Peptide Microarray Service - Technology Workshop : Phosphoprotein binding proteins (PPBP) contain domains which binds to phosphorylation sites in phosphoprotein or phosphopeptide (PPEP).? LC Sciences provides a straightforward, high-throughput method for obtaining quantitative measurements of phosphoprotein activities. Through the use of high density arrays (PepArray?) with addressable ?signature? peptides for specific kinds of protein binding domains we can systematically screen thousands of sequences in a single experiment. A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events.
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - Quantitative Measurement of AFM2 Epitope Peptide Binding : Quantitative Measurement of AFM2 Epitope Peptide Binding - Chip Design - Epitope (known) DYKDDDDK - 312 Epitope and variant sequences - >4 redundancy - CV < 15% - Assay - Antibody: anti-flag peptide (AFM2) (1 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy3 - Results - Figure 1 - Fluorescent image of 4K peptide chip from AFM2 binding
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - Quantitative Measurement of Anti HA Epitope Peptide Binding : Quantitative Measurement of Anti HA Epitope Peptide Binding - Chip Design - Epitope (known) YPYDVPDYA - Chip (1) 800 HA and flag epitope and variant sequences - >3 redundancy - CV < 15% - Chip (2) HA, c-myc, VSVG, and flag epitope and other peptide sequences - >3 redundancy - CV < 30% - Assay - Antibody: anti-HA (100-1,000 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy5-IgG - Results - Figure 1 - Fluorescent image of Anti HA binding to epitope and its variants
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - Anti HA Binding: Association curves measured with protein concentration titration on Epitope Peptide chip : Anti HA Binding: Association curves measured with protein concentration titration on Epitope Peptide chip - Chip Design - Epitope (known) YPYDVPDYA - N-truncation - C-truncation - DYK plus N-truncation - DYK plus C-truncation - Assay - Antibody: anti-HA (0.1-60,000 ng/ml) - Detection - Signal intensities acquired at different scanning gains were scaled according to calibration curve. Curve fitting used Origin (Origin Lab). - Results - Figure 1 -Plots of association curves.
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - Anti HA Binding: Association curves measured with peptide concentration (density) variation by in situ synthesis of Epitope Peptide Chip : Anti HA Binding: Association curves measured with peptide concentration (density) variation by in situ synthesis of Epitope Peptide Chip. - On chip measurements of anti HA binding to epitope YPYDVPDYA. - Illustration of a method for high throughput measurements of protein-peptide interactions. - Chip Design - Epitope (known) YPYDVPDYA - N-truncation - C-truncation - DYK plus N-truncation - DYK plus C-truncation - Assay - Antibody: anti-HA (100 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Detection through secondary antibody with dye(cy3 or cy5) conjugation (100 mg/ml) - Results - Figure 1 - Image of the chip and plot of the chip data.
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - Quantitative Measurement of Anti c-myc Epitope Peptide Binding : Quantitative Measurement of Anti c-myc Epitope Peptide Binding - Chip Design - Epitope (known) EQKLISEEDL - HA, c-myc, VSVG, and flag epitope and other peptide sequences (see figure 3 below for detail) - >3 redundancy - CV < 30% - Assay - Antibody: anti-c-myc (1 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy5-IgG (100ng/ml) - Results - Figure 1 - Fluorescent images of 4K peptide chip for 3 different experiments
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - Quantitative Measurement of Anti VSVG Epitope Peptide Binding : Quantitative Measurement of Anti VSVG Epitope Peptide Binding - Chip Design - Epitope (known) YTDIEMNRLGK - HA, c-myc, VSVG, and flag epitope and other peptide sequences (see figure 3 below for detail) - >3 redundancy - CV < 30% - Assay - Antibody: anti-VSVG(1 ng/ml) - 200 µl TBS - Temp: 4oC - Reaction time: 1 hour - Detection - Cy5-IgG (100ng/ml) - Results - Figure 1 - Fluorescent image and detail of chip layout
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LC Sciences - Specialty Proteomics Microarray Service - Application Notes - App 1 Fig 1 :
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LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - App 6 Fig 1 :
LC Sciences - Epitope Mapping Peptide Microarray Service - Application Notes - App 6 Fig 2 :
LC Sciences - DNA/RNA Aptamer Microarray Service - Ordering : Please follow the four steps outlined below to place your order for a DNA/RNA Aptamer Binding Microarray Service. Define the details of your experiments. Download a copy of our Project Description Form. Complete as much of this form as possible. We will use the information you provide to design your experiments. Note - for items left blank we will insert information based on our successful experiences. Email the form to orders@lcsciences.com. Customer service will contact you to finalize the details of your project. Execute a service agreement. When the details have been finalized we will send you a service agreement and a quote. Sign the service agreement and FAX it back to us. (713) 664-8181. Use the quote to obtain a purchase order from your institution. Complete a sample submission form prior to sending your sample to us. In order to ensure a successful experiment, we need to know as much about your sample as you can tell us. The form can be emailed to us at: orders@lcsciences.com beforehand or sent in the package along with your sample. Note - If you send the form in the package with samples please place it in a separate waterproof bag. Send us your sample
LC Sciences - DNA/RNA Aptamer Microarray Service - Scientific Publications : Application Articles (Aptamer Oligonucleotide Microarrays)
LC Sciences - DNA/RNA Aptamer Microarray Service - Application Notes : Discovery of Protein-Binding DNA Sequences on an Aptamer Oligonucleotide Microarray - Mapping a specific binding sequence of an aptamer oligonucleotide to decrease the molecule size for many practical applications such as drug molecules - Other Applications - Protein-Nucleic Acid Binding - Drug Screening - Target Validation - Biosensor Screening - Biomarker Screening - Diagnostic Chip Development - Inhibitor Assays -
LC Sciences - DNA/RNA Aptamer Microarray Service - Available Arrays : Standard Array - A predesigned microarray built on a µParaflo® microfluidics chip. Our standard aptamer microarray includes >1000 known DNA aptamers. DNA, 2'-OMe RNA, and 2'-F RNA synthesis chemistries are available. Complete list of standard content - Custom Array - A custom microarray containing aptamer sequences determined by the customer built on a µParaflo® microfluidics chip. We can synthesize a completely custom microarray designed specifically for your experiment. Our basic chip design has 4K features. DNA, 2'-OMe RNA, and 2'-F RNA synthesis chemistries are available. Contact us for assistance with design of your DNA/RNA aptamer microarray.
LC Sciences - DNA/RNA Aptamer Microarray Service - Pricing : Standard Array Content Option - All standard aptamer microarrays are synthesized on a µParaflo® microfluidics chip >1000 standard aptamer sequences (see probe content) - Aptamer Microarray - Standard (DNA)AA-1001 - Aptamer Microarray - Standard (2'-OMe RNA)AA-1002 - Custom Array Content Option - Synthesis of a custom designed DNA/RNA aptamer microarray on a µParaflo® microfluidics chip, length up to 25mer. Up to 3,918 customer defined sequences - Customizable features incvlude - sequence design, varying lengths, chip layout, synthesis chemistry, and more! - Aptamer Microarray - Custom (DNA)AA-2001 - Aptamer Microarray - Custom (2'-OMe RNA)AA-2002 - Additional DNA/RNA Synthesis (length beyond 25mer) - Synthesis of additional DNA bases DA-3001 - Synthesis of additional 2'-OMe bases RA-3002 - Additional cost only applies to bases beyond 25mer.
http://www.lcsciences.com/documents/sample_data/in_depth_S70xxx_multiarray_normalized%20_data_sample.xls :
LC Sciences - DNA/RNA Aptamer Microarray Service - Application Notes - Discovery of Protein-Binding DNA Sequences on an Aptamer Oligonucleotide Microarray : Discovery of Protein-Binding DNA Sequences on an Aptamer Oligonucleotide Microarray - Identify specific sequences. Differentiate single strand vs. duplex binding. Identify high definition patterns of the binding. Chip Design - Contains both single stranded and duplex oligonucleotides - Varying sequence lengths - Assay - Sample - CUGBP1 is a Myotonic Dystrophy (DM1) related protein - Detection - Anti-His and Cy3 IgG - Results - Figure 1 - Schematic of detection with secondary antibody on aptamer microarray
LC Sciences - DNA/RNA Aptamer Microarray Service - Application Notes - Mapping a specific binding sequence of an aptamer oligonucleotide : Mapping a specific binding sequence of an aptamer oligonucleotide to decrease the molecule size for many practical applications such as drug molecules - Identification of key positions of a consensus sequence for enhancing binding affinity (possibly by modifying those positions) for an aptamer drug candidate. Through this strategy, we can map a small but highly specific binding aptamer oligonucleotide. Background - Macugen (pegaptanib sodium) is an ophthalmic aptamer drug being developed by Pfizer/Eyetech for the treatment of the 'wet' forms of age-related macular degeneration (AMD). The consensus sequence is CGGAAUCAGTGAATGCTTATACATCCGt, an RNA 28 mer where all pyrimidines are modified with a 2'-F and the all the purines (except 2 As) are modified with a 2'-'OMe group. Our aptamer chip is a powerful tool for further refining the consensus sequences identified from the in vitro evolution (SELEX), by finding the minimal length of oligonucleotide required for specific binding (core sequence) and systematic modification of the core sequence. The above example shows a strategy for searching the region of core sequence, which will show highest binding signals.
LC Sciences - DNA/RNA Aptamer Microarray Service - Application Notes - Fig 1 :
LC Sciences - DNA/RNA Aptamer Microarray Service - Application Notes - Fig 3 :
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LC Sciences - Company News & Events 2010 : January 11 - 16, 2009 - San Diego, CA - PepTalk - The Definitive Protein Focused Event - We invite you to visit us at booth #13 to learn more about our custom peptide microarray products and services for kinase substrate profiling, epitope mapping, and phosphopeptide-binding protein studies. Dr. Christoph Eicken (Head of Technical Services, Microarrays) will be presenting a poster: Proteomic Kinase Substrate and Phosphopeptide Chip Technology for Protein Profiling - Although a number of proteomics technologies are well-developed, the demand for reliable, sensitive, accurate, and comprehensive measurements of protein expression remains unfulfilled. Our work is based on using a) protein kinase (PK) phosphorylation activities and b) phosphopeptide-phosphoprotein binding domain (PPEP-PPBD) interactions to detect protein in complex biological samples. We have a novel, in situ, on-microchip synthesis technology (µParaflo®) that allows us to construct in situ synthesis of high-density peptide arrays according to custom designs. We also apply this flexible peptide array technology for rapid and reliable quantitative measurements for phosphorylation and protein binding – 41 96-well titer plate experiments (i.e. conventional experiments) can be accomplished simultaneously on one chip. Our ultimate goal is to provide new, powerful research and clinical study tools to address the critical needs in proteomic research, clinical diagnosis, and therapeutic treatment.
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LC Sciences - microRNA Microarray Service - Application Note - Integrating microRNA and mRNA expression profiles : microRNA profiling from Blood / Plasma Samples. The regulatory effects microRNA of are widespread and microRNAs have recently been implicated in various diseases of the blood, stem cells, brain, and other tissues. MicroRNAs are expressed in the blood. A study involving sickle cell diseases revealed that there is significant microRNA expression in red blood cells and another showed that microRNAs are highly expressed in the ischemic brain and can be detected in blood samples.
LC Sciences - microRNA Microarray Service - Rice Array Content : Rice Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Maize Array Content : Maize Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Arabidopsis Array Content : Arabidopsis Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Wheat Array Content : Wheat Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
LC Sciences - microRNA Microarray Service - Plants Array Content : Plants Array Content - Microarrays designed for genome-wide microRNA expression profiling and built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform . These microarrays are available as part of our comprehensive microRNA Expression Profiling Service.
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