Our comprehensive sequencing services make use of the latest in next-gen sequencing technologies. Illumina’s industry-leading RNA sequencing methods enable discovery and profiling of RNAs in any organism without prior genome annotation, and allow for the most accurate detection and quantification of rare RNA sequences and sequence variants in your samples.
- Advanced RNA-Seq Technology – Enables transcriptome profiling of any species. There is no need for design of probes that must be based on prior sequence or secondary structure information, therefore profiling is truly genome-wide. Achieve “digital” transcript expression analysis over a potentially unlimited dynamic range. Reveal sequence variations in the transcripts due to alternative splicing events and provide allele-specific or isoform-specific gene expression.
- Turnkey Solution – These comprehensive services are designed to be one-stop and produce the results needed to quickly advance your biological and biomedical research. Our “Total RNA to Data” services include: sample prep/QC, library preparation, RNA sequencing, bioinformatics analysis, and high-level customer support.
- Expert Services – LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into RNA research. We don’t merely deliver data; we will handle all the advanced bioinformatics analysis and help you find answers to the questions you’re asking.
- Next-Gen Microarrays – Our ability to combine custom next-gen microarray profiling with discovery sequencing can leverage both technologies and capture more information from your sequencing datasets.
Next-gen sequencing is a new method and powerful tool used to identify and quantitatively decode the entire population of small RNAs in your total RNA sample. LC Sciences provides a comprehensive discovery sequencing service utilizing Illumina’s high-throughput sequencing technology which enables comprehensive, highly sensitive and specific discovery and profiling of all forms of small RNAs in your sample.
Degradome sequencing (Degradome-Seq), also referred to as parallel analysis of RNA ends (PARE), a modified 5′-rapid amplification of cDNA ends (RACE) with high-throughput next-gen sequencing method. Degradome sequencing provides a comprehensive means of analyzing patterns of RNA degradation. Degradome sequencing has been use to identify microRNA (miRNA) cleavage sites, because miRNAs can cause endonucleolytic cleavage of mRNA by extensive and often perfect complementarity to mRNAs. Degradome sequencing has revealed many known and novel plant miRNA (siRNA) targets.
The incredible power of RNA-Seq was initially applied primarily for complex whole transcriptome applications such as discovery of rare genes, splice junctions and gene fusions or for study of novel and less well-characterized organisms. However, as the technology is improving and costs are decreasing, RNA sequencing is now suitable to be used for simple RNA profiling (sample comparison) as well. RNA-Seq in fact holds several advantages over microarrays for gene expression analysis applications.
RNA-Seq technology is providing fresh biological insight into the transcriptome. At its most powerful, it can be used to determine the structure of genes, their splicing patterns and other post transcriptional modifications, to detect rare and novel transcripts, and to quantify the changing expression levels of each transcript. LC Sciences provides a turnkey solution for transcriptome sequencing featuring the latest in RNA-Seq technology.
Get more from your sequencing datasets! Microarrays are proven, effective tools to systematically profile and compare gene expression rapidly, reproducibly, and cost effectively. We use custom microarrays with content based on your comprehensive sequencing findings to capture more information from your data.