Kinase Profiling Microarray Service

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Kinases are key components of cell signaling pathways by transfer of the terminal phosphate molecule from ATP to the serine, threonine, or tyrosine residues of a protein. Through the use of peptides as specific kinase substrates, we can make quantitative measurements of kinase activities and profiling kinases on a peptide microarray offers the opportunity to study thousands (up to thousands of peptides/chip) of specific substrates in a single experiment.

LC Sciences provides a comprehensive kinase analysis service utilizing high density protein kinase substrate (PKS) peptide microarrays synthesized on µParaflo^® microfluidic chips for proteomic scale kinase profiling, quantitative measurement of kinase kinetic activities, and drug discovery research. This comprehensive service includes preparation of your kinase sample, single or dual color enzymatic reactions, array scanning and data analysis. Two-three weeks after receiving your kinase sample(s), we send you a data summary report containing background subtraction, control and reference signal guided data processing, list of detected signals, and data averaging. We offer additional services for in-depth kinetic data analysis and curve analysis of quantitative measurements.

We have a comprehensive set of bioinformatics tools and extensive experience in microarray work. We will work with you from peptide selection, array design, experiment, to data analysis, and we understand that what you need is to obtain reliable and rich information that is not obtainable with a single kinase assay or even a 96-well format assay.

Comprehensive Service

This is a comprehensive kinase analysis service. Just send us your kinase sample (or we can order from a supplier) and we’ll perform all the necessary functions from sample assay to data analysis. Our Kinase Profiling comprehensive service includes: Synthesis of a protein kinase substrate (PKS) - peptide microarray, pre-staining the chip to assess synthesis quality , on-chip enzymatic reactions, specific staining of the phosphate group only on the phosphopeptides by fluorescent dye, array image scan and data extraction, and data analysis.

High Throughput Microarray Format

Traditional kinase assays focus on a single to a few peptide or protein substrates. Profiling kinases on a peptide microarray offers the opportunity to study thousands (up to thousands of peptides/chip) of specific substrates in a single experiment. One experiment using our protein kinase substrate (PKS) - peptide microarray is equivalent to 41 experiments using a 96-well plate.

Custom Microarray Content

Customize one of our standard microarrays or design your own PKS- Peptide Microarray. We can synthesize a completely custom microarray specifically for your experiment. Our basic format is 3,918 different 8-mer peptides per chip but additional length is possible. Ask us for assistance with design of your PKS- Peptide Microarray.

Quantitative Results

All assays use universal, highly phosphate-specific fluorescent dye staining - two color experiments are possible. Well-designed negative and positive control references ensure reliable and quantifiable results. In situ synthesized PKS - peptide microarrays using our µParaflo^® microfluidic chip technology deliver more uniform spots and produce a more reliable reading of the assay signals. Multiple replicates of each peptide sequence also contributes to data confidence.

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