Protein Binding Microarray Service - Application Note

Quantitative Measurement of Anti VSVG Epitope Peptide Binding

Chip Design

• Epitope (known) YTDIEMNRLGK
• HA, c-myc, VSVG, and flag epitope and other peptide sequences (see figure 3 below for detail)
• >3 redundancy
• CV < 30%

Assay

• Antibody: anti-VSVG(1 ng/ml)
• 200 µl TBS
• Temp: 4^oC
• Reaction time: 1 hour

Detection

• Cy5-IgG (100ng/ml)

Results

• Figure 1 - Fluorescent image and detail of chip layout

Figure 1

• Figure 2 - Plot of the signal intensities of the epitope sequence variants

Figure 2

• Figure 3 - Chip sequence information detail

Figure 3

Sitemap • Privacy • Terms of Use • Disclaimer

Copyright © 2008, LC Sciences