Protein Binding Microarray Service - Application Note

Anti HA Binding: Association curves measured with protein concentration titration on Epitope Peptide chip

Chip Design

• Epitope (known) YPYDVPDYA
• N-truncation
• C-truncation
• DYK plus N-truncation
• DYK plus C-truncation

Assay

• Antibody: anti-HA (0.1-60,000 ng/ml)

Detection

• Signal intensities acquired at different scanning gains were scaled according to calibration curve.
• Curve fitting used Origin (Origin Lab).

Results

• Figure 1 -Plots of association curves.

Figure 1

K_d values measured are in the range of 2-4 µg/ml

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