Protein - Aptamer Binding Microarray Service

Discovery of Protein-Binding DNA Sequences on an Aptamer Oligonucleotide Microarray

• Identify specific sequences.
• Differentiate single strand vs. duplex binding.
• Identify high definition patterns of the binding.

Chip Design

• Contains both single stranded and duplex oligonucleotides
• Varying sequence lengths

Assay

• Sample - CUGBP1 is a Myotonic Dystrophy (DM1) related protein

Detection

• Anti-His and Cy3 IgG

Results

• Figure 1 - Schematic of detection with secondary antibody on aptamer microarray

Figure 1

• Figure 2 - A commonly accepted RNA-binding protein found to be DNA-binding as well.

Figure 2

• Figure 3 - Fluorescent image (inverted) of 4K aptamer binding microarray

Figure 3

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