Small RNA Discovery & Analysis - Sequencing Service

Small RNAs make up a large family of regulatory molecules in organisms of nearly all kingdoms of life.  These include: miRNA, ncRNA, siRNA, snoRNA, piRNA, rasiRNA, and many more.  Their functions are equally as diverse, including mRNA degradation, translational repression, hetero- chromatin formation, and DNA elimination as they play an important role in development and disease.  Small RNA transcriptome sequencing is a new method and a powerful tool to identify and quantitatively decode the entire population of small RNAs and thus recover the families of regulatory RNAs in different organisms and cellular systems.

Small RNA Sequencing

• Profile small RNA in any species - there is no need for probes based on prior sequence or secondary structure information.
• Profiling is transcriptome-wide - investigate all small RNAs, of any size, known and unknown, in your sample.
• Achieve digital transcript expression analysis - Accurate quantitation of expression levels over 5 orders of magnitude of transcript abundance.
• Detect rare transcripts and transcript variants, such as single nucleotide mutations (SNP).

Our Comprehensive Service

This service is comprehensive - from sample to data, providing advanced technology and years of experience in efficient discovery and profiling of small RNAs.

Our “Total RNA to Data” comprehensive service includes: sample QC, sample preparation (small RNA selection,  library preparation, single molecule clonal amplification), high-throughput sequencing, bioinformatics analysis, and customer data report.

Optional target-specific selection service is available to enrich your sample(s) for more focused sequencing to achieve deeper coverage.

Down-stream profiling of identified (and other customer defined target small RNAs) is also available by custom microarray analysis service.

Data Analysis / Results

• Standard Solexa pipeline data analysis is included.
• Additional LC Sciences software processing is included.
• Data is filtered and reduced to mappable reads.

For each sample analyzed, the customer receives:

• Either FASTA-A or FASTA-Q files with base-calling quality scores.
• A list of detected sequences.
• Copy number per sequence.

Additional in-depth analysis is available:

• Map to reference sequence - Sequence data can be aligned to publicly available small RNA sequence databases.
• Differential expression analysis for multiple sample experiments - t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.

Sitemap • Privacy • Terms of Use • Disclaimer

Copyright © 2009, LC Sciences