Small Non-coding RNA Discovery - Microarray Service

Customer Publications

1  Vagin VV, Sigova A, Li C, Seitz H, Gvozdev V, Zamore PD. (2006) A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline. Science 313(5785), 320-324.  [abstract]

2  Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA Jr, Sjoblom T, Barad, O, Bentwich Z, Szafranska AE, Labourier E et al. (2006) The colorectal microRNAome. Proc Natl Acad Sci U S A 103, 3687-3692.  [abstract]

Non-coding  (ncRNAs) are a class of RNAs that do not encode proteins but instead possess regulatory function at the level of RNA in the cell. Custom microarrays have been shown to be a effective experimental approach to discovery of novel ncRNAs¹ as well as a method to validate candidate ncRNAs². LC Sciences provides a small non-coding RNA discovery service using innovative µParaflo^® technology and proprietary probe design, which enable highly sensitive and specific direct detection of small RNAs in your sample. This service is comprehensive; from array design to sample to data, allowing the most efficient novel discovery of small regulatory/functional RNA embedded within non-coding RNAs (ncRNAs) or genomic RNAs.

Comprehensive Service - Send us your total RNA sample and we’ll perform all the necessary functions to complete your microarray experiment including: sample QC, sample preparation (small RNA enrichment and labeling), microarray hybridization, array scanning and data extraction, and data analysis.

Completely custom probe content  - allows you to design an array cover your specific area of interest in the genome of your species of choice.  These are not spotted arrays.  Utilizing our flexible microfluidic on-chip synthesis technology, we will custom synthesize your microarray to order when you order it.

Direct labeling of RNA  - eliminates the potential bias involved with transcription of sample(s).

Fast service- In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences,  raw and processed data, and a summary of the results.

Assay performance - A microarray system sensitive enough to detect low abundance sequences and specific enough to differentiate single base mismatch from perfect match sequences.

Microarray Platform - µParaflo^® microfluidic chip technology enables us to achieve such high quality and product reliability.

This is the same exact microarray service used for detection of microRNA.

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