microRNA qRT-PCR Services

LC Sciences now provides a one-stop solution for all your microRNA (miRNA) expression profiling needs.  We can provide you with both microarray and qRT-PCR profiling services for the most complete picture of microRNA expression in your samples.  While microarrays provide efficient genome-wide screening of miRNA expression, qRT-PCR is the gold standard for validation of microarray expression data and provides quantitative analysis of microRNA expression in real time which can help you to zero in on the miRs of interest in your samples revealed by microarrays.  In addition, the specificity of real time PCR expression data can provide further understanding of the true biological significance of these miRs.

Our service is comprehensive and includes small RNA enrichment from your total RNA sample, TaqMan^®microRNA assay (in triplicate), and fully analyzed quantitative data.

TaqMan^® MicroRNA Assays quantitate microRNAs with the specificity and sensitivity of TaqMan^® assay chemistry. The assays target only mature microRNAs, not their precursors, ensuring biologically relevant results.

Every TaqMan^® MicroRNA Assay has been functionally validated under laboratory conditions to ensure accurate results.

QUANTITATIVE analysis of microRNA expression for your RNA sample(s) in REAL TIME

• Comprehensive - This is a comprehensive service from your total RNA sample to fully analyzed quantitative microRNA expression data.
• Specific - These are highly sensitive validated assays specific to the miR(s) of your choice.
• 1-Stop Solution - qRT-PCR is the gold standard for validation of microarray expression data.  We can provide you with both microarray and qRT-PCR profiling services for the most complete picture of microRNA expression in your samples

RESULTS

• Relative Quantitation – For multiple sample experiments (e.g. treated and untreated) we can perform statistical analysis (t-test) to provide relative expression levels.
• Customer should suggest one or preferable two endogenous controls.  We will add an assay of each sample with the selected control probe(s) to the plate in order to calculate relative quantitation between samples.  t-test will be performed.
• Absolute Quantitation – We will add a standard curve (of spike in control RNA) to each plate.  There are 7 data points/curve.
• In all cases both raw and fully analyzed data will be provided to you.

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