|
|
|
 |
microRNA Microarray Service - Product News |
|
|
|||||||||||||
August 2, 2007 - Sanger miRBase microRNA Sequence Database Updated to Release 10.0The study of microRNA (miRNA) is a relatively new field with the first significant publications appearing in 2001. Our knowledge continues to grow rapidly as more and more researchers race to discover new miRNAs and uncover the importance of these small regulatory elements. Recent publications have linked miRNAs with a wide range of biological functions and as new miRNAs are discovered and experimentally verified, the data are deposited to the public database, miRBase. The miRBase sequence database1 is a comprehensive database of miRNA sequence data, annotation, and predicted gene targets and is the primary public repository for these data miRBase also provides a gene-naming service for assigning official miRNA names to novel miRNAs before they are published. miRBase provides a convenient, searchable online database, allowing the user to search by key word or sequence for information about known miRNAs and their targets and is freely available to all at http://microrna.sanger.ac.uk/. The Sanger Institute released the latest version (10.0) of their miRBase sequence database for known miRNAs today (http://microrna.sanger.ac.uk/sequences/) This is the third update in 2007 and there have been 22 updates since the initial release in late 2002. Updates occur whenever a significant number of experimentally verified sequences are added to the database. For this update, 487 new hairpin sequences have been added, with 492 novel mature miRNA products including the first entries for three new plant species. Release 10.0 of the database contains 5071 entries representing hairpin precursor miRNAs, expressing 4922 mature miRNA products, in primates, rodents, birds, fish, worms, flies, plants and viruses (miRBase release summary). Detection of miRNAs using a microarray offers the opportunity for genome-wide miRNA expression profiling by examining all known miRNA transcripts in a single experiment The public miRBase sequence database serves as the primary probe content for many commercially available miRNA profiling microarrays However, the continued updating of the database can be problematic when dealing with pre-spotted glass slide arrays as the probe content of the arrays immediately goes out of date whenever a new miRBase version is released. Researchers feel it is important to have the most complete picture of miRNAs expressed in their experimental samples. Existing spotted microarrays containing miRBase version as recent as 8.0 content are missing 38% of Human sequences, 39% of Mouse sequences, and 38% of plant sequences and it may take quite some time before spotted array probe content can be updated LC Sciences miRNA detection microarrays make use of a microfluidics on-chip synthesis platform, termed µParaflo®,versus a traditional spotted array based on pre-synthesized oligonucleotides. An on-chip synthesis platform solves the issue of out of date microarrays because made-to-order microarrays can be produced, delivering the most up-to-date research tools to researchers. In addition to providing much more uniform and reproducible features than a spotted array, on-chip synthesis also permits the total customization of content on each individual microarray opening up additional applications such as the discovery of new miRNAs and other small non-coding RNAs. About microRNA (miRNA) – miRNAs are small non-protein-coding RNA molecules that function as negative regulators of gene expression by base pairing with specific mRNAs. This either inhibits translation or promotes mRNA degradation. About miRBase - The miRBase sequence database is a comprehensive database of miRNA sequence data, annotation, and predicted gene targets and is the primary public repository for these data. miRBase also provides a gene-naming service for assigning official miRNA names to novel miRNAs before they are published. About LC Sciences - LC Sciences offers specialty microarray services for nucleic acid/protein profiling and functional analysis, biomarker-discovery, and novel drug screening. Our array service products are based on Atactic Technologies’ µParaflo®platform technologies that encompass advanced digital chemical synthesis, pico-liter scale biochemical assays, and microfluidic reaction devices containing high density individual 3D chambers. More information about LC Sciences is available at http://www.lcsciences.com.
|
|
Sitemap • Privacy • Terms of Use • Disclaimer |
Copyright © 2008, LC Sciences |