microRNA Microarray Service - Application Note

Characterization of breast cancer using microRNA expression profiling and epigenomic and proteomic measurements in rat model

“Estrogen-Induced Rat Breast Carcinogenesis is Characterized by Alterations in DNA Methylation, Histone Modifications and Aberrant MicroRNA Expression” by Kovalchuk and co-workers

One most important outcome of the current study is the first time demonstration of miRNA expression in mammary tissue correlates with epigenetic dysregulation manifested in morphological changes and alteration of DNA methylation and histone methylation. In particular, using miRNA microarray analysis (provided by LC Sciences) of the E2-induced carcinogenesis development in rat, the down‑regulated miR‑22, miR‑99a, miR‑99b, and miR‑127 are shown to correlate with elevated target oncogene activities.  In this series of studies of the three main components of epigenetics, the authors concluded that “Changes in DNA and histone methylation, and, importantly, deregulation in the miRNAome may lead to the emergence of epigenetically reprogrammed cells with a tumor‑specific phenotype (inhibition of cell cycle arrest and apoptosis, activation of cell proliferation) leading to subsequent malignant transformation”.

LC Sciences pioneered miRNA microarray service and continues to be the leader in providing the most current miRNA probe content for miRNA expression profiling.  We currently offer the only comprehensive microRNA microarray service available with probe content of all species from Release 10.0 of the miRBase sequence database. The service package provided by LC Sciences has been developed and extensively used in thousands of successful experiments for addressing the questions in miRNA research.  Our results are proven to be of highest quality, reliable, relevant to related biological studies. In addition to standard public released miRNAs, LC Sciences offers fully customizable microarray products for detection and profiling of other types of regulatory RNAs, including ncRNAs and viral RNAs.

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