microRNA Detection Microarray Service

Additional Information

Available Arrays

Customer Publications

Data

Recent Customer Publications

Gu P et al. (2008) Novel microRNA candidates and miRNA-mRNA pairs in embryonic stem (ES) cells. PLoS ONE 3(7), e2548.
[abstract]

Kovalchuk O et al. (2008) Involvement of microRNA-451 in resistance of the MCF-7 breast cancer cells to chemotherapeutic drug doxorubicin. Mol Cancer Ther 7(7), 2152-59. [abstract]

He PA et al. (2008) Identification and characteristics of microRNAs from Bombyx mori BMC Genomics 9(1), 248. [abstract]

Zhang J et al. (2008) Identification of conserved microRNAs and their targets from Solanum lycopersicum Mill. Gene [Epub ahead of print] [abstract]

Mellios N et al. (2008) A Set of Differentially Expressed miRNAs, including miR-30a-5p, act as Post-transcriptional Inhibitors of BDNF in Prefrontal Cortex. Hum Mol Genet [Epub ahead of print] [abstract]

Singh SK et al. (2008) REST maintains self-renewal and pluripotency of embryonic stem cells. Nature [Epub ahead of print] [abstract]

LC Sciences provides a genome-wide microRNA (miRNA) expression profiling service using µParaflo^®technology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs. We have standard arrays for mature miRNA of all species available in the latest version of the Sanger miRBase database (Release 12.0). Our service is comprehensive and includes small RNA enrichment from your total RNA sample, single or dual color labeling, hybridization, image data processing and data analysis. Two-three weeks after receiving your total RNA samples, we send you the analyzed data, representative and original images of the array, and raw data. We offer additional products and services including arrays containing miRNA*, walk/tiling arrays, custom arrays (first 100 probes free of charge), and in-depth analysis for multiple data sets. The optional data analysis may include multi-array normalization, t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.

Special Announcement - miRBase Version 12.0 - Now Available!

Sept 1st, 2008 - With the addition of 2062 new mature miRNA sequences, the Sanger microRNA Sequence Database (miRBase) announced it's largest update in miRBase history: the latest release is miRBase version 12.0 and features a total of 8273 mature miRNA sequences. Many sequences have been updated or revised. For the first time, miRNAs are reported from : 10 new Drosophila species, Platypus, Tomato, Red Flour Bettle, and Two polyomaviruses. There has been a significant increase in the number of miRNAs reported for Human and model species such as Chimpanzee, Mouse, and Arabidopsis as well as agriculturally important species such as Rice, Soybean, Pig, and Chicken.

Featuring µParaflo® Biochip Technology - A high performance microfluidic custom microarray platform.

100% miRBASE VERSION 12.0 CONTENT
COMPLETE CONTENT FLEXIBILITY (All Species)
OPTIMIZED RNA HYBRIDIZATION PROBES
EXPERIENCED SERVICE PROVIDER HANDLING VARIOUS RESEARCH & CLINICAL SAMPLE TYPES
(Including FFPE and Blood/Plasma Samples)
COST EFFECTIVE 1-STOP SOLUTION

Comprehensive Service Available	√
Microarray Platform	µParaflo^®Microfluidic Biochip Technology
Dual Color Capable	√
Starting Sample Material	Total RNA, miRNA, Blood, Cell Pellet, Tissue, FFPE
Sample Requirement	5 µg Total RNA*
Probe Content	miRBase 12.0 (Sept 2008)
Species Covered	All species for which data exists (in Sanger miRBase)
Probes T_m Equalized	√
Add Custom Sequences	√
Detection Limit	< 100 attomole
Dynamic Range	>3.5 logs
Full Data Analysis Included	√
Data Delivery Time	3-4 Weeks
Number of Customer Publications to Date	53

Latest Product News

Sept 1, 2008 - The Sanger Institute announces the largest single update in the history of miRBase with the of 2062 new mature miRNA sequences. In addition to sequence updates and additions to species included in previous miRBase versions, mature miRNA sequences from many new species are included for the first time. (read more...)

May 6, 2008 – LC Sciences announced today that it has entered into a strategic marketing alliance with DNAVision to increase accessibility to its custom microRNA profiling service and enhance both its customer and technical service to researchers in Europe. (read more...)

April, 2008 - The Sanger miRBase sequence database was updated to release version 11.0. The number of verified microRNAs has been increased from 5395 to 6396 hairpin precursor miRNAs expressing 6211 mature miRNA products. This latest microRNA sequence content is currently available on all our standard and custom microRNA microarrays. (read more...)

February, 2008 – LC Sciences tops 30th customer publication. LC Sciences announced today the publication of the 30th peer-reviewed study by one of its customers using the company’s microarray service for analyzing microRNA expression profiles and for discovery of novel small RNAs. These studies, by leading researchers in the field, contribute to a fast growing body of knowledge defining this recently discovered class of regulatory RNAs. (read more...)

December, 2007 - The Sanger miRBase sequence database was updated to release version 10.1. This marks the fourth update this year. The number of verified microRNAs has been increased from 5071 to 5395 hairpin precursor miRNAs expressing 5234 mature miRNA products. This latest microRNA sequence content is currently available on all our standard and custom microRNA microarrays. (read more...)

Overview of the Service

Microfluidics Microarray Platform

The µParaflo® technology integrates high throughput parallel synthesis, microfluidics, and digital photolithography to create custom arrays of DNA, RNA, or peptides. Our advanced microarray platform delivers more reliable results than are possible with traditional spotted arrays.

Optimized RNA Hybridization Probes

LC Sciences’ Optimized RNA Hybridization Probes are designed with normalized Tms to ensure uniform hybridization affinity to their microRNA targets under high stringency hybridization conditions enhancing both the sensitivity and specificity of the probes.

The Most Current Content

LC Sciences offers the only microRNA microarray currently available with version 12.0 probe content. Not 90% or 95% coverage, but 100% of experimentally verified microRNA sequences are represented on all of our arrays.

Complete Content Flexibility

All of LC Sciences’ microarrays are made to order and synthesized to each customer’s particular specifications. Validate predictions, add custom controls, or create totally custom tiling arrays for discovery of novel small-RNAs. The choice is up to you!

No Limitation on Use of Data

Unlike with proprietary probe content, we hold no claims on your data. You have full access and control over the results generated from your experiments.

The Standard for Array Data Quality

The µParaflo^® technology enables on-chip synthesis ensuring high probe quality and tight process control. The data obtained by these microarrays is highly reliable and reproducible as validated by qPCR and Northern Blot.

In Depth Data Analysis

LC Sciences’ in-depth data analysis includes all t-Tests, ANOVA and heat maps to get you the information you need. Complex multi-array experiments are no problem for our experienced technical team. We can easily compare samples from different chips or even new experimental data to data from previous experiments.

The Most Customer Publications

LC Sciences' customers have published over 30 papers to date; have confirmed that microRNAs play an important role in a wide array of biological functions.

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