microRNA Discovery Sequencing Service

LC Sciences now provides a one-stop solution for all your microRNA (miRNA) discovery and expression profiling needs.  High-throughput sequencing is now available in addition to existing microarray and qRT-PCR profiling services for the most complete picture of microRNA expression in your samples.  microRNA sequencing is a new method and a powerful tool to identify and quantitatively decode the entire population of microRNAs in your sample. 

microRNA Sequencing

• Profile microRNA in any species - there is no need for probes based on prior sequence or secondary structure information.
• Profiling is transcriptome-wide - investigate all microRNAs, of any size, known and unknown, in your sample.
• Achieve digital transcript expression analysis - Accurate quantitation of expression levels over 5 orders of magnitude of transcript abundance.
• Detect rare transcripts and transcript variants, such as single nucleotide mutations (SNP).

Our Comprehensive Service

This service is comprehensive - from sample to data, providing advanced technology and years of experience in efficient discovery and profiling of microRNAs.

Our “Total RNA to Data” comprehensive service includes: sample QC, sample preparation (small RNA selection,  library preparation, single molecule clonal amplification), high-throughput sequencing, bioinformatics analysis, and customer data report.

Sample Multiplexing - We have the ability to index your small RNA samples and combine several samples in each sequencing lane.  Sample indexes added during the sample prep act as “barcodes” so reads can be attributed to a specific sample during data analysis.  For an unknown sample, it is useful to start with standard sequencing.  When there are available data, you can estimate if further sequencing can be done with indexing/barcoding to take the advantage of the full capacity of the sequencing technologies.

Optional target-specific selection service is available to enrich your sample(s) for more focused sequencing to achieve deeper coverage.

Down-stream profiling of identified (and other customer defined target microRNAs) is also available by custom microarray analysis service.

Data Analysis / Results

• Standard Solexa pipeline data analysis is included.
• Additional LC Sciences software processing is included.
• Data is filtered and reduced to mappable reads.

For each sample analyzed, the customer receives:

• Either FASTA-A or FASTA-Q files with base-calling quality scores.
• A list of detected sequences.
• Copy number per sequence.

Additional in-depth analysis is available:

• Map to reference sequence - Sequence data can be aligned to publicly available microRNA sequence databases.
• Differential expression analysis for multiple sample experiments - t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.

Sitemap • Privacy • Terms of Use • Disclaimer

Copyright © 2009, LC Sciences