microRNA Detection Microarray Service

Qin et al. (2010) MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells. Proc Natl Acad Sci U S A 107(7), 3240-244. [abstract]

Greene et al. (2010) A putative role for microRNA-205 in mammary epithelial cell progenitors. J Cell Sci [Epub ahead of print] [abstract]

Pogribnyet al. (2010) Alterations of microRNAs and their targets are associated with acquired resistance of MCF-7 breast cancer cells to cisplatin. Int J Cancer [Epub ahead of print] [abstract]

Yuan et al. (2010) MicroRNA expression changes in the hippocampi of rats subjected to global ischemia. J Clin Neurosci [Epub ahead of print] [abstract]

Nunez-Iglesiaset al. (2010) Joint genome-wide profiling of miRNA and mRNA expression in Alzheimer's disease cortex reveals altered miRNA regulation. PLoS One 5(2), e8898. [abstract]

Caruso et al. (2010) Dynamic Changes in Lung MicroRNA Profiles During the Development of Pulmonary Hypertension Due to Chronic Hypoxia and Monocrotaline. Arterioscler Thromb Vasc Biol [Epub ahead of print] [abstract]

Zeiger U, Khurana TS. (2010) Distinctive Patterns of MicroRNA Expression in Extraocular Muscles. Physiol Genomics [Epub ahead of print] [abstract]

LC Sciences provides a genome-wide microRNA (miRNA) expression profiling service using µParaflo^®technology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs. We have standard arrays for mature miRNA of all species available in the latest version of the Sanger miRBase database (Release 14.0). Our service is comprehensive and includes small RNA enrichment from your total RNA sample, single or dual color labeling, hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we send you the analyzed data, representative and original images of the array, and raw data. We offer additional products and services including arrays containing miRNA*, walk/tiling arrays, custom arrays (first 100 probes free of charge), and in-depth analysis for multiple data sets. The in-depth data analysis may include multi-array normalization, t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.

Special Announcement - Plant MicroRNA Database Content - Now Available!

October 8th, 2009 - The plant miRNA database (PMRD) integrates available plant miRNA data deposited in public databases, gleaned from the recent literature, and data generated by the database organizers. This database contains sequence information, secondary structure, target genes, expression profiles and a genome browser.

In total, there are 8433 miRNAs collected from 121 plant species in PMRD, including model plants and major crops such as Arabidopsis, rice, wheat, soybean, maize, sorghum, barley, etc in this first edition of the database. (read more...)

This plant probe content is immediately available on all of our standard or custom microRNA microarrays.

Featuring µParaflo® Biochip Technology - A high performance microfluidic custom microarray platform.

100% miRBASE VERSION 14.0 CONTENT
COMPLETE CONTENT FLEXIBILITY (All Species)
OPTIMIZED RNA HYBRIDIZATION PROBES
EXPERIENCED SERVICE PROVIDER HANDLING VARIOUS RESEARCH & CLINICAL SAMPLE TYPES
(Including FFPE and Blood/Plasma Samples)
COST EFFECTIVE 1-STOP SOLUTION

Comprehensive Service Available	√
Microarray Platform	µParaflo^®Microfluidic Biochip Technology
Dual Color Capable	√
Starting Sample Material	Total RNA, miRNA, Blood, Cell Pellet, Tissue, FFPE
Sample Requirement	5 µg Total RNA - If you are limited in sample amount, please give us a call. We can accommodate smaller sample sizes.
Probe Content	miRBase 14.0 (September 2009)
Species Covered	All species for which data exists (in Sanger miRBase)
Probes T_m Equalized	√
Add Custom Sequences	√
Detection Limit	< 10 attomole
Dynamic Range	>3.5 logs
Full Data Analysis Included	√
Data Delivery Time	3-4 Weeks
Number of Customer Publications to Date	>100

Latest Product News

Sept 15, 2009 - With the addition of 1580 new mature miR and miR* sequences, the microRNA Sequence Database (miRBase) announced a significant update to the sequence database which has now topped 10,000 entries. (read more...)

March 9, 2009 - With the addition of 1004 new mature miRNA sequences, the Sanger microRNA Sequence Database (miRBase) announced a significant update to the miRBase sequence database. This latest release is miRBase version 13.0 and features a total of 9169 mature miRNA sequences. (read more...)

Sept 1, 2008 - The Sanger Institute announces the largest single update in the history of miRBase with the of 2062 new mature miRNA sequences. In addition to sequence updates and additions to species included in previous miRBase versions, mature miRNA sequences from many new species are included for the first time. (read more...)

May 6, 2008 – LC Sciences announced today that it has entered into a strategic marketing alliance with DNAVision to increase accessibility to its custom microRNA profiling service and enhance both its customer and technical service to researchers in Europe. (read more...)

April, 2008 - The Sanger miRBase sequence database was updated to release version 11.0. The number of verified microRNAs has been increased from 5395 to 6396 hairpin precursor miRNAs expressing 6211 mature miRNA products. This latest microRNA sequence content is currently available on all our standard and custom microRNA microarrays. (read more...)

February, 2008 – LC Sciences tops 30th customer publication. LC Sciences announced today the publication of the 30th peer-reviewed study by one of its customers using the company’s microarray service for analyzing microRNA expression profiles and for discovery of novel small RNAs. These studies, by leading researchers in the field, contribute to a fast growing body of knowledge defining this recently discovered class of regulatory RNAs. (read more...)

December, 2007 - The Sanger miRBase sequence database was updated to release version 10.1. This marks the fourth update this year. The number of verified microRNAs has been increased from 5071 to 5395 hairpin precursor miRNAs expressing 5234 mature miRNA products. This latest microRNA sequence content is currently available on all our standard and custom microRNA microarrays. (read more...)

Overview of the Service

Microfluidics Microarray Platform

The µParaflo® technology integrates high throughput parallel synthesis, microfluidics, and digital photolithography to create custom arrays of DNA, RNA, or peptides. Our advanced microarray platform delivers more reliable results than are possible with traditional spotted arrays.

Optimized RNA Hybridization Probes

LC Sciences’ Optimized RNA Hybridization Probes are designed with normalized Tms to ensure uniform hybridization affinity to their microRNA targets under high stringency hybridization conditions enhancing both the sensitivity and specificity of the probes.

The Most Current Content

LC Sciences offers the only microRNA microarray currently available with version 14.0 probe content. Not 90% or 95% coverage, but 100% of experimentally verified microRNA sequences are represented on all of our arrays.

Complete Content Flexibility

All of LC Sciences’ microarrays are made to order and synthesized to each customer’s particular specifications. Validate predictions, add custom controls, or create totally custom tiling arrays for discovery of novel small-RNAs. The choice is up to you!

No Limitation on Use of Data

Unlike with proprietary probe content, we hold no claims on your data. You have full access and control over the results generated from your experiments.

The Standard for Array Data Quality

The µParaflo^® technology enables on-chip synthesis ensuring high probe quality and tight process control. The data obtained by these microarrays is highly reliable and reproducible as validated by qPCR and Northern Blot.

In Depth Data Analysis

LC Sciences’ in-depth data analysis includes all t-Tests, ANOVA and heat maps to get you the information you need. Complex multi-array experiments are no problem for our experienced technical team. We can easily compare samples from different chips or even new experimental data to data from previous experiments.

The Most Customer Publications

LC Sciences' customers have published over 100 papers to date; have confirmed that microRNAs play an important role in a wide array of biological functions.

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