Sample Preparation
Sample Amount Required
- Please transfer your sample to a 1.5 ml microcentrifuge tube for shipment (0.5 ml or smaller PCR tubes can crack when frozen) unless otherwise specified.
- Total RNA - 10 µg / assay
- For customers with limited amounts of total RNA - We can apply the Affymetrix two-cycle amplification kit if you can achieve at least 100ng of total RNA.
- Cells - 2-5 x 106
- Tissue - 200 mg
- Blood - 3 PAXgene Blood RNA tubes with a total of approximately 6 ml og peripheral blood.
RNA Extraction
- We strongly recommend that total RNA be isolated by Qiagen RNeasy kit for consistent quality.
- Please notify LC Sciences if the total RNA is isolated by commercial kits other than Qiagen.
- Elute total RNA with RNase-free water as indicated in the protocol.
- Transfer your sample to a 1.5ml microcentrifuge tube for shipment (smaller tubes can crack when frozen).
Quality Control of RNA Sample
- A quality control check of your total RNA sample is included in our comprehensive service.
- Additionally, you can check the UV spectrum of your sample with a spectrophotometer. The 260nm/230nm ratio should be greater than 1.0 and the 260nm/280nm ratio should be above 1.8.
- Note - We will notify you if samples fail our quality control analysis before proceeding with your microarray experiment.
Placing Your Order
Determine Array Content and Select Services
- See the list of
available arrays and select the species or species group you are interested in.
- Select any additional services.
- Contact us and request a quote for your experiment.
Complete a Sample Submission Form
- Complete a
sample submission form prior to sending your sample to us.
- The form can be emailed to us at:
sales@lcsciences.com beforehand or sent in the package along with your sample. Note - If you send the form in the package with samples please place it in a separate waterproof bag.
Packaging and Shipping
- Please transfer your sample to a 1.5ml microcentrifuge tube for shipment (0.5 ml or smaller PCR tubes can crack when frozen) unless otherwise specified for your sample type. Be sure the tube labels match those listed on your sample submission form.
- If you want to seal the tubes or hold them in a rack, please don't use tape (it will crack when frozen), use parafilm.
- Pack the sample with dry ice in a thermal insulated shipping box.
- Ship the package by overnight carrier for delivery the next day.
- Note - Do not ship samples on Friday as they will sit over the week-end and deteriorate. Wait until the following Monday and ship for Tuesday delivery.
Mail the package to:
Attn: Sample Receiving
LC Sciences
2575 West Bellfort St., Suite 270
Houston, TX 77054
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