Human mesenchymal stem/stromal cells (hMSCs) provide support for cancer progression, partly through their secretome that includes extracellular vesicles (EVs). Through small RNA sequencing from EVs of MSCs, researchers at University of Mississippi Medical Center now report the characterization of novel small RNA, named n-miR-G665, which exhibits typical properties of miRNAs.

The n-miR-G665 sequence is conserved and expressed in most cell types. Knockdown studies using anti-agomirs and shRNA studies demonstrated that n-miR-G665 plays an important role in cell proliferation. Functional assays to reveal the targets of n-miR-G665 showed that polycomb protein Suz12 is regulated by n-miR-G665, which in turn regulates the expression of n-miR-G665 through feedback loop mechanism.

These data shed light on a previously unknown novel feedback regulatory mechanism for controlling Suz12 expression regulated by previously not described miRNA. This may highlight a new therapeutic approach to control the polycomb repressor complex 2 activity in cancers.


P. Penfornis, J. D. Fernandes, R. R. Pochampally (2017) Polycomb group protein Suz12 is regulated by a novel miRNA-like small RNA Scientific Reports doi: 10.1038/s41598-018-19989-5 [article]

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