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microRNA Study in Non-Model Species

LC Sciences’ custom microRNA microarray service is based on the flexible µParaflo® microfluidic chip technology which enables us to produce custom synthesized microarrays when ordered. (vs. an off-the-shelf spotted array)  Therefore, researchers are not limited to the species (and sequences) listed in miRBase.  They can add any sequence of their design to the standard miRBase probe content.

Customizable features include – sequence design, varying chain lengths, chip layout, synthesis chemistry, and more.   Each µParaflo® microfluidic chip has room for thousands of sequences of your design.  Add sequences for various applications:

  • Screen for new microRNAs by adding predicted mature microRNA sequences or perform sequence tiling along certain sequences sections.
  • Combine microRNA sequences of different species to identify cross-species conservations or host-parasite interaction.
  • Add controls of customer’s choice for the detection of customer-added spiking RNA sequences and use as customer-selected internal controls.
  • Add probes for the detection of siRNAs and/or other small non-coding RNAs.
  1. Liu G, Fang Y, Zhang H, Li Y, Li X, Yu J, Wang X. (2010) Computational identification and microarray-based validation of microRNAs in Oryctolagus cuniculus.  Mol Biol Rep [Epub ahead of print]  [abstract]
  2. Legeai F, Rizk G, Walsh T, Edwards O, Gordon K, Lavenier D, Leterme N, Mereau A, Nicolas J, Tagu D, Jaubert-Possamai S. (2010) Bioinformatic prediction, deep sequencing of microRNAs and expression analysis during phenotypic plasticity in the pea aphid, Acyrthosiphon pisum. BMC Genomics 11(1), 281.  [abstract]
  3. Gundersen-Rindal DE, Pedroni MJ. (2010) Larval stage Lymantria dispar microRNAs differentially expressed in response to parasitization by Glyptapanteles flavicoxis parasitoid. Arch Virol (5), 783-87.  [abstract]
  4. Dongdong L, Yusheng Zheng, Li Wan, Xiaoming Zhu and Zhekui Wang. (2009) Differentially expressed microRNAs during solid endosperm development in coconut (Cocos nucifera L.). Scientia Horticulturae 122(4), 666-69.  [abstract]
  5. Sehm T, Sachse C, Frenzel C, Echeverri K. (2009) miR-196 is an essential early-stage regulator of tail regeneration, upstream of key spinal cord patterning events. Dev Biol 334(2), 468-80.  [abstract]