LC Sciences News

Visit LC Sciences at Ft Detrick

admin — Mon, 07 May 2012 19:04:06 +0000

Visit LC Sciences at Booth #209 – May 9-10, 2012 – Frederick, MD – NCI-Frederick and Ft. Detrick Spring Research Festival

Visit LC Sciences at Ft Detrick is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

Study of Plant microRNAs by Small RNA Sequencing

admin — Fri, 27 Apr 2012 13:19:08 +0000

Researchers at Zhejiang University, China have not only identified 64 cucumber miRNAs, which belongs to 29 known and two novel miRNA families, but also discovered that some of the miRNAs are differentially expressed in a tissue-dependent manner¹. For the first time, we detected 21 sliced targets, which reveal interaction between miRNA and target in cucumber by using the recently developed degradome sequencing approach. This report will offer a foundation for future studies of the miRNA-mediated regulatory networks in cucumber.

The first genome-wide study for the identification of larch miRNAs by high-throughput sequencing strategy and the expression profiles of miRNAs and their targets in larch SE². Although 83 conserved miRNAs were identified and 16 novel miRNAs plus 14 plausible miRNA candidates were discovered, there are still a great number of miRNAs that remain to be identified. This work contributes to the expression profiles of eleven miRNA families and their targets during the eight stages of larch SE. This work represents a significant step towards illuminating the functions of miRNAs during gymnosperm embryogenesis. The acquisition of precursor sequences lays the foundation for future functional studies of selected miRNAs during embryogenesis of larch, which is currently underway.

Recently, substantial research has been directed at improving the N-use efficiency of plants. A study led by researchers at the China Agricultural University has focused on maize, a valuable graminaceous monocot that provides feed, fodder, and biofuel³. By deep sequencing four small RNA libraries and one degradome from maize seedlings exposed to N deficiency, they were able to add 47 new miRNA families to the previously published 26 families in maize. In addition, we identified three, five, and one new member for the families of miR171, miR169, and miR398, respectively.

Mao W, Li Z, Xia X, Li Y, Yu J. (2012) A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber. PLoS One 7(3), e33040. [article]
Zhang J, Zhang S, Han S, Wu T, Li X, Li W, Qi L. (2012) Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis. Planta [Epub ahead of print]. [abstract]
Zhao M, Tai H, Sun S, Zhang F, Xu Y, et al. (2012) Cloning and Characterization of Maize miRNAs Involved in Responses to Nitrogen Deficiency. PLoS ONE 7(1), e29669. [article]

Study of Plant microRNAs by Small RNA Sequencing is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

Small RNA Sequencing Enables Study of Virus microRNA

admin — Thu, 26 Apr 2012 21:21:02 +0000

Yao Y, Smith LP, Petherbridge L, Watson M, Nair V. (2012) Novel microRNAs encoded by duck enteritis virus. J Gen Virology [Epub ahead of print]. [abstract]
Hancock MH, Tirabassi RS, Nelson JA. (2012) Rhesus cytomegalovirus encodes seventeen microRNAs that are differentially expressed in vitro and in vivo. Virology 425(2), 133-42. [abstract]
Ma M, Huang Y, Gong Z, Zhuang L, Li C, et al. (2011) Discovery of DNA Viruses in Wild-Caught Mosquitoes Using Small RNA High throughput Sequencing. PLoS ONE 6(9), e24758. [article]
Meyer C, Grey F, Kreklywich CN, Andoh TF, Tirabassi RS, Orloff SL, Streblow DN. (2010) Cytomegalovirus MicroRNA Expression Is Tissue Specific and Is Associated with Persistence. J Virol 85(1), 378-89. [abstract]

Small RNA Sequencing Enables Study of Virus microRNA is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

Prostacyclin-microRNA regulation in obesity and atherosclerosis

admin — Thu, 26 Apr 2012 20:36:58 +0000

Researchers at the University of Houston used LC Sciences’ miRNA microarray service and discovered that prostacyclin (PGI₂), a key vascular protector, is involved in regulating cellular microRNA (miRNA) expression through its receptors in a mouse adipose tissue-derived primary culture cell line expressing a novel hybrid enzyme gene.

Their array data suggest that PGI₂ regulates miRNA expression that inhibits insulin-mediated lipid deposition in cells. This is relevant because peripheral lipid deposition (obesity) and uncontrolled subendothelial lipid deposition in smooth muscle cells (atherosclerosis) could be directly related to impaired PGI₂ production.

This indicates that PGI₂-miRNA regulation could exist in broad pathophysiological processes involving PGI₂ (i.e., apoptosis, vascular inflammation, cancer, embryo implantation, and obesity).

Mohite A, Chillar A, So SP, Cervantes V, Ruan KH. (2011) Novel mechanism of the vascular protector prostacyclin: regulating microRNA expression. Biochemistry 50(10), 1691-99. [abstract]

Prostacyclin-microRNA regulation in obesity and atherosclerosis is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

Visit LC Sciences at Drug Discovery Chemistry

admin — Mon, 16 Apr 2012 18:54:38 +0000

LC Sciences will be exhibiting this week in San Diego for the Drug Discovery Chemistry Conference.

Booth #19

We will also be presenting a poster: Phosphopeptide Array Platform for Cellular Signaling Network Protein Profiling in Breast Cancer Cells

Peptide Microarray technology for studying protein-protein interactions.

Visit LC Sciences at Drug Discovery Chemistry is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

OligoMix for Drug Discovery & Development

admin — Mon, 16 Apr 2012 18:02:26 +0000

Microarray-Synthesized Oligos for Targeted Sequencing

Thu, 04/12/2012 – 3:28pm

Chris Hebel, Vice President of Business Development; LC Sciences, Houston

Though next-generation DNA sequencing (NGS) provides very high levels of coverage even on complex genomes, it is still advantageous to reduce the complexity of samples and sequence smaller targeted regions—in particular, when sample numbers are very high and the goal is detection of less prevalent mutations. Several academic and commercial groups have developed a variety of capture methods for enriching or selectively amplifying subsets of the genome for targeted sequencing. (read more…)

OligoMix for Drug Discovery & Development is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

Recent Online Customer Publications

admin — Tue, 10 Apr 2012 19:28:04 +0000

microRNA Discovery Sequencing Service

Yao Y, Smith LP, Petherbridge L, Watson M, Nair V. (2012) Novel microRNAs encoded by duck enteritis virus. J Gen Virol [Epub ahead of print]. [abstract]

Seq-Array Service

Meng F, Hackenberg M, Li Z, Yan J, Chen T. (2012) Discovery of Novel MicroRNAs in Rat Kidney Using Next Generation Sequencing and Microarray Validation. PLoS One 7(3), e34394. [article]

microRNA Profiling Microarray Service

Ma YJ, Yang J, Fan XL, Zhao HB, Hu W, Li ZP, Yu GC, Ding XR, Wang JZ, Bo XC, Zheng XF, Zhou Z, Wang SQ. (2012) Cellular MicroRNA let-7c Inhibits M1 Protein Expression of the H1N1 Influenza A Virus in Infected Human Lung Epithelial Cells. J Cell Mol Med [Epub ahead of print]. [abstract]
Kovalchuk A, Lowings M, Rodriguez-Juarez R, Muhammad A, Ilnytskyy S, Kolb B, Kovalchuk O. (2012) Epigenetic bystander-like effects of stroke in somatic organs. Aging [Epub ahead of print] [abstract]
Yang L, Cheng P, Chen C, He HB, Xie GQ, De Zhou H, Xie H, Wu XP, Luo XH. (2012) miR-93/Sp7 function loop mediates osteoblast mineralization. J Bone Miner Res [Epub ahead of print]. [abstract]
Mondou E, Dufort I, Gohin M, Fournier E, Sirard MA. (2012) Analysis of microRNAs and their precursors in bovine early embryonic development. Mol Human Reprod [Epub ahead of print]. [abstract]

Recent Online Customer Publications is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

New Free Sample Shipping Kit Available

admin — Tue, 27 Mar 2012 15:25:09 +0000

We want to take the hassle out of shipping your sample(s) to us. We’re now offering a free service to researchers who are planning to send samples to LC Sciences for analysis. Click the link on our website and enter your contact information and we’ll send you a shipping kit that includes everything you need to send your sample(s) to LC Sciences. You’ll just need to add your sample(s) and some dry ice to the shipping box. There is no cost for us to send you the shipping kit. The cost for shipping the sample(s) to us will be billed to our shipping account and we’ll add it to your order invoice.

(Currently available for US shipping only)

New Free Sample Shipping Kit Available is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

Visit LC Sciences at AACR 2012

admin — Mon, 26 Mar 2012 19:30:45 +0000

Visit LC Sciences at this year’s meeting of the American Association of Cancer Research (AACR – 2012). We are located in booth #4402.

microRNA had been definitively link to cancer since a trio of papers were published in Nature back in 2005. Since then there have been thousands of studies (including over 100 published studies by our customers) detailing the role of microRNA in cancer progression, metastasis, chemotherapy resistance, as a tumor-suppressor, a potential drug target or diagnostic biomarker, and more.

LC Sciences provides microRNA discovery sequencing service based on the Illumina Genome Analyzer, the clear front-runner of the latest next-gen sequencing technologies. If you’re working with a poorly characterized species, want to discover previously unknown miRNAs, or are interested in true isoform abundance, then microRNA analysis by sequencing makes sense.

LC Sciences provides a microRNA microarray service is based on our own µParaflo® custom microfluidic microarray platform. Our arrays always contain 100% of current miRBase content plus your custom sequences, and have set the standard for microarray data quality. Microarrays have been a robust method for expression profiling for decades and is an economical choice for profiling large numbers of samples from well-characterized speices.

Please stop by booth #4402 and we would be happy to discuss how our microRNA discovery and profiling services can benefit your cancer research.

Visit LC Sciences at AACR 2012 is a post from: LC Sciences Blog. More information about LC Sciences can be found here.

LC Sciences Supports the Texas Genetics Society

admin — Mon, 26 Mar 2012 19:05:16 +0000

The annual meeting of the Texas Genetics Society was held last week in San Antonio and LC Sciences is proud to have been a sponsor at the meeting. The Texas Genetics Society was established in 1974 as a nonprofit association whose purpose is to foster the development of all aspects of genetics, to promote the exchange of research results and the teaching of genetics, and to provide a forum for discussion of matters of interest to all geneticists. This year’s meeting included interesting presentations by researchers across the state, including: UT Southwestern, UT San Antonio, UTHSCSA, TAMU College Station, TAMHSC, UTHSCH, UT MDACC, University of Houston, SBIR San Antonio, Texas Tech University and others. Additionally there were many scientific posters presented by undergrads, graduate students, and postdocs.

LC Sciences Supports the Texas Genetics Society is a post from: LC Sciences Blog. More information about LC Sciences can be found here.