Epitope Mapping by Printed Peptide Libraries
Frank Breitling1 , Christopher Schirwitz2, Thomas Felgenhauer2, Ines Block2, Volker Stadler2 and Ralf Bischoff2
||Karlsruhe Institute of Technology, Helmholtzplatz 1, 76344 Eggenstein-Leopoldshafen, Germany
||AG Chipbasierte Peptidbibliotheken, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
Affordable high-density peptide arrays are needed to routinely define the exact binding sites of antibodies. In terms of prize and density peptide arrays currently lag far behind oligonucleotide arrays that are available in densities exceeding 50.000 oligonucleotides per cm2. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with the lithographic methods, which adds up to an excessive number of coupling cycles. The combinatorial synthesis of peptide arrays based on electrically charged solid amino acid particles circumvents this problem. A colour laser printer or a microchip consecutively address the different charged particles to a solid support, where a complete layer of solid amino acid particles is melted at once. This releases hitherto immobilized amino acids to couple all 20 different amino acids to the support in one single coupling reaction.
LC Sciences offers a comprehensive epitope mapping service for high throughput, high-resolution identification of epitopes and other protein-protein interactions.
Through the use of overlapping peptides as epitopes on a custom synthesized addressable peptide microarray (PepArray™), we can systematically screen thousands of sequences in a single experiment. A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events.
This combination of high-throughput capacity with quantitative measurement enables us to quickly and efficiently identify high affinity and high specificity target binding compounds.