The miRBase sequence database was updated to version 19 on August 1st 2012. Release 19 of the database contains 21,264 entries representing hairpin precursor miRNAs, expressing 25,141 mature miRNA products, in 193 species. As compared to miRBase version 18 a total of 3,171 new hairpin sequences and 3,625 novel mature products have been added. Read more about this update. µParaflo® Technology enables on demand synthesis of microarrays. That means this new probe content is immediately available on all
Recent Work & Current Methods in Plant microRNA Research
We want to take the hassle out of shipping your sample(s) to us. We’re now offering a free service to researchers who are planning to send samples to LC Sciences for analysis. Click the link on our website and enter your contact information and we’ll send you a shipping kit that includes everything you need to send your sample(s) to LC Sciences. You’ll just need to add your sample(s) and some dry ice to the shipping box. There is no
See the transcriptomics services offered by LC Sciences.
A comprehensive service package From sample to data, our comprehensive profiling service includes: sample QC and library preparation, high-throughput sequencing, and all bioinformatics analysis. Differential expression results are delivered in a concise report format. Many advantages over gene expression with microarrays Expression profiling is truly genome-wide. There is no need for probes based on prior sequence information. You can achieve digital transcript expression
microRNA / Small RNA Discovery Sequencing Service Next-gen sequencing is a new method and powerful tool used to identify and quantitatively decode the entire population of small RNAs in your total RNA sample. LC Sciences provides a comprehensive discovery sequencing service utilizing Illumina’s high-throughput sequencing technology which enables comprehensive, highly sensitive and specific discovery and profiling of all forms of small RNAs in your sample. Whole Transcriptome Sequencing Service RNA-Seq
InDepth Data Analysis Report Includes: Map to Reference Sequence Includes custom construction of reference database(s) for mapping - miRBase, genome, etc Sequence data can be aligned to any publicly available small RNA databases for annotation of known small RNA Unknown small RNAs can be aligned with reference genomes Additional Bioinformatics Analysis Classification of all mapped reads Length distribution of mapped sequences Annotation documentation of mapped sequences Alignment of sequence variants such as isomirs Genomic and chromosomal location of sequence clusters Prediction of new possible miRs Detailed explanation of miRNA analysis results
Please nte that the sample submission form for microRNA Microarray Service has been updated due to the update of miRBase to version 15. If you have a previous version of the form saved locally, please replace with this new form. Click the link below or visit www.lcsciences.com to download the form. Thank You
Transcriptomics, or genome-wide expression profiling, aims to catalogue the complete set of RNA transcripts produced by the genome, including mRNAs, non-coding RNAs and small RNAs. Transcriptomics provides new biological insight and can be used to determine the structure of genes, their splicing patterns and other post transcriptional modifications, to detect rare and novel transcripts, and to quantify the changing expression levels of each transcript during development and under different disease conditions. LC Sciences now provides a one-stop solution (i.e. from sample
30K Custom Array Design - LC Sciences has completed development of a new microfluidic chip design containing 30K features. We are currently accepting early access orders on a case-by-case basis for custom arrays built on this design for both microRNA profiling and epitope mapping applications. All of our array designs are based on the patented µParaflo® custom microarray platform.
Seq-ArraySM offers a customized solution to high-throughput genome-wide miRNA discovery and profiling, especially in species with limited or no miRNA sequence information available. This unique combination of the latest deep sequencing technology, advanced bioinformatics, and our innovative µParaflo® custom microarray platform leverages all these technologies to form a comprehensive service package tailored to your specific research needs. Seq-ArraySM provides an efficient pathway from an initial broad miRNA search
LC Sciences' microRNA microarrays cover all species for which sequence data are available in the miRBase Sequence Database and the Plant MicroRNA Database. Other suppliers are not covering miRs from the Plant MicroRNA database and their arrays covering the miRBase are somewhat out of date. Since miRBase is being continually updated, a flexible microarray platform is necessary to keep current with the latest miRBase version (14.0 released in September
LC Sciences now offers a comprehensive epitope mapping service for high throughput, high-resolution identification of epitopes and other protein-protein interactions. Through the use of overlapping peptides as epitopes on a custom synthesized addressable peptide microarray (PepArray™), we can systematically screen thousands of sequences in a single experiment. A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events. This combination of high-throughput capacity with quantitative
Why synthesize thousands of oligonucleotides individually? Why perform thousands of individual reactions while handling thousands of tubes? When you can Synthesize one OligoMix®. Perform thousands of parallel (multiplex) reactions in a single tube. Save time and money. Be kind to our environment. For instance; for a small genome of 5Mbp, 250,000 oligos of 40mers are required. To synthesize thousands of oligo sequences individually would be expensive, equipment intensive, impractical. Making thousands of oligonucleotides consumes a huge amount of
LC Sciences provides a unique new tool, OligoMix® enabling low cost, multiplex custom oligonucleotides for various applications serving diverse needs in genomics, proteomics, protein/antibody engineering, synthetic biology, drug discovery, and emerging new fields of science such as next-generation sequencing. OligoMix® is a MIXTURE of a large number of DESIGNED synthetic DNA oligonucleotide sequences delivered in a single microtube. OligoMix® is a product developed in response
LC Sciences now has standard arrays available for all species listed in the newly created Plant MicroRNA Database (PMRD). The plant miRNA database (PMRD) integrates available plant miRNA data deposited in public databases, gleaned from the recent literature, and data generated by the database organizers. This database contains sequence information, secondary structure, target genes, expression profiles and a genome browser. In total, there are 8433 miRNAs collected from 121 plant species in PMRD, including model plants and major crops such as Arabidopsis,
The current probe content comes from version 14.0 of the miRBase sequence database which was updated on September 15th, 2009. (See the 14.0 release summary) (See a comparison to previous versions) Version 14.0 of the miRBase 14.0 release summary Comparison to previous versions We can synthesize microRNA arrays for all species that are listed in the miRBase Sequence Database. We have listed a subset of our most popular species below.