New Customer Publications

Metastasis is one of the hallmarks of cancer malignancy that usually causes more detrimental effects than a primary tumor. Many microRNAs have been reported to be involved in the process of tumor metastasis. Now, researchers from the Chinese Academy of Medical Sciences and Peking Union Medical College studied microRNAs in Hep11 and Hep12 cells derived from primary and recurrence (intrahepatic metastatic) sites of hepatocellular carcinoma (HCC), respectively. They found that Hep12 exhibited a higher invasive and migratory potential than Hep11. There was

MicroRNAs are now recognized as key post-transcriptional regulators in animal ontogenesis and phenotypic diversity. Eupolyphaga sinensis Walker (Blattaria) is a sexually dimorphic insect, which is also an important source of material used in traditional Chinese medicine. The male E. sinensis have shorter lifecycles and go through fewer instars than the female. Furthermore, the males have forewings, while the females are totally wingless. Researchers at the Nanjing Normal University, China used the Illumina/Solexa deep sequencing technology to sequence small RNA

The differentiation of human peripheral blood monocytes into macrophages can be reproduced ex vivo by culturing the cells in the presence of Colony-Stimulating Factor 1 (CSF1). Using microarray profiling to explore the role of microRNAs (miRNAs), researchers from the French Institute of Health and Medical Research have identified a dramatic decrease in the expression of the hematopoietic specific miR-142-3p. Up- and down-regulation of this miRNA in primary human monocytes altered CSF1-induced differentiation of

OligoMix CE Meacham. (2012) In vivo pool-based shRNA screens to identify modulators of disease progression in hematopoietic malignancies. MIT Thesis [abstract] microRNA Sequencing Wu W, Ren Q, Li C, Wang Y, Sang M, Zhang Y, Li B. (2013) Characterization and Comparative Profiling of MicroRNAs in a Sexual Dimorphism Insect, Eupolyphaga sinensis Walker. PLoS ONE 8(4), [abstract] Ma J, Yu S, Wang F, Bai L, Xiao J, Jiang Y, Chen L, Wang J, Jiang A, Li M. (2013) MicroRNA Transcriptomes

MicroRNAs (miRNAs) have been implicated in the regulation of milk protein synthesis and development of the mammary gland (MG). However, the specific functions of miRNAs in these regulations are not clear. Therefore, the elucidation of miRNA expression profiles in the MG is an important step towards understanding the mechanisms of lactogenesis. Two miRNA libraries were constructed from MG tissues taken from a lactating and a non-lactating Holstein dairy cow, respectively, and the

Derivation of human embryonic stem cells (hESCs) provides a powerful tool to investigate the molecular controls in human embryonic neurogenesis as well as an unlimited source to generate the diversity of human neuronal cell types in the developing CNS for repair. Realizing the developmental and therapeutic potential of hESCs has been hindered by conventional multi-lineage differentiation of pluripotent cells, which is uncontrollable, inefficient, highly variable, difficult to reproduce and scale-up. We recently

Sequencing Liu N, Yang J, Guo S, Xu Y, Zhang M. (2013) Genome-Wide Identification and Comparative Analysis of Conserved and Novel MicroRNAs in Grafted Watermelon by High-Throughput Sequencing. PloS one 8(2). [article] Microarray Wang W, Cheng B, Miao L, Mei Y, Wu M. (2013) Mutant p53-R273H gains new function in sustained activation of EGFR signaling via suppressing miR-27a expression. Cell Death & Disease 4(4). [abstract] Tzarfati R, Ben-Dor S, Sela I, Goldschmidt EE. (2013) Graft-induced Changes in MicroRNA Expression Patterns

Gall midges encompass a large group of insects that are of economic importance and also possess fascinating biological traits. The gall midge Mayetiola destructor, commonly known as the Hessian fly, is a destructive pest of wheat and model organism for studying gall midge biology and insect – host plant interactions. In this study, a team led by researchers in the Department of Entomology at Kansas State University systematically analyzed miRNAs from the Hessian fly by

Papillary thyroid carcinoma (PTC) is the most common malignant thyroid tumor. Despite its well-differentiated characteristics, papillary carcinoma may be overtly or minimally invasive. In fact, these tumors may spread easily to other organs. The life expectancy of patients with this cancer is related to their age. The global incidence shows an increase of 4% annually for this kind of tumor, in the eighth place of tumor incidence in females, seriously threatening human health and life. MicroRNA (miRNA) microarray analysis has consistently

Yang Z, Wu L, Zhu X, Xu J, Jin R, Li G, Wu F. (2013) MiR-29a modulates the angiogenic properties of human endothelial cells. Biochem Biophys  Res Comm [Epub ahead of print]. [abstract] Fu HL, Wu DP, Wang XF, Wang JG, Jiao F, Song LL, Xie H, Wen XY, Shan HS, Du YX. (2013) Altered miRNA Expression is Associated with Differentiation, Invasion, and Metastasis of Esophageal Squamous Cell Carcinoma (ESCC) in Patients from Huaian, China. Cell Biochem Biophys [Epub

The anti-tumor effect of vitamin D has been well recognized but its translational application is hindered by side effects induced by supra-physiological concentration of vitamin D required for cancer treatment. Thus, exploring the vitamin D tumor suppressive functional mechanism can facilitate improvement of its clinical application. A team of researchers from the University of Rochester and the University of Tainan, Taiwan used microarrays to screen miRNA profiles in response to vitamin D and found that

Kumar P, Luo Y, Tudela C, Alexander JM, Mendelson CR. (2013) The c-Myc Regulated microRNA (miR)-17~92 and miR-106a~363 Clusters Target CYP19A1 and hGCM1 to Inhibit Human Trophoblast Differentiation. Mol Cell Biol [Epub ahead of print]. [abstract] Zhao Y, Bhattacharjee S, Jones BM, Dua P, Alexandrov PN, Hill JM, Lukiw WJ. (2013) Regulation of TREM2 expression by an NF-kB-sensitive miRNA-34a. NeuroReport [Epub ahead of print]. [abstract] Thapa S, V Chandrasekaran V. (2013) The Role of MicroRNA in BMP-7 induced

The gestational state is a period of particular vulnerability to diseases that affect maternal and fetal health. Stress during gestation may represent a powerful influence on maternal mental health and offspring brain plasticity and development. In a fascinating new study,  a team led by researchers at the University of Lethbridge, Canada show that the fetal transcriptome, through microRNA (miRNA) regulation, responds to prenatal stress in association with epigenetic signatures of psychiatric and

MicroRNAs (miRNAs) as small non-coding RNAs play important roles in many biological processes such as development, cell signaling and immune response. Studies also suggest that miRNAs are important in host-virus interactions where host limits virus infection by differentially expressing miRNAs that target essential viral genes. Researchers at the University of Queensland used a combination of deep sequencing an microarray to study how microRNAs are involved in the immune response in insects. Spodoptera frugiperda (army worm) In the first study, identified conserved and

Small RNA Sequencing Hébert SS, Wang WX, Zhu Q, Nelson PT. (2013) A Study of Small RNAs from Cerebral Neocortex of Pathology-Verified Alzheimer’s Disease, Dementia with Lewy Bodies, Hippocampal Sclerosis, Frontotemporal Lobar Dementia, and Non-Demented Human Controls. J Alzheimers Dis [Epub ahead of print]. [abstract] Mehrabadi M, Hussain M, Asgari S. (2013) MicroRNAome of Spodoptera frugiperda cells (Sf9) and its alteration following baculovirus infection. J Gen Virol [Epub ahead of print]. [abstract] microRNA Microarray Han H, Peng J, Hong Y,

Research teams led by scientists at the Zhejiang University, China have published several papers demonstrating the use of small RNA and degradome sequencing for discovery of novel miRNAs and their targets. In one study, researchers employed high-throughput sequencing and degradome analysis to identify miRNAs and their targets using high-throughput sequencing in cytoplasmic male sterility (CMS) and its maintainer fertile (MF) lines of Brassica juncea1. The researchers identified 197 known and 78 new candidate miRNAs during reproductive development of B. juncea. A total

Small RNA Sequencing Etebari K, Hussain M, Asgari S. (2013) Identification of microRNAs from Plutella xylostella larvae associated with parasitization by Diadegma semiclausum. Insect Biochem Mol Biol [Epub ahead of print]. [abstract]   Degradome Sequencing Yang J, Liu X, Xu B, Zhao N, Yang X, Zhang M. (2013) Identification of miRNAs and their targets using high-throughput sequencing and degradome analysis in cytoplasmic male-sterile and its maintainer fertile lines of brassica juncea. BMC

Previously, researchers at Wayne State University have showed that garcinol induces pancreatic cancer (PaCa) cell growth arrest and apoptosis in vitro. The present study, discusses chemo-sensitization by garcinol in synergism with first-line PaCa drug, gemcitabine. The miRNA expression profile of gemcitabine-resistant Panc-1 cells treated with garcinol and/or gemcitabine was also evaluated since alterations in microRNA (miRNA/miR) genes are of biological importance in the pathophysiology of cancers, including PaCa. Although growing evidence supports the role of miRNA in cancer, their response to

In this first study, genome-scale profiling of microRNA (miRNA) differential expression patterns in hESC neuronal lineage-specific progression was used by researchers at San Diego Regenerative Medicine Institute to identify molecular signatures of human embryonic neurogenesis1. These in vitro neuroectoderm-derived human neuronal cells have acquired a neuronal identity by down-regulating pluripotence-associated miRNAs and inducing the expression of miRNAs linked to regulating human CNS development to high levels in a stage-specific manner, including silencing of the prominent pluripotence-associated hsa-miR-302 family and drastic

MicroRNAs (miRNAs) play important roles in the regulation of rodent tooth development, but little is known about their role in tooth development in large mammals. Now, researchers at the Capital Medical University, Beijing, China have utilized a Seq-Array methodology and identified the specific microRNAome and expression profile in developing lower deciduous molars of the miniature pig, but they also provided useful information for investigating the molecular mechanism of tooth development in the miniature pig. The research team identified 637 unique